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Clone 28e1

Manufactured by Cell Signaling Technology
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Clone 28E1 is a Mouse monoclonal antibody that recognizes total PARP. It is suitable for use in Western Blotting, Immunohistochemistry, and Immunofluorescence applications.

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Lab products found in correlation

Clone ga5, by Cell Signaling Technology (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Lsm 880, by Zeiss (2 mentions) Alexa fluor 555 phalloidin, by Thermo Fisher Scientific (1 mentions) Donkey anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions) Alexa fluor 647, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

PDGFRB (clone 28E1, (2 citations)

4 protocols using clone 28e1

1

Histopathological Analysis of Zika Virus Infection in Fetal Brain

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Fetal human brain and mouse brain tissues were fixed in 4% paraformaldehyde (PFA) and embedded in paraffin. Ten-micron sections were dewaxed, rehydrated and stained with hematoxylin and eosin (H&E), Alizarin Red S, Von Kossa stain or RNAscope. RNAscope in situ hybridization was performed with specific ZIKV-H/PF/2013 polyprotein probes (RNAscope Probe-V-ZIKA-pp; ACDbio, 463781) and human PDGFRβ probes (RNAscope Probe-Hs-PDGFRB-C2; ACDbio, 548991-C2) according to manufacturer’s protocol. Followed by immunohistochemical staining with anti-human PDGFRβ (1:100 dilution; clone 28E1, Cell Signaling Technology, #3169S), GFAP (1:50 dilution; clone GA5, Cell Signaling Technology, #3670S), NeuN (1:50 dilution; Millipore, #ABN78) antibodies and counterstained with hematoxylin.
Chen W., Foo S.S., Hong E., Wu C., Lee W.S., Lee S.A., Evseenko D., Lopes Moreira M.E., García-Sastre A., Cheng G., Nielsen-Saines K., Brasil P., Avvad-Portari E, & Jung J.U. (2021). Zika virus NS3 protease induces bone morphogenetic protein-dependent brain calcification in human fetuses. Nature microbiology, 6(4), 455-466.
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2

Endosialin and PDGFR-β Expression in Tumor Biopsies

Cited 1 time
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Tissue endosialin expression was assessed on tumor biopsy samples (paired sampling) obtained either at screening or from archival blocks and between Days 22 and 28 of Cycle 1 in a subset of 30 patients. PDGFR-β tumor tissue expression was assessed at screening only.
Formalin-fixed-paraffin-embedded slides were prepared and assessed for endosialin and PDGFR-β expression by immunohistochemistry using the previously described rat anti-human endosialin 9G5 monoclonal antibody [30 (link)] or clone 28E1 (Cell Signaling, Danvers, MA, USA) for PDGFR-β [20 (link), 26 ] by Quintiles Laboratories Americas (Marietta, GA and Westmont, IL). Expression was reported as +1, +2, or +3 as assessed by a single board certified pathologist.
Baseline serum samples were collected for evaluation of soluble endosialin using electrochemiluminescent assays performed by Frontage Laboratories, Inc. (Exton, PA) using endosialin-specific monoclonal antibodies [30 (link)].
D’Angelo S.P., Hamid O.A., Tarhini A., Schadendorf D., Chmielowski B., Collichio F.A., Pavlick A.C., Lewis K.D., Weil S.C., Heyburn J., Schweizer C., O’Shannessy D.J, & Carvajal R.D. (2017). A PHASE 2 STUDY OF ONTUXIZUMAB, A MONOCLONAL ANTIBODY TARGETING ENDOSIALIN, IN METASTATIC MELANOMA. Investigational new drugs, 36(1), 103-113.
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3

3D Confocal Imaging of Breast Tissue

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For 3D confocal imaging, breast tissue was prepared and imaged as previously described (Rios et al,2019). Briefly, fresh tissue from reduction mammoplasty surgeries (pathologically normal) was fixed in 4% paraformaldehyde in PBS for 2 h, and then, samples were washed prior to immunolabeling overnight with the following primary antibodies: rat monoclonal E‐cadherin (Thermo Fisher Scientific, Clone ECCD‐2, Cat#13‐1900; 1:250 dilution) and rabbit monoclonal platelet‐derived growth factor receptor ß (Cell Signaling, Clone 28E1, Cat# 3169; 1:100 dilution). After washing steps, samples were then incubated overnight with fluorescently conjugated secondary antibodies: donkey anti‐rat (H + L) Alexa Fluor 488 (Thermo Fisher Scientific, Cat# A‐21208) and donkey anti‐rabbit IgG (H + L) (Thermo Fisher Scientific, Cat# A‐31573) Alexa Fluor 647 together with Phalloidin Alexa Fluor 555 (Thermo Fisher Scientific, Cat# A‐34055) and DAPI (Thermo Fisher Scientific, Cat# 62248). Immunolabeled samples were subsequently cleared using FUnGI prior to dissection and mounting. Confocal imaging was performed using a Zeiss LSM 880 or 980 inverted microscope using a 40×, 1.3 N.A. oil objective. Image processing and visualization was performed in Zen (Zeiss) and Imaris (Bitplane) software.
Pal B., Chen Y., Vaillant F., Capaldo B.D., Joyce R., Song X., Bryant V.L., Penington J.S., Di Stefano L., Tubau Ribera N., Wilcox S., Mann G.B., Papenfuss A.T., Lindeman G.J., Smyth G.K, & Visvader J.E. (2021). A single‐cell RNA expression atlas of normal, preneoplastic and tumorigenic states in the human breast. The EMBO Journal, 40(11), e107333.
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4

Histopathological Analysis of Zika Virus Infection in Fetal Brain

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fetal human brain and mouse brain tissues were fixed in 4% paraformaldehyde (PFA) and embedded in paraffin. Ten-micron sections were dewaxed, rehydrated and stained with hematoxylin and eosin (H&E), Alizarin Red S, Von Kossa stain or RNAscope. RNAscope in situ hybridization was performed with specific ZIKV-H/PF/2013 polyprotein probes (RNAscope Probe-V-ZIKA-pp; ACDbio, 463781) and human PDGFRβ probes (RNAscope Probe-Hs-PDGFRB-C2; ACDbio, 548991-C2) according to manufacturer’s protocol. Followed by immunohistochemical staining with anti-human PDGFRβ (1:100 dilution; clone 28E1, Cell Signaling Technology, #3169S), GFAP (1:50 dilution; clone GA5, Cell Signaling Technology, #3670S), NeuN (1:50 dilution; Millipore, #ABN78) antibodies and counterstained with hematoxylin.
Chen W., Foo S.S., Hong E., Wu C., Lee W.S., Lee S.A., Evseenko D., Lopes Moreira M.E., García-Sastre A., Cheng G., Nielsen-Saines K., Brasil P., Avvad-Portari E, & Jung J.U. (2021). Zika virus NS3 protease induces bone morphogenetic protein-dependent brain calcification in human fetuses. Nature microbiology, 6(4), 455-466.
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