Thermalert thermometer

Manufactured by Physitemp

The Thermalert thermometer is a handheld device used to measure and display temperature. It features a digital display and utilizes infrared technology to provide accurate and non-contact temperature readings.

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Lab products found in correlation

Vt1000, by Leica (2 mentions) Ccd camera, by Hamamatsu Photonics (2 mentions) It 23, by Physitemp (2 mentions) Axioskop, by Zeiss (1 mentions)

Close spelling variants of this product

TH-8 Thermalert monitoring thermometer Thermalert clinical monitoring thermometer Thermalert TH-5 thermometer TH-5 Thermalert Monitoring Thermometer TH-5 Thermalert Clinical Monitoring Thermometer

2 protocols using thermalert thermometer

Cochlear Nuclei Slicing and Electrophysiology

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Coronal slices of the cochlear nuclei were made from mice between P17 and P25. Slices (220 µm thick) were cut with a vibrating microtome (Leica VT 1000S) in normal physiological saline or in saline with reduced Na⁺ at 24–27°C, and then transferred to a recording chamber (∼0.6 ml) and superfused continually at 5–6 ml/min. Temperature was controlled with a Thermalert thermometer (Physitemp) the input of which comes from a small thermistor (IT-23, Physitemp, diameter: 0.1 mm) placed between the inflow of the chamber and the tissue. The output of the Thermalert thermometer was fed into a custom-made, feedback-controlled heater that heated the saline in glass tubing (1.5 mm) just before it reached the chamber to maintain the temperature at 33°C. Biocytin injections were made under the control of a Wild (M5) dissecting microscope. For electrophysiological recordings, the tissue was visualized through a compound microscope (Zeiss Axioskop) with a 63× water immersion objective and CCD Camera (Hamamatsu), with the image displayed on a video screen.

Lu C.C., Cao X.J., Wright S., Ma L., Oertel D, & Goodrich L.V. (2014). Mutation of Npr2 Leads to Blurred Tonotopic Organization of Central Auditory Circuits in Mice. PLoS Genetics, 10(12), e1004823.

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Electrophysiological Recording of Ventral Cochlear Nucleus

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Coronal slices of the most caudal part of the VCN were made from mice between 16 and 22 days after birth. Slices, 210 μm thick, were cut with a vibrating microtome (Leica VT 1000S). Slices were maintained in a recording chamber (~0.6 ml) that was superfused with normal saline at 5 to 6 ml/min. The recording chamber was mounted on the stage of a compound microscope (Zeiss Axioskop) and viewed through a 63X water immersion objective whose image was displayed through a Hamamatsu CCD camera (C2400-77AH) on a video monitor. The temperature was measured between the inflow of the chamber and the tissue with a Thermalert thermometer (Physitemp) through a small thermistor (IT-23, Physitemp, diameter: 0.1 mm). The output of the Thermalert thermometer was fed into a custom-made, feedback-controlled heater that heated the saline in glass tubing (1.5 mm inner, 3 mm outer diameter) just before it reached the chamber to keep the temperature constant at 33°C. An adjustable delay in the controller for the heater prevented temperature oscillations. Recordings were generally made within two hours after slices were cut.

Cao X.J, & Oertel D. (2017). Genetic Perturbations Suggest a Role of the Resting Potential in Regulating the Expression of the Ion Channels of the KCNA and HCN families in Octopus Cells of the Ventral Cochlear Nucleus. Hearing research, 345, 57-68.

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