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Gst sc 459

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GST (sc-459) is a recombinant protein that is commonly used in biochemical research. It serves as an affinity tag, facilitating the purification of target proteins fused to the GST (Glutathione S-Transferase) domain. The GST domain binds to immobilized glutathione, allowing for the separation and enrichment of the fusion protein from complex mixtures.

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Lab products found in correlation

Polyclonal raco 1 hpa030098 and trim7 hpa039213 antibodies, by Merck Group (2 mentions) Jun 610326, by BD (2 mentions) Flag a8592 and ha h 6908, by Merck Group (2 mentions)

Spelling variants of this product

Sc-459 (12 citations) Anti-GST (sc-459) (6 citations) Rabbit anti-GST (sc-459, (3 citations) Anti-GST (sc-459) antibody (2 citations)

2 protocols using gst sc 459

1

Characterization of RACO-1 and TRIM7 in Lung Cancer

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Human embryonic kidney (HEK) 293T cells, human lung adenocarcinoma H727 cells and primary murine lung adenocarcinoma cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal calf serum (FCS). HEK293T and H727 cells were obtained from Cancer Research UK Cell Services. Polyclonal RACO-1 (HPA030098) and Trim7 (HPA039213) antibodies were from Sigma. Antibodies against p-ERK1/2 (9106), total ERK1/2 (9102), pCREB (9191), total CREB (9197), MSK1 (3489) and MSK2 (3679) were from Cell Signaling Technology; Jun (610326) from BD transduction laboratories; Flag (A8592) and HA (H 6908) from Sigma; β-actin (ab49900), GFP (ab290) from Abcam; and GST (sc-459) from Santa Cruz. Mouse monoclonal antibody against Myc tag was generated in-house. Anti-FLAG HRP antibody was used at 1:5000 dilution and anti- β-actin HRP antibody was used at 1:30,000 dilution. All other antibodies were used at 1:1000 dilution for western blots. Uncropped scans of western blots are shown in Supplementary Fig. 6.
Chakraborty A., Diefenbacher M.E., Mylona A., Kassel O, & Behrens A. (2015). The E3 ubiquitin ligase Trim7 mediates c-Jun/AP-1 activation by Ras signalling. Nature communications, 6, 6782.
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2

Characterization of RACO-1 and TRIM7 in Lung Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human embryonic kidney (HEK) 293T cells, human lung adenocarcinoma H727 cells and primary murine lung adenocarcinoma cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal calf serum (FCS). HEK293T and H727 cells were obtained from Cancer Research UK Cell Services. Polyclonal RACO-1 (HPA030098) and Trim7 (HPA039213) antibodies were from Sigma. Antibodies against p-ERK1/2 (9106), total ERK1/2 (9102), pCREB (9191), total CREB (9197), MSK1 (3489) and MSK2 (3679) were from Cell Signaling Technology; Jun (610326) from BD transduction laboratories; Flag (A8592) and HA (H 6908) from Sigma; β-actin (ab49900), GFP (ab290) from Abcam; and GST (sc-459) from Santa Cruz. Mouse monoclonal antibody against Myc tag was generated in-house. Anti-FLAG HRP antibody was used at 1:5000 dilution and anti- β-actin HRP antibody was used at 1:30,000 dilution. All other antibodies were used at 1:1000 dilution for western blots. Uncropped scans of western blots are shown in Supplementary Fig. 6.
Chakraborty A., Diefenbacher M.E., Mylona A., Kassel O, & Behrens A. (2015). The E3 ubiquitin ligase Trim7 mediates c-Jun/AP-1 activation by Ras signalling. Nature communications, 6, 6782.
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