Innova 4000 shaker

Manufactured by Eppendorf

The Innova 4000 is a benchtop shaker designed for use in laboratory settings. It provides reliable and consistent mixing of samples in a temperature-controlled environment. The shaker features adjustable speed and orbit settings to accommodate a variety of applications.

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Lab products found in correlation

Bovine lactoferrin, by Merck Group (1 mentions) Rnase b, by Merck Group (1 mentions) L cysteine, by Merck Group (1 mentions)

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Innova 4000 (21 citations) Innova 4000 Benchtop Incubator Shaker (7 citations)

3 protocols using innova 4000 shaker

Culturing Bacterial Strains in LB and CAA

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Bacterial strains and plasmids used in this study are described in Table 1. Bacteria were grown at 37°C in Lysogenic broth (LB) medium (Life Technologies) or in iron‐poor Casamino Acids (CAA) medium (Difco Laboratories). Liquid cultures were shaken in a New Brunswick Innova 4000 shaker at 200 rpm.

Dingemans J., Ghequire M.G., Craggs M., De Mot R, & Cornelis P. (2016). Identification and functional analysis of a bacteriocin, pyocin S6, with ribonuclease activity from a Pseudomonas aeruginosa cystic fibrosis clinical isolate. MicrobiologyOpen, 5(3), 413-423.

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Cloning and Purification of Bovine Proteins

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The B. longum subsp. Infantis ATCC 15697 strain used in this study was obtained from the University of California Davis Viticulture and Enology Culture Collection (Davis, CA). Bifidobacterium was grown in Man–Rogose–Sharp (MRS) broth supplemented with 0.05% (w/v) l-cysteine (Sigma-Aldrich). The cells were anaerobically grown in a vinyl chamber (Coy Laboratory Products, Grass Lake, MI) at 37°C for 24 h, in an atmosphere consisting of 5% carbon dioxide, 5% hydrogen, and 90% nitrogen. The E. coli Bl21* strain used for protein expression was grown in Luria Broth (LB) media containing Carbenicillin (100 μg/mL). All incubations were carried out in an Innova 4000 shaker (New Brunswick Scientific, New Jersey) at 200 rpm and 37°C. Bovine lactoferrin and RNase B were obtained from Sigma-Aldrich (St. Louis, MO, USA). Gene cloning, expression, and purification and pilot-scale production of protein concentrate from bovine colostrum whey were performed as described before.²⁷

Karav S., Bell J.M., Le Parc A., Liu Y., Mills D.A., Block D.E, & Barile D. (2015). Characterizing the Release of Bioactive N-Glycans from Dairy Products by a Novel Endo-β-N-Acetylglucosaminidase. Biotechnology progress, 31(5), 1331-1339.

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Yeast and E. coli Culture Protocols

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All S. cerevisiae strains used in this study are derived from the CEN.PK family (20 ). For non-selective growth and propagation, the yeast strains were grown on Yeast extract-Peptone (YP) medium containing: 10 g l⁻¹ Bacto yeast extract and 20 g l⁻¹ Bacto peptone. For selective growth, Synthetic Medium (SM) was used (21 (link)). 20 g l⁻¹ glucose was used as carbon source. Liquid yeast culture were grown in 500 ml shake flask with 100 ml medium at 30°C and 200 rpm in an Innova incubator (New Brunswick Scientific, Edison, NJ, USA), unless stated otherwise.
Escherichia coli XL1-blue was used for propagation and isolation of plasmids. E. coli was grown in Lysogeny Broth (10 g l⁻¹ Bacto tryptone, 5 g l⁻¹ Bacto yeast extract and 5 g l⁻¹ NaCl). Cultivation was performed in 15 ml Greiner tubes or 25 ml shake flasks at 37°C and 200 rpm in an Innova 4000 shaker (New Brunswick Scientific).
For storage of S. cerevisiae and E. coli strains, the cultures were mixed with glycerol (30% v/v) and stored in 1 ml vials at −80°C.

Postma E.D., Dashko S., van Breemen L., Taylor Parkins S.K., van den Broek M., Daran J.M, & Daran-Lapujade P. (2021). A supernumerary designer chromosome for modular in vivo pathway assembly in Saccharomyces cerevisiae. Nucleic Acids Research, 49(3), 1769-1783.

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