Human il 4 quantikine hs elisa kit

The measurement of serum cytokines concentrations was determined with the usage of a quantitative commercial enzyme-linked immunosorbent assay (ELISA) technique, following the manufacturers’ recommendations. In brief, the serum concentration of IL-2 was measured using a Human IL-2 Quantikine ELISA Kit (sensitivity, 7 pg/mL; R&D Systems, Minneapolis, MN, USA); the serum concentration of IL-4 was determined using the Human IL-4 Quantikine HS ELISA Kit (sensitivity, 0.22 pg/mL; R&D Systems, USA); the concentration of serum IL-6 was measured using the Human IL-6 Quantikine HS ELISA Kit (sensitivity, 0.11 pg/mL; R&D Systems, USA); the serum concentration of IL-10 was determined using the Human IL-10 Quantikine HS ELISA Kit (sensitivity, 0.17 pg/mL; R&D Systems, USA), and the concentration of serum IFN-gamma was determined using the Human IFN-γ Quantikine ELISA Kit (sensitivity, 8 pg/mL; R&D Systems, USA). The results were measured with an automatic reader VICTOR3 (Perkin Elmer, Waltham, MA, USA); that action is based on the measurements of the light absorbance of the tested material and its comparison with control samples of known concentration. The WorkOut computer program, working with the reader on the basis of known concentrations, plotted linear curves on the basis of which the concentration of cytokines in the tested samples was calculated.