Rat anti ha mab clone 3f10

Manufactured by Roche

Sourced in United States

Rat anti-HA mAb clone 3F10 is a monoclonal antibody used for the detection and purification of proteins tagged with the HA (Hemagglutinin) epitope. It binds specifically to the HA tag, which is a commonly used protein tag for recombinant protein expression.

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Lab products found in correlation

Anti ha, by Thermo Fisher Scientific (2 mentions) Mouse anti flag mab clone m2, by Merck Group (2 mentions) Mouse anti resa mab clone 28 234, by Merck Group (2 mentions) Anti ha, by Merck Group (2 mentions) Anti e cadherin mab, by BD (1 mentions) Anti β actin mab clone ac 15, by Merck Group (1 mentions) Anti occludin 711500, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Rat anti-HA MAb 3F10 Rat anti-HA clone 3F10 HA (3F10) rat mAb Rat anti-HA 3F10 Anti-HA (clone 3F10; HA mAB 3F10 Rat mAb (3F10; HA (clone 3F10; Rat anti-HA mAb Anti-HA (3F10,

4 protocols using rat anti ha mab clone 3f10

Comprehensive Antibody Characterization for Malaria Research

Cited 2 times

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The following antibodies were used for IFA and Western blot: rabbit polyclonal anti-HA (Life Technologies) (IFA: 1:100); rabbit polyclonal anti-HA (Sigma) (WB 1:1000); rat anti-HA mAb clone 3F10 (Roche) (IFA: 1:100, WB: 1:1000); mouse anti-FLAG mAb clone M2 (Sigma) (IFA: 1:100, WB 1:500); mouse anti-EXP2 mAb clone 7.7^{33 (link)} (IFA: 1:1000, WB: 1:1000); mouse anti-HRP2 mAb 2G12^{14 (link)} (IFA 1:1000, WB: 1:500); mouse anti-RESA mAb clone 28/2³⁴ (IFA: 1:1000, WB: 1:1000); rabbit polyclonal anti-BiP^{35 (link)} (WB: 1:1000); rabbit polyclonal anti-REX1^{36 (link)} (IFA: 1:1000); mouse polyclonal anti-REX2^{15 (link)} (IFA: 1:500); mouse polyclonal anti-REX3^{15 (link)} (IFA: 1:500); rabbit polyclonal anti-SBP1^{21 (link)} (IFA: 1:500); mouse polyclonal anti-MSRP6^{22 (link)} (IFA: 1:500); mouse polyclonal anti-PfGECO^{27 (link)} (IFA: 1:300); rabbit polyclonal anti-Pfs16^{37 (link)} (IFA: 1:500); rabbit polyclonal anti-PfEMP1 ATS^{38 (link)} (IFA: 1:200); mouse polyclonal anti-CLAG3^{28 (link)} (IFA: 1:300); rabbit polyclonal anti-PM2 antibody 737^{39 (link)} (WB 1:2000); rabbit polyclonal anti-KAHRP^{16 (link)} (IFA: 1:500); rabbit polyclonal anti-SERP^{40 (link)} (IFA: 1:500, WB: 1:1000).

Beck J.R., Muralidharan V., Oksman A, & Goldberg D.E. (2014). HSP101/PTEX mediates export of diverse malaria effector proteins into the host erythrocyte. Nature, 511(7511), 592-595.

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Antibody Characterization for Epithelial Markers

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Affinity-purified polyclonal rabbit anti-EpCAM antibody (Ab) has been described previously [22 (link)]. Monoclonal anti-mouse TROP2 antibody was generated by immunizing rabbits with recombinant protein comprised of the extracellular region of mouse TROP2 fused by human IgG Fc. Polyclonal anti-TROP2, polyclonal goat anti-human HAI-1 (AF1048), sheep anti-matriptase (AF3946), goat anti-mouse HAI-1 (AF1141), and goat anti-mouse HAI-2 (AF1107) Abs were purchased from R & D Systems (Minneapolis, MN, USA). Anti-EpCAM (PA5-19832), anti-claudin-1 (717800), anti-claudin-7 (349100), and anti-occludin (711500) Abs were from Thermo Fisher Scientific (Carlsbad, CA, USA). Rabbit anti-matriptase Ab (IM1014) was from EMD Millipore (Temecula, CA, USA). Polyclonal anti-HAI-2 Ab (HPA011101), mouse anti-Flag mAb (clone M2) and anti-β-actin mAb (clone AC-15) were from Sigma (St. Louis, MO, USA). Anti-E-cadherin mAb was from BD Biosciences (San Jose, CA, USA), and rat anti-HA mAb (clone 3F10) was from Roche (Indianapolis, IN, USA).

Wu C.J., Lu M., Feng X., Nakato G, & Udey M.C. (2020). Matriptase Cleaves EpCAM and TROP2 in Keratinocytes, Destabilizing Both Proteins and Associated Claudins. Cells, 9(4), 1027.

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Comprehensive Antibody Characterization for Malaria Research

Cited 2 times

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Beck J.R., Muralidharan V., Oksman A, & Goldberg D.E. (2014). HSP101/PTEX mediates export of diverse malaria effector proteins into the host erythrocyte. Nature, 511(7511), 592-595.

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Subcellular Fractionation of GlDRP Proteins

Cited 1 time

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For sub-cellular fraction experiments, 4^.10⁶GlDRP-HA and GlDRP-K43E-HA- expressing transgenic cells were lysed by freeze-thawing and supernatant (soluble fraction) and pellet (membrane fraction) were prepared by centrifugation at 14’000 x g for 10 minutes at 4°C. The HA-tagged proteins were detected by SDS-PAGE and Western blot using a rat anti-HA mAb (clone 3F10, Roche) as described previously [54 (link)].

Rout S., Zumthor J.P., Schraner E.M., Faso C, & Hehl A.B. (2016). An Interactome-Centered Protein Discovery Approach Reveals Novel Components Involved in Mitosome Function and Homeostasis in Giardia lamblia. PLoS Pathogens, 12(12), e1006036.

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