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Beta actin n 21

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Beta-Actin (N-21) is a primary antibody produced by Santa Cruz Biotechnology. It is designed to detect beta-actin, a highly conserved cytoskeletal protein found in eukaryotic cells. The antibody recognizes the N-terminal region of the beta-actin protein.

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Lab products found in correlation

Alpha tubulin ab80779, by Abcam (2 mentions) Psmc2 mss1 104, by Enzo Life Sciences (2 mentions) Psmd1 c 7, by Santa Cruz Biotechnology (2 mentions) Np0009, by Thermo Fisher Scientific (2 mentions) Erα f 10, by Santa Cruz Biotechnology (2 mentions) Odyssey clx imaging machine, by LI COR (2 mentions) Image studio software, by LI COR (2 mentions) Image lab software, by Bio-Rad (2 mentions) Protease inhibitor cocktail, by Roche (2 mentions) Chemidoc mp system, by Bio-Rad (2 mentions) C abl, by Cell Signaling Technology (1 mentions) Phosphotyrosine 4g10, by Merck Group (1 mentions) Alpha tubulin b 7, by Santa Cruz Biotechnology (1 mentions) Hsp90, by Cell Signaling Technology (1 mentions) Stat3, by Cell Signaling Technology (1 mentions) Imatinib, by LC Laboratories (1 mentions)

3 protocols using beta actin n 21

1

Quantitative Immunoblotting of PSMC2, PSMD2, and ERα

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For PSMC2 and PSMD2 immunoblotting, cells were lysed in HENG buffer (50mM Hepes-KOH pH 7.9, 150mM NaCl, 2mM EDTA pH 8.0, 20mM sodium molybdate, 0.5% Triton X-100, 5% glycerol), with protease inhibitor cocktail (Roche Diagnostics #11836153001). Protein concentration was determined by the BCA assay (Thermo-Fisher Scientific #23227), and proteins were resolved on SDS-PAGE for immunoblot analysis. Antibodies against the following human proteins were used: alpha-Tubulin (ab80779; Abcam), PSMC2 (MSS1–104; Enzo Life Sciences) and PSMD1 (C-7; Santa-Cruz). Visualization was performed using the ChemiDoc MP System (Bio-Rad), and ImageLab Software (Bio-Rad) was used to quantify relative band intensities. For ERα immunonblotting, cells were lysed with a mix of 4X protein loading buffer (Li-Cor 928–40004) and 10X NuPAGE sample reducing agent (Life Technologies NP0009). Protein concentration was normalized by cell counting, and proteins were resolved on SDS-PAGE. Antibodies against the following human proteins were used: beta-Actin (N-21; Santa Cruz), ERα (F-10; Santa Cruz). Visualization was performed using the Odyssey CLx imaging machine (Li-Cor), and Image Studio Software (Li-Cor) was used to quantify the relative intensities.
Ben-David U., Siranosian B., Ha G., Tang H., Oren Y., Hinohara K., Strathdee C.A., Dempster J., Lyons N.J., Burns R., Nag A., Kugener G., Cimini B., Tsvetkov P., Maruvka Y.E., O’Rourke R., Garrity A., Tubelli A.A., Bandopadhayay P., Tsherniak A., Vazquez F., Wong B., Birger C., Ghandi M., Thorner A.R., Bittker J.A., Meyerson M., Getz G., Beroukhim R, & Golub T.R. (2018). Genetic and transcriptional evolution alters cancer cell line drug response. Nature, 560(7718), 325-330.
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2

Quantitative Immunoblotting of PSMC2, PSMD2, and ERα

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For PSMC2 and PSMD2 immunoblotting, cells were lysed in HENG buffer (50mM Hepes-KOH pH 7.9, 150mM NaCl, 2mM EDTA pH 8.0, 20mM sodium molybdate, 0.5% Triton X-100, 5% glycerol), with protease inhibitor cocktail (Roche Diagnostics #11836153001). Protein concentration was determined by the BCA assay (Thermo-Fisher Scientific #23227), and proteins were resolved on SDS-PAGE for immunoblot analysis. Antibodies against the following human proteins were used: alpha-Tubulin (ab80779; Abcam), PSMC2 (MSS1–104; Enzo Life Sciences) and PSMD1 (C-7; Santa-Cruz). Visualization was performed using the ChemiDoc MP System (Bio-Rad), and ImageLab Software (Bio-Rad) was used to quantify relative band intensities. For ERα immunonblotting, cells were lysed with a mix of 4X protein loading buffer (Li-Cor 928–40004) and 10X NuPAGE sample reducing agent (Life Technologies NP0009). Protein concentration was normalized by cell counting, and proteins were resolved on SDS-PAGE. Antibodies against the following human proteins were used: beta-Actin (N-21; Santa Cruz), ERα (F-10; Santa Cruz). Visualization was performed using the Odyssey CLx imaging machine (Li-Cor), and Image Studio Software (Li-Cor) was used to quantify the relative intensities.
Ben-David U., Siranosian B., Ha G., Tang H., Oren Y., Hinohara K., Strathdee C.A., Dempster J., Lyons N.J., Burns R., Nag A., Kugener G., Cimini B., Tsvetkov P., Maruvka Y.E., O’Rourke R., Garrity A., Tubelli A.A., Bandopadhayay P., Tsherniak A., Vazquez F., Wong B., Birger C., Ghandi M., Thorner A.R., Bittker J.A., Meyerson M., Getz G., Beroukhim R, & Golub T.R. (2018). Genetic and transcriptional evolution alters cancer cell line drug response. Nature, 560(7718), 325-330.
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3

Investigating AUY922 and Imatinib Effects

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The-AUY922 compound was kindly provided by Novartis institution for BioMedical Research. Imatinib was purchased from LC laboratories. Imatinib was diluted in PBS and AUY922 was dissolved in 100% DMSO (dimethyl sulfoxide) at the stocking concentration of 10 mM, and was aliquoted into microtubes to be stored at −20°C until use. Commercially available antibodies used were anti-phospho-Jak2Y1007 (Mollipore, Cat# 04-1098), Stat3 (Cell signaling, Cat# 9132), Akt (Cell signaling, Cat# 9272), HSP90 (Cell signaling, Cat# 4874), c-Abl (Cell Signaling, Cat# 2862), phosphotyrosine (4G10) (Millipore, Cat# 05-321), alpha tubulin (B-7) (Santa Cruz Biotechnology, Cat# sc-5286), beta actin (N-21) (Santa Cruz Biotechnology, Cat# sc-130656). Sepharose beads conjugated Jak2 antibody was purchase from Cell Signaling (Cat# 4089). Anti-Abl SH2 domain monoclonal antibody (8E9) was produced by our own lab.
Tao W., Chakraborty S.N., Leng X., Ma H, & Arlinghaus R.B. (2015). HSP90 inhibitor AUY922 induces cell death by disruption of the Bcr-Abl, Jak2 and HSP90 signaling network complex in leukemia cells. Genes & Cancer, 6(1-2), 19-29.
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