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Granzyme b percp

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Granzyme B-PerCP is a flow cytometry reagent used for the detection and quantification of granzyme B-positive cells. Granzyme B is a serine protease enzyme involved in the process of apoptosis. The PerCP (Peridinin Chlorophyll Protein Complex) fluorophore is conjugated to the anti-granzyme B antibody, enabling its detection and measurement by flow cytometry.

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Lab products found in correlation

Cx3cr1 apc cy7, by BioLegend (5 mentions) Cd11a apc, by BioLegend (5 mentions) Cytoflex lx, by Beckman Coulter (5 mentions) Cd8 pe cy7, by BD (5 mentions) Pd 1 fitc, by BioLegend (4 mentions) Bim pe, by Cell Signaling Technology (4 mentions) Ki 67 bv421, by BD (2 mentions) Live dead fixable stain kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Granzyme B (2 citations)

5 protocols using granzyme b percp

1

Comprehensive Immune Profiling of PBMC Populations

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The following panel of antibodies was used for analysis of PBMC populations: CD8-PE-Cy7 (BD Pharmingen, clone RPA-T8, catalog 304006), CD11a-APC (BioLegend, clone HI111, catalog 301212), PD-1 FITC (BioLegend, clone EH12.2H7, catalog 32990), CX3CR1-APC/Cy7 (BioLegend, clone 2A9-1, catalog 341616), Bim-PE (Cell Signaling Technology, Danvers, Massachusett, clone C34C5, catalog 12186S), Ki-67-BV421(BD Biosciences, clone B56, catalog 562899), Granzyme B-PerCP (Novus Biologicals, Danvers, Massachusett, clone CLB-GB11, catalog NBP1-50071PCP). CD8+ T-cells were first stained for surface markers followed by intracellular staining. Flow cytometry data were collected on a CytoFLEX LX (Beckman Coulter, Atlanta, Georgia). Flow cytometry analysis was performed with FlowJo software 10.4 (Tree Star, Palo Alto, California).
Vera Aguilera J., Paludo J., McWilliams R.R., Zhang H., Li Y., Kumar A.B., Failing J., Kottschade L.A., Block M.S., Markovic S.N., Dong H., Dronca R.S, & Yan Y. (2020). Chemo-immunotherapy combination after PD-1 inhibitor failure improves clinical outcomes in metastatic melanoma patients. Melanoma Research, 30(4), 364-375.
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2

Immunophenotyping of PBMC Populations

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The following panel of antibodies was used for analysis of PBMC populations: CD8-PE-Cy7 (BD Pharmingen, clone RPA-T8, catalog 304006), CD11a-APC (BioLegend, clone HI111, catalog 301212), PD-1 FITC (BioLegend, clone EH12.2H7, catalog 32990), CX3CR1-APC/Cy7 (BioLegend, clone 2A9–1, catalog 341616), Bim-PE (Cell Signaling Technology, clone C34C5, catalog 12186S), Ki-67-BV421(BD Biosciences, clone B56, catalog 562899), Granzyme B-PerCP (Novus Biologicals, clone CLB-GB11, catalog NBP1–50071PCP). CD8+ T-cells were first stained for surface markers followed by intracellular staining. Flow cytometry data was collected on a CytoFLEX LX (Beckman Coulter). Flow cytometry analysis was performed with FlowJo software 10.4 (Tree Star).
Vera Aguilera J., Paludo J., McWilliams R.R., Zhang H., Li Y., Kumar A.B., Failing J., Kottschade L.A., Block M.S., Markovic S.N., Dong H., Dronca R.S, & Yan Y. (2020). Chemo-immunotherapy combination after PD-1 inhibitor failure improves clinical outcomes in metastatic melanoma patients. Melanoma Research, 30(4), 364-375.
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3

Multiparametric Immune Cell Profiling

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Peripheral Blood Mononuclear Cells values were isolated, and subpopulations were identified with the following antibody panel: CD8-PE-Cy7 (BD Pharmingen, clone RPA-T8, catalog 304006), CD11a-APC (BioLegend, clone HI111, catalog 301212), PD-1 FITC (BioLegend, clone EH12.2H7, catalog 32990), Bim-PE (Cell Signaling Technology, clone CD34C5, catalog 12186S), Granzyme B-PerCP (Novus Biologicals, clone CLB0GB11, catalog NBP1–50071PCP), and CX3CR1-APC/Cy7 (BioLegend, clone 2A9–1, catalog 341616). Flow cytometry was performed on a CytoFLEX LX (Beckman Coulter, Atlanta, Georgia). Data were analyzed with FlowJo, version 10.6.1.
Lucien F., Kim Y., Qian J., Orme J.J., Zhang H., Arafa A., Abraha F., Thapa I., Tryggestad E.J., Harmsen W.S., Kosti J., Ali H., Lowe V.J., Johnson G.B., Kwon E.D., Dong H, & Park S.S. (2022). Tumor-Derived Extracellular Vesicles Predict Clinical Outcomes in Oligometastatic Prostate Cancer and Suppress Antitumor Immunity. International journal of radiation oncology, biology, physics, 114(4), 725-737.
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4

Multiparametric Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peripheral Blood Mononuclear Cells values were isolated, and subpopulations were identified with the following antibody panel: CD8-PE-Cy7 (BD Pharmingen, clone RPA-T8, catalog 304006), CD11a-APC (BioLegend, clone HI111, catalog 301212), PD-1 FITC (BioLegend, clone EH12.2H7, catalog 32990), Bim-PE (Cell Signaling Technology, clone CD34C5, catalog 12186S), Granzyme B-PerCP (Novus Biologicals, clone CLB0GB11, catalog NBP1–50071PCP), and CX3CR1-APC/Cy7 (BioLegend, clone 2A9–1, catalog 341616). Flow cytometry was performed on a CytoFLEX LX (Beckman Coulter, Atlanta, Georgia). Data were analyzed with FlowJo, version 10.6.1.
Lucien F., Kim Y., Qian J., Orme J.J., Zhang H., Arafa A., Abraha F., Thapa I., Tryggestad E.J., Harmsen W.S., Kosti J., Ali H., Lowe V.J., Johnson G.B., Kwon E.D., Dong H, & Park S.S. (2022). Tumor-Derived Extracellular Vesicles Predict Clinical Outcomes in Oligometastatic Prostate Cancer and Suppress Antitumor Immunity. International journal of radiation oncology, biology, physics, 114(4), 725-737.
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5

Phenotyping of Cytotoxic T Cells

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PBMC were stained for flow cytometry assays in FACS buffer (1X PBS, 2 mM EDTA and 3% FBS) with the following antibody panel: CD8-PE-Cy7 (BD Pharmingen, clone RPA-T8, catalog 304006), CD11a-APC (BioLegend, clone HI111, catalog 301212), CX3CR1-APC/Cy7 (BioLegend, clone 2A9-1, catalog 341616), Granzyme B-PerCP (Novus Biologicals, clone CLB-GB11, catalog NBP1-50071PCP). Live/Dead fixable stain kit was used to distinguish live cells (Thermo Fisher Scientific, L34961). Data acquisition was performed on a CytoFLEX LX (Beckman Coulter). We analyzed flow cytometry data with FlowJo 10.4 (Tree Star).
An Z., Hsu M.A., Gicobi J.K., Xu T., Harrington S.M., Zhang H., Pavelko K.D., Hirdler J.B., Lohse C.M., Nabavizadeh R., Pessoa R.R., Sharma V., Thompson R.H., Leibovich B.C., Dong H, & Lucien F. (2023). A Novel PD-L1 Antibody Promotes Antitumor Function of Peripheral Cytotoxic Lymphocytes after Radical Nephrectomy in Patients with Renal Cell Carcinoma. Journal of immunology (Baltimore, Md. : 1950), 210(12).
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