Collagen 2

Manufactured by Boster Bio

Sourced in China

Collagen II is a type of collagen protein that is a major structural component of articular cartilage. It provides tensile strength and support to the cartilage tissue.

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Lab products found in correlation

Phenylmethanesulfonyl fluoride, by Solarbio (1 mentions) Phosphatase inhibitor cocktail, by Selleck Chemicals (1 mentions) Mmp13, by Abcam (1 mentions) Ncoa4, by Cell Signaling Technology (1 mentions) Mmp 3, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Ripa lysis buffer, by Solarbio (1 mentions) Cox 2, by Proteintech (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Cell Signaling Technology (1 mentions) Adamts5, by Cell Signaling Technology (1 mentions) β catenin, by Cell Signaling Technology (1 mentions) Bcl2 associated x (bax), by Boster Bio (1 mentions) Mmp 13, by Boster Bio (1 mentions) Bcl 2, by Boster Bio (1 mentions) Caspase 3, by Boster Bio (1 mentions) Beclin 1, by Boster Bio (1 mentions) β actin, by Boster Bio (1 mentions) Radioimmunoprecipitation assay (ripa), by Beyotime (1 mentions)

3 protocols using collagen 2

Protein Extraction and Western Blot Analysis of Human Cartilage

Cited 1 time

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The total protein from human cartilages and chondrocytes with various treatment were extracted using RIPA lysis buffer (#R0010, Solarbio) containing 1 mM phosphatase inhibitor cocktail (#B15002, Bimake, USA) and 1 mM phenylmethanesulfonyl fluoride (#329-98-6, Solarbio). Western blot was performed essentially as previously described.¹⁸ (link) The primary antibodies used were: NCOA4 (#66849, Cell Signaling Technology), Tf (#17435-1-AP, Proteintech, RRID:AB_2035023), COX2 (#66351-1-Ig, Proteintech, RRID:AB_2881731), MMP3 (#14351, Cell Signaling Technology, RRID:AB_2798459), Collagen II (#BA0533, Boster), FTH1 (#4393, Cell Signaling Technology, RRID:AB_11217441), MMP13 (#ab219620, Abcam), TRPV1 (#66983-1-Ig, Proteintech, RRID:AB_2882302), GPX4 (#ab125066, Abcam, RRID:AB_10973901) and GAPDH (#5174, Cell Signaling Technology, RRID:AB_10622025) (Supplementary Table 4). After horseradish peroxidase-conjugated goat anti-rabbit/mouse secondary antibodies (#BL003A or #BL001A, Biosharp) incubation, western blots were imaged by the ChemiDocXRS + Imaging System (Tanon, Shanghai, China). Image J (version 1.8.0) was used for the quantitative analysis of proteins. All antibodies used in this study were commercial antibodies.

Lv Z., Han J., Li J., Guo H., Fei Y., Sun Z., Dong J., Wang M., Fan C., Li W., Xie Y., Sun W., Chen J., Liu Y., Chen F., Liu Z., Liu A., Wu R., Xu X., Yan W., Jiang Q., Ikegawa S., Chen X, & Shi D. (2022). Single cell RNA-seq analysis identifies ferroptotic chondrocyte cluster and reveals TRPV1 as an anti-ferroptotic target in osteoarthritis. eBioMedicine, 84, 104258.

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Immunohistochemical Analysis of Mouse Knee Joint

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After deparaffinization and hydration, the histological slides of the mouse knee joint were subjected to antigen retrieval and blocked with 5% bovine serum albumin (BSA) for 1 h at room temperature. Following incubation overnight (4 °C) with primary antibodies against collagen II (#BA0533, Boster, Wuhan, China), ADAMTS5 (#14351, Cell Signaling Technology, USA), MMP13 (#ab219620, Abcam, UK), FRZB (#ab205284, Abcam, UK), and β-catenin (#8814S, Cell Signaling Technology), the slides were incubated with a horseradish peroxidase-conjugated secondary antibody (Biosharp, Shanghai, China) for 30 min at 37 °C. An ultrasensitive DAB Kit (Typing, Nanjing, China) was used to visualize the immunohistochemical staining.

An X., Wang R., Lv Z., Wu W., Sun Z., Wu R., Yan W., Jiang Q, & Xu X. (2024). WTAP-mediated m6A modification of FRZB triggers the inflammatory response via the Wnt signaling pathway in osteoarthritis. Experimental & Molecular Medicine, 56(1), 156-167.

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Chondrocyte Protein Analysis via Western Blot

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RIPA (Beyotime, China) was used to harvest total protein of chondrocytes from cell lysates. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was applied for separating protein, and then protein was transferred to polyvinylidene difluoride (PVDF) membrane. Primary antibodies incubated for 24h, and then secondary antibody incubated in IgG conjugated horseradish peroxidase (HRP) for another 2h. Enhanced chemiluminescence (ECL) kit was applied to detect light-emitting signal. The primary antibodies were used for ATG2B, Beclin 1, LC3, p62, Collagen II, MMP13, CHOP, p-eIF2a, Bax, Bcl-2, Caspase 3, and β-actin (BOSTER, China).

Li H., Li Z., Pi Y., Chen Y., Mei L., Luo Y., Xie J, & Mao X. (2020). MicroRNA-375 exacerbates knee osteoarthritis through repressing chondrocyte autophagy by targeting ATG2B. Aging (Albany NY), 12(8), 7248-7261.

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