Saliva specimens were collected for NICU-HEALTH participants in the morning and afternoon on a single day each week during the NICU stay. Collections were conducted by trained study staff. The methods of sample collection, storage, and cortisol measurement have been described previously18 (link). In brief, saliva specimens were collected using a cotton swab (SalivaBio Infant Swab, Salimetrics) placed in the infant’s mouth for 2–5 min until the cotton swab was saturated. The swab was then centrifuged at 4,000 rotations per minute for 30 min, and extracted saliva was aliquotted to storage vials and frozen at −80 °C pending batch analysis. Saliva specimens were sent frozen to the Technical University of Dresden for cortisol measurement using a commercially available chemiluminescence assay38 –39 (link).
Salivabio infant swab
Manufactured by Salimetrics
Sourced in United States
The SalivaBio Infant Swab is a laboratory tool designed to collect saliva samples from infants. It consists of a soft, absorbent swab attached to a handle, intended to gently and safely obtain saliva specimens for analysis.
Automatically generated - may contain errors
Lab products found in correlation
7 protocols using salivabio infant swab
1
Saliva Cortisol Measurement in NICU Infants
2
Saliva Cortisol Measurement in NICU Infants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Saliva specimens were collected for NICU-HEALTH participants in the morning and afternoon on a single day each week during the NICU stay. Collections were conducted by trained study staff. The methods of sample collection, storage, and cortisol measurement have been described previously18 (link). In brief, saliva specimens were collected using a cotton swab (SalivaBio Infant Swab, Salimetrics) placed in the infant’s mouth for 2–5 min until the cotton swab was saturated. The swab was then centrifuged at 4,000 rotations per minute for 30 min, and extracted saliva was aliquotted to storage vials and frozen at −80 °C pending batch analysis. Saliva specimens were sent frozen to the Technical University of Dresden for cortisol measurement using a commercially available chemiluminescence assay38 –39 (link).
3
Saliva Collection for Infant Health Assessment
4
Saliva Collection for Infant Health Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Saliva collection was performed for all participants in NICU-HEALTH at two time points on a single day each week during the NICU hospitalization by study staff. The time of saliva collection was recorded and used as the “start” of the 6-hour look-back for NISS score calculation as described above. A cotton swab (SalivaBio Infant Swab, Salimetrics) was placed in the infant’s mouth for 2-5 minutes until saturated with saliva. The swab was then centrifuged at 4000 rotations per minute for 30 minutes and extracted saliva was aliquoted to storage vials and frozen at −80°C pending batch analysis.
5
Salivary and Plasma Clonazepam Pharmacokinetics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
This was an open‐label, single‐dose study in 20 healthy subjects aged 18‐30. Exclusion criteria were history of disease or use of medications that might interfere with saliva production, such as opiates and anticholinergics. Subjects were asked to refrain from alcohol and caffeine use for 24 hours prior to drug administration until the end of the study, and from any nutrients with Cytochrome P450 (CYP)‐modulating activity for 3 days prior to drug administration. Subjects were administered a single dose of 0.5 mg (n = 10) or 1.0 mg (n = 10) clonazepam solution (Rivotril) dissolved in lemonade, after which paired plasma and saliva samples were taken at 0.5, 1, 2, 4, 6, 8, 24 and 48 hours post dose. Subjects thoroughly rinsed their mouths with water 10 minutes prior to saliva sampling. Saliva samples were obtained using the SalivaBio Infant Swab (Salimetrics, Carlsbad, CA, USA) according to the manufacturer's instructions.
6
Canine Saliva Collection: Passive and Active Phases
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Passive phase: E came into the testing area and collected saliva samples from the dog immediately after the owner or the chief manager left the room (phase 1, P1). Saliva was collected by keeping a SalivaBio
infant swab (Salimetrics, Carlsbad, CA, U.S.A.) in the dog’s mouth for 1 min. Restraint during sampling without making friendly visual contact, physical contact or name calling was kept to a minimum. After the first sampling, E
sat in the chair for 2 min without moving and made visual contact with the dog. When the dog initiated physical contact with E in any way, E petted the dog’s head or chin once.
Active phase: After the 2-min passive phase, E stood while making eye contact with the dog and calling him or her by name in a friendly manner. If the dog initiated physical contact with E in any way, E petted
the dog’s head or chin once. If the dog did not come close to E, the E repeated the dog’s name 3 times at intervals of 10 sec. For the S group, the names of dogs that were called out during the experiment were established during
their first visit to the center so that they would be familiar to the dogs. After the 2-min active phase (phase 2, P2), saliva was collected in the same manner described previously.
infant swab (Salimetrics, Carlsbad, CA, U.S.A.) in the dog’s mouth for 1 min. Restraint during sampling without making friendly visual contact, physical contact or name calling was kept to a minimum. After the first sampling, E
sat in the chair for 2 min without moving and made visual contact with the dog. When the dog initiated physical contact with E in any way, E petted the dog’s head or chin once.
Active phase: After the 2-min passive phase, E stood while making eye contact with the dog and calling him or her by name in a friendly manner. If the dog initiated physical contact with E in any way, E petted
the dog’s head or chin once. If the dog did not come close to E, the E repeated the dog’s name 3 times at intervals of 10 sec. For the S group, the names of dogs that were called out during the experiment were established during
their first visit to the center so that they would be familiar to the dogs. After the 2-min active phase (phase 2, P2), saliva was collected in the same manner described previously.
7
Infant Saliva Cortisol Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A liquid saliva sample will be collected 8 weeks after birth using sterile SalivaBio’s Children’s Swab (Salimetrics, USA) and SalivaBio Infant Swab (Salimetrics) from the mother and the infant, respectively. The liquid samples retrieved from the swab by centrifugation (2683 g for 10 min) will be stored at the laboratory at −80°C until the measurement of salivary cortisol levels using the commercial kit (Cortisol ELISA kit, Enzo).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!