Triethylamine tea

Manufactured by Avantor

Sourced in United States, Germany

Triethylamine (TEA) is a clear, colorless, flammable liquid commonly used as a solvent, catalyst, and reagent in various chemical processes. It has a distinctive amine-like odor. TEA is a versatile compound with applications in organic synthesis, pharmaceutical manufacturing, and other industrial sectors. As a chemical reagent, it plays a role in the synthesis of pharmaceuticals, agrochemicals, and other specialty chemicals.

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6 protocols using triethylamine tea

Synthesis of CNC Suspension

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CNC suspension (solid content of 10.4% (w/w)) was purchased from the University of Maine (Orono, ME, USA), which was prepared using the sulfuric acid method. Acetone (≥99%), toluene (≥99%), triethylamine (TEA) (≥99%), DMSO (≥99%), chloroform (≥99%), and hydrochloric acid (37%) were purchased from VWR (Darmstadt, Germany). 2,4-toluene diisocyanate (≥98%) was purchased from TCI Chemicals (Eschborn, Germany) and was stored in sealed bottles in the fridge. toluene, TEA, and DMSO were stored over A4 molecular sieve, while Acetone was stored under A3 molecular sieve. Both molecular sieves were purchased from Carl Roth (Karlsruhe, Germany) and regenerated before use.

, & Abushammala H. (2019). On the Para/Ortho Reactivity of Isocyanate Groups during the Carbamation of Cellulose Nanocrystals Using 2,4-Toluene Diisocyanate. Polymers, 11(7), 1164.

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Synthesis of CNC-Based Polymer Composites

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The CNC suspension used in this paper had a solid content of 10.4% (w/w) and was purchased from the University of Maine (Maine, USA), which was prepared using the sulfuric acid method. Acetone (>99%), chloroform (>99%), toluene (>99%), dimethylsulfoxide (DMSO) (>99.9%), triethylamine (TEA) (>99%), ammonium persulfate (APS) (>98%), and hydrochloric acid (37%) were all purchased from VWR (Darmstadt, Germany) whereas 2,4-toluene diisocyanate (2,4-TDI) (>98%) was purchased from TCI Chemicals (Eschborn, Germany) and was stored in the fridge in sealed bottles. DMSO, TEA, and toluene were stored over an A4 molecular sieve, and Acetone was stored over an A3 molecular sieve. Both molecular sieves were purchased from Carl Roth (Karlsruhe, Germany) and regenerated before use.

Abushammala H, & Mao J. (2023). Novel Electrically Conductive Cellulose Nanocrystals with a Core-Shell Nanostructure Towards Biodegradable Electronics. Nanomaterials, 13(4), 782.

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Thermoresponsive Polymer Hydrogel Synthesis

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All chemicals were obtained from Sigma-Aldrich unless otherwise described. N-Isopropylacrylamide (NIPAAm, TCI) was recrystallized in hexane 3 times before use. 2-Hydroxyethyl methacrylate (HEMA, Alfa Aesar) was used after passing through a column filled with inhibitor removers. Benzoyl peroxide (BPO, Fisher Scientific) was freeze-dried before polymerization. Acryloyl chloride (Sigma), 4-(hydroxymethyl)-phenylboronic acid pinacol ester (Sigma-Aldrich, HPPE), triethylamine (TEA, VWR), and hydrogen peroxide (H₂O₂) (30%, Sigma-Aldrich) were used as received. rhMG53 protein was purified from E. coli fermentation as described previously [33 ].

Niu H., Li H., Guan Y., Zhou X., Li Z., Zhao S.L., Chen P., Tan T., Zhu H., Bergdall V., Xu X., Ma J, & Guan J. (2022). Sustained delivery of rhMG53 promotes diabetic wound healing and hair follicle development. Bioactive Materials, 18, 104-115.

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Synthesis of Fluorinated Tryptophan Derivatives

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Racemic and L-Trp were purchased from Sigma-Aldrich (St. Louis, MO, USA), while racemic and L-5-fluorotryptophan (5-FTrp) and 6-fluorotryptophan (6-FTrp) were obtained from Apollo Scientific (Whitefield Rd, Bradbury, Stockport, UK). L-4,5-difluorotryptophan (L-4,5-diFTrp), L-4,6-difluorotryptophan (L-4,6-diFTrp), L-5,6-difluorotryptophan (L-5,6-diFTrp), L-5,7-difluorotryptophan (L-5,7-diFTrp) and L-4,5,6,7-tetrafluorotryptophan (L-4,5,6,7-tetraFTrp) were synthesized by the enzyme-catalyzed reaction of fluorinated indoles (4,5-di-, 4,6-di-, 5,6-di-, 5,7-di-, 4,5,6,7-tetra-fluoroindole) and L-serine, as reported in the literature [49 (link),50 (link),51 (link),52 (link)] and described in the Supporting Material.
For the synthesis of racemic 4,5-diFTrp, 4,6-diFTrp, 5,6-diFTrp, L-5,7-diFTrp, and 4,5,6,7-tetraFTrp, enzymatic racemization was carried out according to the method described in the literature [53 (link)].
Water, methanol (MeOH), acetonitrile (MeCN), formic acid (FA), and glacial acetic acid (AcOH) of LC-MS grade, ammonium formate (NH₄HCOO), ammonium acetate (NH₄OAc), and triethylamine (TEA) of analytical reagent grade were obtained from VWR International (Radnor, PA, USA).

Tanács D., Berkecz R., Bozsó Z., Tóth G.K., Armstrong D.W., Péter A, & Ilisz I. (2024). Liquid Chromatographic Enantioseparation of Newly Synthesized Fluorinated Tryptophan Analogs Applying Macrocyclic Glycopeptides-Based Chiral Stationary Phases Utilizing Core-Shell Particles. International Journal of Molecular Sciences, 25(9), 4719.

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Multifunctional Bioactive Chitosan Hydrogel

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Poly(ε-caprolactone) diol (PCL, Mn 2000 Da, Sigma-Aldrich, St. Louis, MO, USA), isophorone diisocyanate (IPDI, Acros, Geel, Belgium), Sn(Oct)₂ (Alfa Aesar, Haverhill, MA, USA), 2,2-bis(hydroxymethyl) propionic acid (DMPA, Sigma-Aldrich, St. Louis, MO, USA), triethylamine (TEA, J.T. Baker, Phillipsburg, NJ, USA), ethylenediamine (EDA, Tedia, Fairfield, OH, USA), glyoxal (Alfa Aesar, Haverhill, MA, USA), chitosan (170 kDa, 97% deacetylation, Kiotec, Hsinchu, Taiwan), acrylic acid (Showa, Gyoda, Japan), NaOH (Sigma-Aldrich, St. Louis, MO, USA), methacrylic anhydride (MAA, Sigma-Aldrich, St. Louis, MO, USA), acetic acid (Fluka, Buchs, Switzerland), pyrrole (Alfa Aesar, Haverhill, MA, USA), oxidant ammonium persulfate (APS, Sigma-Aldrich, St. Louis, MO, USA), HCl (Showa, Gyoda, Japan), high glucose Dulbecco’s modified Eagle’s medium (HG-DMEM, Gibco, Waltham, MA, USA), fetal bovine serum (FBS, Gibco, Waltham, MA, USA), Ham’s F-12 (Gibco, Waltham, MA, USA), G418 (Invitrogen, Waltham, MA, USA), penicillin-streptomycin-amphotericin (PSA, Caisson Laboratories, Inc., Smithfield, UT, USA), cell counting Kit-8 (CCK-8, Sigma-Aldrich, St. Louis, MO, USA), and KAPA SYBR Green qPCR kit (Kapa Biosystems, Inc., Wilmington, MA, USA) were applied in commercial form.

Xu J., Fu C.Y., Tsai Y.L., Wong C.W, & Hsu S.H. (2021). Thermoresponsive and Conductive Chitosan-Polyurethane Biocompatible Thin Films with Potential Coating Application. Polymers, 13(3), 326.

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Liposomal Ciprofloxacin Formulation and Characterization

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Liposomes containing 50 mg/ml ciprofloxacin (expressed in terms of ciprofloxacin hydrochloride) in a pH 6.0 histidine buffer were manufactured by Northern Lipids Inc. (Burnaby, BC, Canada) and Sigma-Tau (Indianapolis, IN). The following materials were used for the preparation, characterization or analysis of the liposomal ciprofloxacin (CFI) formulations: sucrose (Sigma-Aldrich (St. Louis, MO)), HEPES, free acid (Avantor (Center Valley, PA)), sodium chloride (Amresco (Solon, OH)), HPLC grade methanol (Fisher Scientific (Fair Lawn, NJ)), triethylamine (TEA, JT Baker (USA)), polysorbate 20 (VWR Int. (West Chester, NJ)), polyethylene glycol dodecyl ether (Brij 30 (Sigma-Aldrich (Australia))), Donor Adult Bovine Serum (HyClone (Logan, Utah)), and Nanosep centrifugal filtration devices, 10 K and 30 K molecular weight (Pall Corporation (Ann Arbor, MI)). Deionized water was used for all studies.

Cipolla D., Wu H., Eastman S., Redelmeier T., Gonda I, & Chan H.K. (2016). Tuning Ciprofloxacin Release Profiles from Liposomally Encapsulated Nanocrystalline Drug. Pharmaceutical Research, 33(11), 2748-2762.

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