For analysis of liver lipid and glucose content, 20 mg of frozen liver was homogenized in 400 μl of 50 mM Tris + 5% Triton-X 100, then samples were heated to 80–100 °C and cooled to room temperature (repeated twice). After centrifugation, the supernatants were diluted 1–5 fold, lipids were quantified using Wako Clinical Diagnostics kits (Wako USA, Richmond, VA), and glucose concentrations assayed using a Glucometer (Bayer, Pittsburgh, PA).
Wako clinical diagnostics kits
Manufactured by Fujifilm
Sourced in United States
Wako Clinical Diagnostics kits are a range of laboratory equipment produced by Fujifilm. These kits are designed for clinical diagnostic testing and analysis. The core function of the Wako Clinical Diagnostics kits is to provide accurate and reliable results for various medical tests and assessments.
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2 protocols using wako clinical diagnostics kits
1
Quantifying Liver Lipids and Glucose
2
Biomarker Profiling of Metabolic Status
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Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were assessed in a 96-well microplate using a commercial ALT or AST assay kit (Catachem, Bridgeport, CT), and monitored at 340 nm for 5 min with a microplate reader (BioAssay Systems, Harvard, CA). Serum ketone bodies and non-esterified fatty acids (NEFA) were assayed using Wako Clinical Diagnostics kits (Wako USA, Richmond, VA). Serum insulin levels were tested with an ultra-sensitive mouse insulin ELISA kit (90080, Crystal Chem, Downers Grove, IL).
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