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Human immune monitoring 65 plex procartaplex immunoassay kit

Manufactured by Thermo Fisher Scientific
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Sourced in United Kingdom

The Human Immune Monitoring 65-plex ProcartaPlex immunoassay kit is a multiplex assay designed to simultaneously measure the levels of 65 different human immune-related analytes from a single sample. The kit utilizes bead-based technology to provide a comprehensive assessment of the immune system.

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Lab products found in correlation

Bio plex platform, by Bio-Rad (4 mentions) Bio plex pro human cytokine 27 plex immunoassay, by Bio-Rad (2 mentions) Milliplex kit, by Merck Group (2 mentions)

Close spelling variants of this product

ProcartaPlex Human Immune Monitoring 65-plex ProcartaPlex immunoassay kit ProcartaPlex Immunoassay ProcartaPlex kit

4 protocols using human immune monitoring 65 plex procartaplex immunoassay kit

1

Multiplex Immune Protein Profiling

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The preconfigured multiplex Human Immune Monitoring 65-plex ProcartaPlex immunoassay kit (Invitrogen, Thermo Fisher Scientific, UK) was used to measure 65 protein targets in plasma on the Bio-Plex platform (Bio-Rad Laboratories, Hercules, CA, USA), using Luminex xMAP technology. Analytes measured included APRIL; BAFF; BLC; CD30; CD40L; ENA-78; Eotaxin; Eotaxin-2; Eotaxin-3; FGF-2; Fractalkine; G-CSF; GM-CSF; Gro-Alpha; HGF; IFN-Alpha; IFN-gamma; IL-10; IL-12p70; IL-13; IL-15; IL-16; IL-17A; IL-18; IL-1Alpha; IL-1Beta; IL-2; IL-20; IL-21; IL-22; IL-23; IL-27; IL-2R; IL-3; IL-31; IL-4; IL-5; IL-6; IL-7; IL-8; IL-9; IP-10; I-TAC; LIF; MCP-1; MCP-2; MCP-3; M-CSF; MDC; MIF; MIG; MIP-1Alpha; MIP-1Beta; MIP-3Alpha; MMP-1; NGF-Beta; SCF; SDF-1Alpha; TNF-Beta; TNF-Alpha; TNF-R2; TRAIL; TSLP; TWEAK; VEGF-A. All assays were conducted as per the manufacturer’s recommendation.
Fendler A., Au L., Shepherd S.T., Byrne F., Cerrone M., Boos L.A., Rzeniewicz K., Gordon W., Shum B., Gerard C.L., Ward B., Xie W., Schmitt A.M., Joharatnam-Hogan N., Cornish G.H., Pule M., Mekkaoui L., Ng K.W., Carlyle E., Edmonds K., Del Rosario L., Sarker S., Lingard K., Mangwende M., Holt L., Ahmod H., Stone R., Gomes C., Flynn H.R., Agua-Doce A., Hobson P., Caidan S., Howell M., Wu M., Goldstone R., Crawford M., Cubitt L., Patel H., Gavrielides M., Nye E., Snijders A.P., MacRae J.I., Nicod J., Gronthoud F., Shea R.L., Messiou C., Cunningham D., Chau I., Starling N., Turner N., Welsh L., van As N., Jones R.L., Droney J., Banerjee S., Tatham K.C., Jhanji S., O’Brien M., Curtis O., Harrington K., Bhide S., Bazin J., Robinson A., Stephenson C., Slattery T., Khan Y., Tippu Z., Leslie I., Gennatas S., Okines A., Reid A., Young K., Furness A.J., Pickering L., Gandhi S., Gamblin S., Swanton C., Nicholson E., Kumar S., Yousaf N., Wilkinson K.A., Swerdlow A., Harvey R., Kassiotis G., Larkin J., Wilkinson R.J, & Turajlic S. (2021). Functional antibody and T-cell immunity following SARS-CoV-2 infection, including by variants of concern, in patients with cancer: the CAPTURE study. Research Square.
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2

Comprehensive Human Immune Profiling

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The pre-configured multiplex Human Immune Monitoring 65-plex ProcartaPlex immunoassay kit (Invitrogen, Thermo Fisher Scientific) was used to measure 65 protein targets in plasma on the Bio-Plex platform (Bio-Rad Laboratories), using Luminex xMAP technology. Analytes measured included APRIL; BAFF; BLC; CD30; CD40L; ENA-78; eotaxin; eotaxin-2; eotaxin-3; FGF-2; fractalkine; G-CSF; GM-CSF; GRO-alpha; HGF; IFN-α; IFN-γ; IL-10; IL-12p70; IL-13; IL-15; IL-16; IL-17A; IL-18; IL-1α; IL-1β; IL-2; IL-20; IL-21; IL-22; IL-23; IL-27; IL-2R; IL-3; IL-31; IL-4; IL-5; IL-6; IL-7; IL-8; IL-9; IP-10; I-TAC; LIF; MCP-1; MCP-2; MCP-3; M-CSF; MDC; MIF; MIG; MIP-1α; MIP-1β; MIP-3α; MMP-1; NGF-β; SCF; SDF-1α; TNF-β; TNF-α; TNF-R2; TRAIL; TSLP; TWEAK; and VEGF-A. All assays were conducted as per manufacturer recommendations.
Au L., Fendler A., Shepherd S.T., Rzeniewicz K., Cerrone M., Byrne F., Carlyle E., Edmonds K., Del Rosario L., Shon J., Haynes W.A., Ward B., Shum B., Gordon W., Gerard C.L., Xie W., Joharatnam-Hogan N., Young K., Pickering L., Furness A.J., Larkin J., Harvey R., Kassiotis G., Gandhi S., Swanton C., Fribbens C., Wilkinson K.A., Wilkinson R.J., Lau D.K., Banerjee S., Starling N., Chau I, & Turajlic S. (2021). Cytokine release syndrome in a patient with colorectal cancer after vaccination with BNT162b2. Nature Medicine, 27(8), 1362-1366.
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3

Multiplex Human Immune Monitoring Assay

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The preconfigured multiplex Human Immune Monitoring 65-plex ProcartaPlex immunoassay kit (Thermo Fisher Scientific, UK) was used to measure 65 protein targets in plasma. Bio-Plex Pro Human Cytokine 27-Plex Immunoassay (Bio-Rad Laboratories, Hercules, CA, USA) and customized MilliplexTM kits (Millipore, St Charles, MO, USA) were used to measure 30 analytes in the QFT plasma samples from unstimulated (Nil) and TB specific antigen stimulated (Ag) samples. All assays were conducted on the Bio-Plex platform (Bio-Rad Laboratories, Hercules, CA, USA), using Luminex xMAP technology. Additional confirmatory experiments were run on the Meso Scale Discovery Inc (Rockville, MD) platform, using the plasma samples only (not QFT). All assays were conducted as per the manufacturer's recommendation and all analytes measured are listed in Supplementary Table 1.
Wilkinson K.A., Schneider-Luftman D., Lai R., Barrington C., Jhilmeet N., Lowe D.M., Kelly G, & Wilkinson R.J. (2021). Antiretroviral Treatment-Induced Decrease in Immune Activation Contributes to Reduced Susceptibility to Tuberculosis in HIV-1/Mtb Co-infected Persons. Frontiers in Immunology, 12, 645446.
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4

Multiplex Immune Profiling of Plasma

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The preconfigured multiplex Human Immune Monitoring 65-plex ProcartaPlex immunoassay kit (Thermo Fisher Scientific, UK) was used to measure 65 protein targets in plasma. Bio-Plex Pro Human Cytokine 27-Plex Immunoassay (Bio-Rad Laboratories, Hercules, CA, USA) and customized MilliplexTM kits (Millipore, St Charles, MO, USA) were used to measure 30 analytes in the QFT plasma samples from unstimulated (Nil) and TB specific antigen stimulated (Ag) samples. All assays were conducted on the Bio-Plex platform (Bio-Rad Laboratories, Hercules, CA, USA), using Luminex xMAP technology. Additional confirmatory experiments were run on the Meso Scale Discovery Inc (Rockville, MD) platform, using the plasma samples only (not QFT). All assays were conducted as per the manufacturer's recommendation and all analytes measured are listed in Supplementary Table 1.
Wilkinson K.A., Schneider-Luftman D., Lai R., Barrington C., Jhilmeet N., Lowe D.M., Kelly G., & Wilkinson R.J. (2020). Antiretroviral treatment-induced decrease in immune activation contributes to reduced susceptibility to tuberculosis in HIV-1-TB co-infected persons.
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