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Tecnai g2 twin transmission electron microscope

Manufactured by Thermo Fisher Scientific
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The Tecnai G2 TWIN transmission electron microscope is a high-performance imaging and analysis tool designed for a wide range of applications. It provides high-resolution imaging capabilities and advanced analytical features to support scientific research and investigations. The core function of this instrument is to enable the examination and analysis of samples at the nano-scale level.

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Lab products found in correlation

Em uc7, by Leica (2 mentions) Metal grids, by Electron Microscopy Sciences (2 mentions) Whatman filter paper, by Cytiva (2 mentions) Glutaraldehyde, by Merck Group (2 mentions) Uv 3600, by Shimadzu (1 mentions) Titan 80 300, by Thermo Fisher Scientific (1 mentions) Fluostar omega, by BMG Labtech (1 mentions)

Close spelling variants of this product

Tecnai T12 G2 TWIN transmission electron microscope Tecnai G2 Spirit Twin Bio-Transmission Electron Microscope Tecnai G2 F20 X-TWIN transmission electron microscope Tecnai G2 sprit Twin Transmission Electron Microscope FEI Tecnai G2 F20 S-TWIN transmission electron microscope Tecnai G2 F20-TWIN transmission electron microscope Tecnai G2 Spirit TWIN/BioTWIN transmission electron microscope Tecnai G2 Spirit TWIN transmission electron microscope Tecnai G2 Spirit TWIN 120 kV transmission electron microscope Tecnai G2 S-Twin transmission electron microscope

5 protocols using tecnai g2 twin transmission electron microscope

1

Glutaraldehyde and Osmium Fixation Protocol

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In all, 2.5% glutaraldehyde (Sigma-Aldrich, USA) was used to fix cell samples for 1 h, and then fixed for 2 h in 2% osmium tetraoxide, washed with water, and stained with 0.5% uranyl acetate for 12 h. After dehydration and polymerization, samples were cut as ultrathin sections of 70–90 nm with an ultramicrotome, (EM UC7, Leica), and then a Tecnai G2 TWIN transmission electron microscope (FEI, USA) was used to view and capture images.
Li G., Song Y., Liao Z., Wang K., Luo R., Lu S., Zhao K., Feng X., Liang H., Ma L., Wang B., Ke W., Yin H., Zhan S., Li S., Wu X., Zhang Y, & Yang C. (2020). Bone-derived mesenchymal stem cells alleviate compression-induced apoptosis of nucleus pulposus cells by N6 methyladenosine of autophagy. Cell Death & Disease, 11(2), 103.
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2

Ultrathin Specimen Preparation for TEM

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NP cell samples were fixed in 2.5% glutaraldehyde (Sigma‒Aldrich, USA) for 1 h followed by 2% osmium tetraoxide for 2 h. After washing with water, the cell samples were stained with 0.5% uranyl acetate for 12 h, followed by dehydration and polymerization. Ultrathin sections were cut at thicknesses of 70–90 nm with an ultramicrotome (EM UC7, Leica) and imaged with a Tecnai G2 TWIN transmission electron microscope (FEI, USA).
Song Y., Liang H., Li G., Ma L., Zhu D., Zhang W., Tong B., Li S., Gao Y., Wu X., Zhang Y., Feng X., Wang K, & Yang C. (2023). The NLRX1-SLC39A7 complex orchestrates mitochondrial dynamics and mitophagy to rejuvenate intervertebral disc by modulating mitochondrial Zn2+ trafficking. Autophagy, 20(4), 809-829.
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3

Characterization of Functionalized Gold Nanoparticles

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The absorbance spectra of the purified
GNS (both PEGylated and bare) suspended in Milli-Q water were recorded
using a multimode microplate reader, FLUOstar Omega (BMG Labtech),
and a UV-3600 (Shimadzu) spectrophotometer. The morphology and the
particle size distribution of Au NPs were evaluated by using FEI Tecnai
G2 Twin transmission electron microscope, and HAADF STEM
images and EDX maps were acquired using FEI Titan 80-300. Hydrodynamic
particle size distribution and nanoparticle concentration were measured
by nanoparticle-tracking analysis using a NanoSight NS500 instrument
(Malvern, Worcestershire, U.K.).
De Silva Indrasekara A.S., Johnson S.F., Odion R.A, & Vo-Dinh T. (2018). Manipulation of the Geometry and Modulation of the Optical Response of Surfactant-Free Gold Nanostars: A Systematic Bottom-Up Synthesis. ACS Omega, 3(2), 2202-2210.
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4

Transmission Electron Microscopy Sample Preparation

Cited 1 time
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As previously published with some modifications (Manning and Kuehn, 2011 (link)), to prepare samples for TEM imaging, metal grids (Electron Microscopy Sciences; 300 mesh copper, formvar/carbon) were cleaned using the Pelco EasiGlow Machine Vacuum and then 10 μL of sample was added. Samples were pipetted onto the grid and let set for 1 min before wicking away excess with a Whatman filter paper. The samples were stained by applying 10 μL of 2% uranyl acetate, incubated 1.5 min, and excess uranyl acetate removed by wicking with Whatman filter paper. Grids were stored in Petri dishes at room temperature. Samples were imaged using a FEI Tecnai G2 Twin Transmission Electron Microscope with a spot size of 2 at a voltage of 120kV.
McMillan H.M., Zebell S.G., Ristaino J.B., Dong X, & Kuehn M.J. (2021). Protective plant immune responses are elicited by bacterial outer membrane vesicles. Cell reports, 34(3), 108645.
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5

Transmission Electron Microscopy Sample Preparation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
As previously published with some modifications (Manning and Kuehn, 2011 (link)), to prepare samples for TEM imaging, metal grids (Electron Microscopy Sciences; 300 mesh copper, formvar/carbon) were cleaned using the Pelco EasiGlow Machine Vacuum and then 10 μL of sample was added. Samples were pipetted onto the grid and let set for 1 min before wicking away excess with a Whatman filter paper. The samples were stained by applying 10 μL of 2% uranyl acetate, incubated 1.5 min, and excess uranyl acetate removed by wicking with Whatman filter paper. Grids were stored in Petri dishes at room temperature. Samples were imaged using a FEI Tecnai G2 Twin Transmission Electron Microscope with a spot size of 2 at a voltage of 120kV.
McMillan H.M., Zebell S.G., Ristaino J.B., Dong X, & Kuehn M.J. (2021). Protective plant immune responses are elicited by bacterial outer membrane vesicles. Cell reports, 34(3), 108645.
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