Alexa fluor 594 donkey anti mouse igg h l

Manufactured by Jackson ImmunoResearch

Alexa Fluor 594 donkey anti–mouse IgG (H+L) is a secondary antibody conjugate. It is designed to detect and visualize mouse immunoglobulin G (IgG) in various applications such as immunohistochemistry, flow cytometry, and Western blotting.

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Lab products found in correlation

Ab138513, by Abcam (1 mentions) Cy3 conjugated affinipure donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions) 510 meta, by Zeiss (1 mentions) β catenin, by Thermo Fisher Scientific (1 mentions) Anti mouse igg hrp, by GE Healthcare (1 mentions) Alexa fluor 488 goat anti mouse igg h l, by Thermo Fisher Scientific (1 mentions) Superscript 3 reverse transcriptase, by Thermo Fisher Scientific (1 mentions) Quikchange 2 xl site directed mutagenesis kit, by Agilent Technologies (1 mentions) Goat anti rabbit igg h l alexa fluor 488, by Abcam (1 mentions) Operetta cls system, by PerkinElmer (1 mentions)

Close spelling variants of this product

Alexa Fluor® 594 AffiniPure Donkey Anti-Mouse IgG (H+L) (3 citations) Anti-mouse IgG (H + L) Alexa Fluor® 594 (1 citations) Alexa Fluor®594 Donkey Anti-Mouse IgG (6 citations) Alexa-Fluor 594 donkey anti-mouse (15 citations) Alexa Fluor® 594 anti-mouse IgG (4 citations) Donkey anti-Mouse IgG (H+L); (7 citations) Alexa Fluor donkey anti-mouse IgG (3 citations) Alexa-594 donkey anti-mouse (4 citations) Anti-mouse Alexa Fluor 594 (10 citations) Alexa Fluor 594 (140 citations)

3 protocols using alexa fluor 594 donkey anti mouse igg h l

Histological and Immunofluorescence Analysis of Skin Samples

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Skin samples were fixed in 4% paraformaldehyde overnight at 4 °C, serially dehydrated and embedded in
paraffin. The specimens were sectioned and stained with hematoxylin and eosin (H&E). Immunofluorescence staining was performed
with routine antigen retrieval as suggested by the antibody manufacturer [41 (link)]. The slides were examined under a confocal microscope (Meta 510, Carl Zeiss). The primary antibodies for
immunostaining used in this study were as follows: fibrinogen beta-chain fragment (Abcam, EPR3083; 1:200), sulfated glycoprotein 1
(Abcam, ab68466; 1:200), galectin-1 (Abcam, ab138513; 1:200), lumican (Abcam, ab168348; 1:200), annexin A2 (Genetex, GTX101902,
1:200), gelsolin (Genetex, GTX101185; 1:200), apolipoprotein-A1 (Novus, NBP1-77008; 1:200) and fibronectin (Genetex, GTX61207;
1:200). Secondary antibodies were Alexa Fluor 594 donkey anti–mouse IgG (H+L) (Jackson ImmunoResearch,
715-585-151; 1:500), Cy3-conjugated AffiniPure donkey anti–rabbit IgG (Jackson ImmunoResearch, 711-165-152; 1:500) and
Alexa Fluor 594-AffiniPure F (ab′)2 Fragment rabbit anti–chicken IgY (IgG) (H+L) (Jackson ImmunoResearch,
303-586-003; 1:500). β-galactosidase activity and alkaline phosphatase activity were detected as described [43 (link),44 (link)].

Fan S.M., Tsai C.F., Yen C.M., Lin M.H., Wang W.H., Chan C.C., Chen C.L., Phua K.K., Pan S.H., Plikus M.V., Yu S.L., Chen Y.J, & Lin S.J. (2018). Inducing hair follicle neogenesis with secreted proteins enriched in embryonic skin. Biomaterials, 167, 121-131.

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Modulating SOX17 and β-Catenin Signaling

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The following antibodies were used: c-Myc (9E10) (sc-40, Santa Cruz Biotechnology, Santa Cruz, CA); SOX17 (AF1974, R&D systems, Minneapolis, MN); pan-cytokeratin (sc-81714, Santa Cruz Biotechnology, Santa Cruz, CA); β-catenin (zymed 13-8400, Thermo Fisher, Waltham, MA) vinculin (v4139, Sigma-Aldrich, St. Louis, MO); HRP-anti-mouse IgG (NA934V, GE Healthcare); Alexa Fluor-488 goat anti-mouse IgG (H+L) (A11029, Invitrogen, Carlsbad, CA), Alexa Fluor-488 donkey anti-goat IgG (H+L) (705-546-147, Jackson ImmunoResearch Laboratories, West Grove, PA); Alexa Fluor-594 donkey anti-mouse IgG (H+L) (715-586-150, Jackson ImmunoResearch Laboratories, West Grove, PA).
The coding sequencing of WT SOX17 was PCR-amplified from human tumor cDNA synthesized using superscript III reverse transcriptase (Thermo Fisher, Waltham, MA), and cloned into the pCDH-CMV-MCS-EF1-GreenPuro plasmid (SystemsBiosciences Palo Alto, CA). Mutations were introduced using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). A mutant β-catenin (S33A, S37A, T41A, S45A) expression construct, SOX17 compressed motif reporter [16 (link)] and pBAR TCF/β-catenin activated reporter [35 (link)] were obtained from addgene.

Walker C.J., O'Hern M.J., Serna V.A., Kurita T., Miranda M.A., Sapp C.E., Mutch D.G., Cohn D.E, & Goodfellow P.J. (2017). Novel SOX17 frameshift mutations in endometrial cancer are functionally distinct from recurrent missense mutations. Oncotarget, 8(40), 68758-68768.

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TGF-β1 and Omentin-1 Effects on Cell Markers

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Eight hundred starved cells were seeded into each well of a 96-wells plate, and treated for 24 h with recombinant human TGF-β1 and recombinant human omentin-1 at the concentration mentioned above. After incubation, cells were rinsed 3 times with phosphate-buffered saline (PBS), fixed with 4% paraformaldehyde for 20 min, permeated with 0.5% Triton-X100 for 15 min, blocked with normal goat serum for 60 min, and incubated overnight at 4 °C with primary antibodies against the following proteins: α-SMA (1:200, dilution), vimentin (1:200, dilution), and VE-Cad (1:100, dilution). This was followed by incubation for 40 min at 37 °C with corresponding secondary antibodies: Alexa Fluor 488 goat-anti-rabbit IgG H&L (1:500 dilution, ab150077, Abcam) and Alexa Fluor 594 donkey-anti-mouse IgG (H + L) (1:500 dilution, 715–585-150, Jackson Laboratories). Thereafter, cells were stained for 5 min with 4′,6-diamidino-2-phenylindole (DAPI, 1:1000 dilution, 564,907, BD Pharmingen), and images were then acquired using the PerkinElmer Operetta CLS system. The fluorescence intensity was analyzed using Harmony 3.5 software.

Chen Y., Liu F., Han F., Lv L., Tang C.E., Xie Z, & Luo F. (2020). Omentin-1 is associated with atrial fibrillation in patients with cardiac valve disease. BMC Cardiovascular Disorders, 20, 214.

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