The IS6110-LAMP and IS1081-LAMP reaction mixtures were performed at the temperature ranging from 61°C to 68°C at 1°C intervals for 1 h to determine the optimal reaction temperature. The amplifications were monitored by Loopamp LA-320C Realtime Turbidimeter (Eiken, Janpan). Genomic DNA of Streptococcus pneumoniae (1 ng) and Escherichia coli (1 ng) was used as negative control. The IS6110-LAMP and IS1081-LAMP reactions were performed three times each.
Loopamp la 320c realtime turbidimeter
Manufactured by Eiken Chemical
Sourced in Japan
The Loopamp LA-320C Realtime Turbidimeter is a laboratory instrument designed to measure the turbidity of liquid samples. It utilizes real-time detection technology to provide accurate and reliable measurements of the cloudiness or haze present in the sample. The core function of this device is to quantify the level of suspended particles or solids in a liquid, which is a fundamental parameter in various analytical and quality control applications.
Automatically generated - may contain errors
Lab products found in correlation
Nanodrop nd 1000 spectrophotometer, by Thermo Fisher Scientific (3 mentions)
Agarose, by Avantor (1 mentions)
Magnesium sulfate, by Merck Group (1 mentions)
Mycoplasma supplement g, by Thermo Fisher Scientific (1 mentions)
Mycoplasma broth base, by Thermo Fisher Scientific (1 mentions)
Genomic dna purification kit, by Promega (1 mentions)
2 protocols using loopamp la 320c realtime turbidimeter
1
Optimizing LAMP Assays for Pneumococcus and E. coli
2
Rapid Mycoplasma Detection by LAMP
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mycoplasma broth base (CM0403, Oxoid, United Kingdom); Mycoplasma Supplement-G (SROO59C, Oxoid); Bst (Bacillus stearothermophilus) DNA polymerase, large fragment (New England Biolabs, Boston); Genomic DNA Purification Kit (Promega); real-time PCR kit (Da An Gene Co., Sun Yat-Sen University, China); dNTPs (Pharmacia); Chelex-100 and betaine (Sigma); magnesium sulfate, potassium chloride, sodium hydroxide, EDTA, and ammonium sulfate (Sinopharm Chemical Reagents, China); Tris-HCl (Shanghai Simco Biotech, Shanghai, China); Triton X-100 (Beijing Mylab Corporation, China); NP-40 (FLUKA); hydroxynaphthol blue (HNB; Regal Biotechnology, China); 2× Taq Mix (Tiangen Biotech, China); agarose (AMRESCO); thermal cycler, gel imager, and CFX96 real-time system (Bio-Rad, California); NanoDrop ND-1000 spectrophotometer (Thermo Scientific); Loopamp LA-320c Realtime Turbidimeter (Eiken Chemical Co., Tokyo, Japan); block heater (Hangzhou Bioer Technology, Hangzhou, China). Primers were synthesized by Beijing Sangon Biotech.
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