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Rabbit anti nmiia

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Rabbit anti-NMIIA is a primary antibody that specifically recognizes non-muscle myosin IIA (NMIIA), a protein involved in cellular processes such as cell division, cell migration, and intracellular transport. This antibody can be used for applications like Western blotting, immunohistochemistry, and immunocytochemistry to detect and study the expression and localization of NMIIA in various cell and tissue types.

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Lab products found in correlation

Rabbit anti actin, by Abcam (1 mentions) Rabbit anti β tubulin, by Abcam (1 mentions)

2 protocols using rabbit anti nmiia

1

Quantitative Western Blot Analysis

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20 µg cell lysates were subjected to SDS–PAGE and electrically blotted on to PVDF membrane. After blocking with 2% (w/v) BSA, the blot was probed with rabbit anti-β-tubulin (Abcam), rabbit anti-actin (Abcam), rabbit anti-NMIIA (Abcam) or rabbit anti-S100P (R&D Systems). After extensive washing, anti-rabbit-HRP (Santa Cruz) 1/10,000 was used and the bands were visualised using ECL and their densities were measured using Image Lab software.
Du M., Wang G., Barsukov I.L., Gross S.R., Smith R, & Rudland P.S. (2020). Direct interaction of metastasis-inducing S100P protein with tubulin causes enhanced cell migration without changes in cell adhesion. Biochemical Journal, 477(6), 1159-1178.
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2

Angiotensin Receptor Expression in Fibroblasts

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HSF and fibroblasts from unwounded human tissue were used to determine AT1-receptor and AT2-receptor expression. Primary antibodies used were rabbit anti-AT1-receptor (1μg/ml Santa Cruz Biotechnology), rabbit anti-AT2-receptor (1μg/ml LifeSpan BioSciences, Inc., Seattle, WA). 3T3 fibroblasts incubated in 0.1μM AngII for 4d prior to experiment were used to determine NM IIA, Vimentin, ASMA, and ROCK expression. Primary antibodies used were rabbit anti-NM IIA (1:1000 dilution Abcam, Cambridge, MA), goat anti-vimentin (1:200 dilution, eBioscience), rabbit anti-ASMA (1:200 dilution Abcam), rabbit anti-ROCK (1:200 dilution, Abcam). WB analysis was performed as described by Bond et al [22 (link)].
Ehanire T., Ren L., Bond J., Medina M., Li G., Bashirov L., Chen L., Kokosis G., Ibrahim M., Selim A., Blobe G.C, & Levinson H. (2014). Angiotensin II Stimulates Canonical TGFβ Signaling Pathway through Angiotensin Type 1 Receptor to Induce Granulation Tissue Contraction. Journal of molecular medicine (Berlin, Germany), 93(3), 289-302.
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