Chromatin immunoprecipitations (ChIPs) and the quantification of immunoprecipitated DNA sequences by Q-PCR were performed as described previously (36 (link)). The localization of E2F motifs in E2F7-regulated miRNAs was carried out with the MotifLocator tool of the TOUCAN program (37 (link)). The search was restricted to the proximal promoter region (−1000 and +500 bp relative to the transcription start site) (38 (link)). Sequences of Q-PCR primers are listed in Supplementary Table S3. Antibodies used for ChIP analysis were: E2F1 (sc-193, Santa Cruz), E2F2 (sc-633, Santa Cruz), E2F3 (sc-878, Santa Cruz), E2F4 (sc-1082, Santa Cruz), E2F7 (sc-66870, Santa Cruz), RB (sc-50 Santa Cruz), p107 (sc-318 Santa Cruz), p130 (sc-317 Santa Cruz), MYC (sc-764 Santa Cruz), RNA polymerase II (sc-899, Santa Cruz) and SV40LT (sc-147, Santa Cruz).
E2f2 sc 633
Manufactured by Santa Cruz Biotechnology
Sourced in United States
E2F2 (sc-633) is a laboratory reagent produced by Santa Cruz Biotechnology. It is an antibody that recognizes the E2F2 protein, which is a transcription factor involved in the regulation of cell cycle progression and cellular proliferation. The product is intended for research use only and its core function is to detect and study the E2F2 protein in various experimental systems.
Automatically generated - may contain errors
2 protocols using e2f2 sc 633
1
Quantifying E2F-Regulated miRNAs by ChIP-qPCR
2
Chromatin Immunoprecipitation and qPCR Analysis
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Chromatin immunoprecipitations and the quantification of immunoprecipitate-enriched DNA sequences by real-time PCR were performed as described previously [43 (link)]. Sequences of PCR primers are listed in Supplementary Table S4 . Antibodies used were E2F1 (sc-251), E2F2 (sc-633), and SV40Tag (sc-147), all from Santa Cruz Biotechnologies (Santa Cruz, CA, USA).
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