In situ hybridization was performed as described [26 ]. Anti-sense probes were generated as described by Kelley et al. [29 (link)]. Briefly, anti-sense probes were synthesized with digoxigenin-coupled UTP (Roche) and detected with alkaline phosphatase coupled to anti-digoxigenin Fab fragments (Roche) followed by the chromogenic reaction with NBT/BCIP (Vectstain).
Digoxigenin coupled utp
Manufactured by Roche
Digoxigenin-coupled UTP is a nucleotide analog used in molecular biology techniques. It serves as a labeling reagent, allowing the detection and visualization of target nucleic acid sequences through various hybridization-based methods.
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Lab products found in correlation
2 protocols using digoxigenin coupled utp
1
In situ Hybridization Protocol for RNA Detection
2
In situ Hybridization Protocol for RNA Detection
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