Panc-1 and BxPC-3 cells were seeded in 6-well plates at 3×105 cells/well. After incubation for 24 h, ZJP was added to some wells (Panc-1 cells were treated with 200, 400 and 600 μg/mL ZJP; BxPC-3 cells were treated with 200, 400 and 600 μg/mL ZJP) for 48 h. Cell apoptosis was measured by flow cytometry using an annexin V-fluorescein isothiocyanate/propidium iodide (annexin V-FITC/PI) apoptosis detection kit (Genview, USA) according to the manufacturer’s instructions. The cells were collected and washed twice with cold phosphate-buffered saline, resuspended in 500 µL 1× binding buffer to which 5 µL annexin V-FITC and 5 µL PI staining solution had been added, and incubated for 15 min in the dark at room temperature. The sample was then mixed with an addition 500 µL of 1× binding buffer. Finally, cell apoptosis was measured by flow cytometry (BD Bioscience, USA), and the cytometric data were analyzed using FlowJo 7.6 software (De Novo Software, Los Angeles, CA, USA).
Annexin 5 fitc pi apoptosis detection kit
Manufactured by Genview
Sourced in United States, China
The Annexin V-FITC/PI Apoptosis Detection Kit is a laboratory tool used to detect and quantify apoptosis, a type of programmed cell death. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and propidium iodide (PI), a fluorescent dye that stains DNA. This combination allows for the identification of cells in different stages of apoptosis through flow cytometry analysis.
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4 protocols using annexin 5 fitc pi apoptosis detection kit
1
Apoptosis in Pancreatic Cancer Cells
2
Apoptosis Detection by Annexin V-FITC/PI Assay
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The drug-induced apoptosis was detected using an Annexin V-FITC/PI Apoptosis Detection Kit (Genview) according to the manufacturer’s instructions. Briefly, after incubation with indicated concentrations of drugs for 48 h, the cells were digested with 0.25% trypsin without EDTA, harvested with low-speed centrifugation, washed with PBS, and incubated with 5 μl Annexin V-FITC and 5 μl PI in 500 μl binding buffer for 10 min in the dark at room temperature. The stained cells were analyzed using a BD FACSAriaIII flow cytometer (BD Biosciences).
3
Quantifying Lung Cancer Cell Apoptosis
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To quantitively detect the apoptosis rate of lung cancer cells upon treatment with BHGJT, a flow cytometry assay was performed using an Annexin V-FITC/PI apoptosis detection kit (Genview, China). Cells in each group were treated as indicated. Then, the cells were collected, washed with PBS, resuspended in 100 μL binding buffer containing 5 μL Annexin V-FITC and 10 μL PI staining solution, and incubated in the dark at room temperature for 10 minutes. Then, 400 μL binding buffer was added to each sample and fully mixed. The apoptosis rate was analyzed by flow cytometry (Becton Dickinson, America).
4
Assessing Glioma Cell Apoptosis
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To evaluate the potential cell apoptosis effect of An-BMP-TMZ on glioma cells, U87 and U251 cells were seeded into 6-well plates and treated with free TMZ, BMP-TMZ or An-BMP-TMZ (TMZ concentration: 200 μM) for 48 h. After treatment, the cells were stained using an Annexin V-FITC/PI Apoptosis Detection Kit (GENVIEW) following the manufacturer's instructions. Flow cytometry was used to determine the results.
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