Log In Sign up
The largest database of trusted experimental protocols

S 2 2 5 5 tetramethyl 2 5 dihydro 1h pyrrol 3 yl methyl methanesulfonothioate mtssl spin label

Manufactured by LGC
Visit Supplier
Sourced in Canada

S-(2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl methanesulfonothioate (MTSSL) is a spin label used in various scientific applications. It functions as a stable free radical that can be attached to specific sites within a molecule or protein, allowing for the measurement of structural and dynamic properties using electron paramagnetic resonance (EPR) spectroscopy.

Automatically generated - may contain errors

Lab products found in correlation

Isopropyl β d thiogalactoside, by Formedium (3 mentions) N dodecyl β d maltopyranoside ddm, by Anatrace (2 mentions) N decyl β d maltopyranoside anagrade, by Anatrace (2 mentions) E coli polar extract, by Avanti Polar Lipids (2 mentions) Lpc 18 1, by Avanti Polar Lipids (2 mentions) Lpc 14 0, by Avanti Polar Lipids (2 mentions) Tris 2 carboxyethyl phosphine tcep, by Thermo Fisher Scientific (1 mentions) Bio beads, by Bio-Rad (1 mentions)

3 protocols using s 2 2 5 5 tetramethyl 2 5 dihydro 1h pyrrol 3 yl methyl methanesulfonothioate mtssl spin label

1

Membrane Protein Reconstitution Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
n-Dodecyl-β-D-maltopyranoside (DDM) anagrade was obtained from Anatrace (Maumee, OH) or Glycon (Luckenwalde, Germany). Isopropyl-β-D-thiogalactoside was obtained from Formedium (Norfolk, UK), and tris(2-carboxyethyl)phosphine (TCEP) was obtained from Thermo Fisher Scientific (Waltham, MA). The S-(2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl methanesulfonothioate (MTSSL) spin label was obtained from Toronto Research Chemicals (North York, Canada), dimyristoyl phosphatidylcholine (DMPC) (14:0/14:0) was purchased from Avanti Polar Lipids (Alabaster, AL), and Biobeads were purchased from Bio-Rad Laboratories (Watford, UK). All other chemicals were obtained from Sigma-Aldrich (Dorset, UK) unless otherwise stated.
Kapsalis C., Ma Y., Bode B.E, & Pliotas C. (2020). In-Lipid Structure of Pressure-Sensitive Domains Hints Mechanosensitive Channel Functional Diversity. Biophysical Journal, 119(2), 448-459.
+ Open protocol
+ Expand
2

Membrane Protein Preparation and Labeling

Check if the same lab product or an alternative is used in the 5 most similar protocols
n-Dodecyl-β-D-maltopyranoside (DDM) and n-decyl-β-D-maltopyranoside (DM) anagrade were obtained from Anatrace Inc or Glycon (Germany). Isopropyl-β-D-thiogalactoside (IPTG) was obtained from Formedium and (tris(2-carboxyethyl)phosphine (TCEP) from Thermo Scientific Ltd. The S-(2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl methanesulfonothioate (MTSSL) spin label was obtained from Toronto Research chemicals, Toronto. Phospholipids, E. coli Polar Extract, PC, PE, PG, LPC 18:1 and LPC (14:0) were purchased from Avanti Polar Lipids. All other chemicals unless otherwise stated were obtained from Sigma. Mutants were generated with the Stratagene QuickChange™ protocol as described previously12 ,19 (link).
Pliotas C., Dahl A.C., Rasmussen T., Mahendran K.R., Smith T.K., Marius P., Gault J., Banda T., Rasmussen A., Miller S., Robinson C.V., Bayley H., Sansom M.S., Booth I.R, & Naismith J.H. (2015). The role of lipids in mechanosensation. Nature structural & molecular biology, 22(12), 991-998.
+ Open protocol
+ Expand
3

Membrane Protein Preparation and Labeling

Check if the same lab product or an alternative is used in the 5 most similar protocols
n-Dodecyl-β-D-maltopyranoside (DDM) and n-decyl-β-D-maltopyranoside (DM) anagrade were obtained from Anatrace Inc or Glycon (Germany). Isopropyl-β-D-thiogalactoside (IPTG) was obtained from Formedium and (tris(2-carboxyethyl)phosphine (TCEP) from Thermo Scientific Ltd. The S-(2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl methanesulfonothioate (MTSSL) spin label was obtained from Toronto Research chemicals, Toronto. Phospholipids, E. coli Polar Extract, PC, PE, PG, LPC 18:1 and LPC (14:0) were purchased from Avanti Polar Lipids. All other chemicals unless otherwise stated were obtained from Sigma. Mutants were generated with the Stratagene QuickChange™ protocol as described previously12 ,19 (link).
Pliotas C., Dahl A.C., Rasmussen T., Mahendran K.R., Smith T.K., Marius P., Gault J., Banda T., Rasmussen A., Miller S., Robinson C.V., Bayley H., Sansom M.S., Booth I.R, & Naismith J.H. (2015). The role of lipids in mechanosensation. Nature structural & molecular biology, 22(12), 991-998.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!