Vector red kit

Manufactured by Vector Laboratories

Sourced in Canada

The Vector® Red kit is a laboratory reagent used to detect and visualize target proteins or other biomolecules in biological samples. It provides a convenient and reliable method for signal amplification and color development.

Automatically generated - may contain errors

Lab products found in correlation

Alkaline phosphatase conjugated streptavidin, by Vector Laboratories (3 mentions) Dab kit, by Vector Laboratories (1 mentions) M o m kit, by Vector Laboratories (1 mentions) Imager a2 microscope, by Zeiss (1 mentions) Pecam1 antibodies, by Santa Cruz Biotechnology (1 mentions) Ko dmem, by Thermo Fisher Scientific (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Gelatin, by Merck Group (1 mentions) Doxycycline hyclate, by Merck Group (1 mentions) β mercaptoethanol, by Merck Group (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Methyl green, by Agilent Technologies (1 mentions) Methyl green, by Vector Laboratories (1 mentions) Mouse anti cd68 antibody, by Bio-Rad (1 mentions)

Spelling variants of this product

Vector Red Alkaline Phosphatase Substrate Kit (39 citations) Vector Red (36 citations) Vector Red Alkaline Phosphatase Substrate Kit I (15 citations) VECTOR Red AP Substrate Kit (10 citations) Vector Nova Red kit (7 citations) VECTOR® Red Alkaline Phosphatase (Red AP) Substrate Kit (3 citations) Vector Red alkaline phosphatase staining kit (3 citations) Vector Red AP Substrate Kit I (3 citations) ImmPACT Vector Red AP Substrate Kit (2 citations) Vector Red Alkaline Phosphate Substrate Kit (2 citations)

6 protocols using vector red kit

Assessing Ameloblast Barrier Function

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The apical tight junction is an important indicator for ameloblast polarity and can be measured by the functional selectivity of the paracellular apical barrier. Sulfo-NHS-Biotin was used as a tracer molecule to evaluate the effects of SATB1 on the ameloblast layer barrier function as previously described [78 (link)]. Briefly, four 10-day old pups from each wt and Satb1^−/− mouse model were anesthetized by intraperitoneal injection with 0.05 ml/10 g of ketamine (18 mg/ml). A 30-G injection needle with a blunt end was carefully inserted into the left ventricle of the heart, and 150 μl/g of Sulfo-NHS-Biotin solution was perfused into the bloodstream. Ten minutes after receiving Sulfo-NHS-Biotin injection, the pups were sacrificed, and the hemimandibles were dissected, processed, and paraffin-embedded for histological sectioning as previously mentioned. The sagittal sections were blocked with 5% BSA for 1 h then incubated with alkaline phosphatase-conjugated streptavidin (Vector Laboratories, Inc.) for 30 min, and immunoreactivity was visualized using a Vector® Red kit (Vector Laboratories, Inc.).

Zhang Y., Zheng L., Le M., Nakano Y., Chan B., Huang Y., Torbaty P.M., Kohwi Y., Marcucio R., Habelitz S., Den Besten P.K, & Kohwi-Shigematsu T. (2019). SATB1 establishes ameloblast cell polarity and regulates directional amelogenin secretion for enamel formation. BMC Biology, 17, 104.

+ Open protocol

+ Expand

Immunostaining of NFATC1 and NOTCH1

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue sections were prepared as described above for histology and then antigen retrieved by boiling for 10 min in sodium citrate (10 mM, pH 6.0) (Vector Laboratories). The tissues were incubated in 1% H2O2 for 30 min to quench the endogenous hydroperoxide activities. M.O.M kit (Vector Laboratories) was used for immunostaining for NFATC1 antibody (BD Pharmingen, 556,602). The brown color was developed using DAB kit (Vector Laboratories). The tissues were then blocked with 5% normal horse serum in PBS before being incubated with PECAM1 antibodies (Santa Cruz Biotechnology, sc-1506) and secondary anti-bodies. The red color was developed using Vector Red Kit (Vector Laboratories). Immunofluorescence staining of NOTCH1 was performed on frozen sections. The embryos were collected at E10.5 and fixed in 4%PFA at 4 °C for one hour, soak in 15% and 30% sucrose sequentially and embedded in OCT compounds with orientation for front sections at 8 μm on the positive charged slides. Tissue sections were then post-fixed in the cold ethanol and acetone (1:1) solution for 5 min and stored at − 80 °C. Tissue sections were air dried for 45 min before staining. The tissues were then blocked with 5% normal horse serum in PBS before being incubated with NOTCH1 antibodies (R&D, AF5267) and secondary antibodies. Stained tissue sections were photographed using a Zeiss Imager A2 microscope.

Wang Y., Lu P., Wu B., Morrow B.E, & Zhou B. (2018). NOTCH maintains developmental cardiac gene network through WNT5A. Journal of molecular and cellular cardiology, 125, 98-105.

+ Open protocol

+ Expand

Induction of Pluripotency in Reprogrammable MEFs

Check if the same lab product or an alternative is used in the 5 most similar protocols

To induce expression of the pluripotency factors, reprogrammable MEFs were seeded at a density of 15,000 cells per well of a 12 well plate coated with 0.2% gelatin (Sigma). Induction media contained ES culture media (500 ml KO-DMEM (Life Technologies) 15% fetal bovine serum (Hyclone), 1X non-essential amino acids (Life Technologies), 1X Glutamax (Life Technologies), 1000 U/ml leukemia inhibitory factor, 55 μM beta-mercaptoethanol (Sigma)) supplemented with 50 μg/ml ascorbic acid (Sigma) and 2 μg/ml doxycycline hyclate (Sigma). Unless otherwise indicated, reprogrammable MEFs were induced for 12 days, followed by 4 days of doxycycline withdrawal to ensure transgene independence. Alkaline phosphatase staining was performed using a Vector Red kit (Vector Labs) according to the manufacturer’s recommendation.

Brumbaugh J., Di Stefano B., Wang X., Borkent M., Forouzmand E., Clowers K.J., Ji F., Schwarz B.A., Kalocsay M., Elledge S.J., Chen Y., Sadreyev R.I., Gygi S.P., Hu G., Shi Y, & Hochedlinger K. (2017). Nudt21 controls cell fate by connecting alternative polyadenylation to chromatin signaling. Cell, 172(1-2), 106-120.e21.

+ Open protocol

+ Expand

Mandible Immunohistochemistry for AR, TGFBR2, TGFB1, PR

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse mandibles were fixed in 4% paraformaldehyde in 0.06 M sodium cacodylate buffer (pH 7.3) at 4°C for 24 h. After decalcification in 8% EDTA (pH 7.3), samples were processed for routine paraffin embedding and sagittally sectioned. The sections were incubated with 10% swine and 5% goat sera followed by incubation with rabbit anti-human AR (1:75; Novus Biologicals, Littleton, CO, NB100-91658), rabbit anti-mouse TGFBR2 (1:100; Santa Cruz Biotech, Santa Cruz, CA, sc-1700), rabbit anti-human TGFB1 (1:50; Abcam PLC, Cambridge, MA, ab92486), and rabbit anti-mouse PR (Santa Cruz Biotech, sc-166170) antibodies respectively overnight at room temperature. A biotinylated swine anti-rabbit IgG F(ab')2 fraction (Dako, Carpinteria, CA) was used as the secondary antibody for 1 h at room temperature incubation. Following incubation with alkaline phosphatase conjugated streptavidin (Vector Laboratories Inc., Burlingame, CA) for 30 min, immunoreactivity was visualized using a Vector® Red kit (Vector Laboratories) resulting in pink/red color for positive staining. Counter-staining was performed with methyl green (Dako). Negative control was done with normal rabbit sera.

Le M.H., Nakano Y., Abduweli Uyghurturk D., Zhu L, & Den Besten P.K. (2017). Fluoride Alters Klk4 Expression in Maturation Ameloblasts through Androgen and Progesterone Receptor Signaling. Frontiers in Physiology, 8, 925.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Mandible

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dissected mandibles were immediately immersed into 4% PFA for 1 day at 4°C followed by decalcification in 8% EDTA (pH 7.3) at 4°C for 4 weeks. The mandibles were then processed through a graded series of ethanol and xylene followed by routine embedding in paraffin and sectioning. The sections were deparaffinized, and blocked for nonspecific staining with 10% swine and 5% goat sera for 1 hour, followed by incubation with rabbit anti-mouse SATB1 antibody (Epitomics, Burlingame, CA) or rabbit anti-phosphorylated PKCα antibody (Abcam, Cambridge, MA) overnight at 4°C. The sections were next thoroughly washed with 0.2% Tween/PBS, and incubated with a biotin-conjugated swine anti-rabbit IgG F(ab’)2 fraction (Dako Cytomation Inc., Carpinteria, CA) for 1 hour at room temperature. The sections were then incubated with alkaline phosphatase conjugated streptavidin (Vector Laboratories Inc., Burlingame, CA) for 30 minutes, and immunoreactivity was visualized using a Vector Red kit (Vector Laboratories Inc.) resulting in pink/red color for positive staining. Counter-staining was done with methyl green (Vector Laboratories Inc). The sections were photographed with a Nikon Eclipse 300 microscope (Compix Inc, Sewickley, PA).

Zhang Y., Kim J.Y., Horst O., Nakano Y., Zhu L., Radlanski R.J., Ho S, & Besten P.K. (2014). Fluorosed Mouse Ameloblasts Have Increased SATB1 Retention and Gαq Activity. PLoS ONE, 9(8), e103994.

+ Open protocol

+ Expand

Immunohistochemical Staining of CD68+ Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue sections were first fixed in ice-cold acetone for 10 min. After washing with PBS, they were incubated in a solution of 4% rabbit serum for 10 min to block non-specific binding of the CD68 antibody, and then incubated with 1:200 dilution of primary antibody (mouse anti-CD68 antibody; Serotec, NC) for 1 h. Sections were rinsed 3 times in PBS and incubated for 10 minutes with a biotinylated rat anti-mouse secondary antibody. Tissue was rinsed 3X in PBS and then incubated for 5 min in an alkaline phosphatase-conjugated streptavidin solution. Finally, staining was developed using the Vector Red Kit (Vector Laboratories). Staining was stopped by dipping the slides into distilled water. Slides were then dehydrated by sequential immersion into 70%, 80%, 90%, and 100% ethanol, stained with hemaetoxylin for 1 min, and then cleared with xylene for 3 min and mounted in a resinous medium. These CD68+ stained sections were later used as “guide slides” during laser capture microdissection (LCM) in order to assure that all laser-captured material was specific for CD68+ cells (which were mainly monocyte-derived macrophages and foam cells).

Willecke F., Yuan C., Oka K., Chan L., Hu Y., Barnhart S., Bornfeldt K.E., Goldberg I.J, & Fisher E.A. (2015). Effects of High Fat Feeding and Diabetes on Regression of Atherosclerosis Induced by Low-Density Lipoprotein Receptor Gene Therapy in LDL Receptor-Deficient Mice. PLoS ONE, 10(6), e0128996.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!