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Bromodeoxyuridine brdu kit

Manufactured by Roche
Sourced in Germany

The Bromodeoxyuridine (BrdU) kit is a laboratory product designed to detect and quantify cell proliferation. It provides a method for labeling and detecting cells undergoing DNA synthesis, which is a key indicator of cell division and proliferation.

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2 protocols using bromodeoxyuridine brdu kit

1

Molecular Regulation of Estrogen Receptor

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Culture media and growth supplements were obtained from Gibco (Germany). CdCl2 and melatonin were purchased from Sigma–Aldrich Company (Munich, Germany). Bromodeoxyuridine (BrdU) kit was purchased from Roche (Mannheim, Germany). Human antibody of glyceraldehyde-3 phosphate dehydrogenase (GAPDH) (G-9:sc-365062), mouse monoclonal IgG1 estrogen receptor α (sc-73479) and gout anti mouse IgG1-HRP (sc-2005) were purchased from Santa Cruz (USA).
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2

Molecular Mechanisms of Calpain-Mediated Injury

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ANG II, the calpain inhibitor MDL28170, the PI3K inhibitor LY294002, and fluorogenic peptide Suc-Leu-Leu-Val-Tyr-AMC were obtained from Calbiochem (La Jolla, CA). Anti-collagen-I antibody was obtained from Novus Biologicals (Littleton, CO). Antibodies against phospho-Akt (p-Akt) and total-Akt (t-Akt) were purchased from Cell Signaling Technology (Danvers, MA). NF-κB p65 was obtained from Santa Cruz Biotechnology (Dallas, TX). Antibodies against IκB-α and GAPDH were obtained from Epitomics (Burlingame, CA). Anti-calretinin antibody was purchased from BD Transduction (San Jose, CA). Bromodeoxyuridine (BrdU) kit was obtained from Roche (Mannheim, Germany). Enzyme-linked immunosorbent assay (ELISA) kits of ACE were from R&D Systems (Minneapolis, MN). The type 1 ANG II receptor antagonist losartan was obtained from Sigma-Aldrich (St. Louis, MO). Antibody specific for the calpain-mediated cleavage of spectrin (SBDP) was provided by Dr. Kevin K. W. Wang.
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