L(-) lactide (Polysciences Inc.) was recrystallized from Toluene before use. 2-Hydroxyethyl methacrylate (HEMA) (Aldrich, 97%) was dried over molecular sieves (4Ǻ) for 24 hours and distilled under negative pressure before use. Toluene (Acros, 99%) and pyridine (Sigma, ≥ 99%) were refluxed over calcium hydride for one hour and distilled prior to use. Methacryloyl chloride (Aldrich, 97%) was distilled prior to use. Stannous octoate (Sigma, 95%), phosphorous pentoxide (Aldrich, 97%), hydroxylamine hydrochloride reagentplus® (Sigma, 99%), chloroform (Certified A.C.S, Fisher Scientific), hydrochloric acid (Aldrich, 37%) were used as received. All other solvents were used as received. Paclitaxel from Taxus brevifolia was obtained from Sigma-Aldrich (St. Louis, MO, USA). All cell media, heat-inactivated fetal bovine serum (FBS), trypsin-EDTA, penicillin-streptomycin were obtained from Invitrogen (Carlsbad, CA, USA). Hoechst® 33342, CellMask™ deep red plasma membrane stain kit, Rhodamine-123 and Bodipy® 665/676 were obtained from Invitrogen. Phosphate-buffered saline (PBS) was from Mediatech (Manassas, VA, USA) and the CellTiter-Glo® Luminescent Cell Viability Assay Kit was obtained from Promega (Madison, WI, USA).
Paclitaxel from taxus brevifolia
Manufactured by Merck Group
Sourced in United States
Paclitaxel is a natural compound extracted from the bark of the Pacific yew tree (Taxus brevifolia). It is a complex diterpenoid molecule with a unique chemical structure that has been extensively studied for its pharmaceutical properties. Paclitaxel is primarily used as a reference standard and research tool in the field of biochemistry and molecular biology, particularly for its role in cellular processes involving microtubules.
Automatically generated - may contain errors
Lab products found in correlation
Toluene, by Thermo Fisher Scientific (1 mentions)
Rhodamine 123, by Thermo Fisher Scientific (1 mentions)
Heat inactivated fetal bovine serum, by Thermo Fisher Scientific (1 mentions)
Chloroform, by Thermo Fisher Scientific (1 mentions)
L lactide, by Polysciences (1 mentions)
Bodipy 665 676, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Hoechst 33342, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Celltiter glo luminescent cell viability assay kit, by Promega (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Pyridine, by Merck Group (1 mentions)
Stannous octoate, by Merck Group (1 mentions)
Methacryloyl chloride, by Merck Group (1 mentions)
2 hydroxyethyl methacrylate hema, by Merck Group (1 mentions)
Phosphorous pentoxide, by Merck Group (1 mentions)
Epidermal growth factor (egf), by Merck Group (1 mentions)
Gemcitabine hydrochloride, by Merck Group (1 mentions)
5 fluorouracil 5 fu, by Merck Group (1 mentions)
Cis diamineplatinum 2 dichloride, by Merck Group (1 mentions)
3 protocols using paclitaxel from taxus brevifolia
1
Synthesis and Characterization of Paclitaxel-Loaded Polymers
2
Preparation of Chemotherapeutic Agents
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EGF (Sigma-Aldrich, St Louis, MO, USA), the natural ligand of the EGFR, was reconstituted in sterile 1× phosphate-buffered saline (PBS) at a stock concentration of 1 mg/mL and stored at −20°C. EGF concentrations to stimulate cells were 30–100 ng/mL. Incubation times varied between experiments and thus are indicated.
cis-Diamineplatinum(II) dichloride (P4394; Sigma-Aldrich) was reconstituted in dimethyl sulfoxide (DMSO) to make a 27.7 mM stock solution. Gemcitabine hydrochloride (G6423; Sigma-Aldrich) was reconstituted in 1× PBS to make a 133.5 mM stock solution. 5-Fluorouracil (5-FU) (F6627; Sigma-Aldrich) was reconstituted in a mixture of 1 mL DMSO and 9 mL 1× PBS to make 2.31 mM 5-FU stock. Paclitaxel from Taxus brevifolia, (T7402, Sigma-Aldrich), was reconstituted in DMSO to make 1.17 mM stock. These stocks were further diluted in 1× DMEM conditioned medium to make various dosages of the chemotherapeutic agents to be used in in vitro experiments.
cis-Diamineplatinum(II) dichloride (P4394; Sigma-Aldrich) was reconstituted in dimethyl sulfoxide (DMSO) to make a 27.7 mM stock solution. Gemcitabine hydrochloride (G6423; Sigma-Aldrich) was reconstituted in 1× PBS to make a 133.5 mM stock solution. 5-Fluorouracil (5-FU) (F6627; Sigma-Aldrich) was reconstituted in a mixture of 1 mL DMSO and 9 mL 1× PBS to make 2.31 mM 5-FU stock. Paclitaxel from Taxus brevifolia, (T7402, Sigma-Aldrich), was reconstituted in DMSO to make 1.17 mM stock. These stocks were further diluted in 1× DMEM conditioned medium to make various dosages of the chemotherapeutic agents to be used in in vitro experiments.
3
Inhibition of BET Proteins and Chemotherapeutic Treatments
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For BET inhibition, cells were treated with 1 µM JQ1 (Sigma) or 1 µM IBET-151 (GSK1210151A, Selleckchem) dissolved as 10 mM in DMSO; DMSO was used as vehicle control. For treatment with chemotherapeutic drugs, 10 mM Doxorubicin hydrochloride (Sigma) dissolved in water and 1 mM Paclitaxel from Taxus brevifolia (Sigma) dissolved in DMSO were added to the cells’ media at the concentrations indicated in the figures. DMSO (vehicle control) was used at a concentration matching the maximum concentration of paclitaxel used in a given experiment.
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