Colo 320dm

Manufactured by Thermo Fisher Scientific

Sourced in United States

The COLO 320DM is a cell line derived from a human colon adenocarcinoma. It is commonly used in cell-based assays and research applications.

Automatically generated - may contain errors

Lab products found in correlation

5 protocols using colo 320dm

Colorectal Cancer Cell Lines and Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human CRC cell lines LS180, RKO, SW48, HCT15, HCT116 and Colo320DM were obtained from ATCC (LGC Standards, Wesel, Germany). LS180, RKO and SW48 were kept in MEM (Invitrogen) supplemented with 15% FBS, 1% essential amino acids and antibiotics. HCT15, HCT116 and Colo320DM were kept in RPMI (Invitrogen) supplemented with 10% FBS plus antibiotics. Adult dermal fibroblasts (HDFa, PCS-201-012) and neonatal fibroblasts (HDFn, PCS-201-010) were obtained from ATCC. Fibroblasts were cultured with fibroblast basal medium (FBM, PCS-201-030 from ATCC) supplemented with low serum fibroblast growth kit (ATCC, PCS-201-041). Cells were cultivated at 37 °C in 5% CO₂. Irradiation was done using the RS320 X-Ray machine by XStrahl Ltd. at 300 kV, 10 mA, dose rate 0,9 Gy/min.

Niedermaier B., Sak A., Zernickel E., Xu S., Groneberg M, & Stuschke M. (2019). Targeting ARID1A-mutant colorectal cancer: depletion of ARID1B increases radiosensitivity and modulates DNA damage response. Scientific Reports, 9, 18207.

+ Open protocol

+ Expand

Kras Mutant Colon Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Six human colon cancer cell lines with documented Kras G12D mutations (Kras^G12D), LS-174T, SNU-407, and SNU-C2A, and three Kras wild-type (Kras^WT) colon cancer cells, Caco2, COLO-320DM, and HT29, were purchased from American Type Culture Collection (ATCC, Manassas, VA. USA) or Korea Cell Line Bank (KCLB, Seoul, Korea). Enriched cells were then cultured at sub-clonal density (1–10 cells/cm²) with complete growth medium of each cell line at 37 °C and 5% CO₂ atmosphere. Caco2 was cultured in MEM medium (Gibco-Invitrogen, Waltham, MA, USA) supplemented with 20% FBS (Gibco-Invitrogen) at 37 °C and 5% CO₂; LS-174T, SNU-407, SNU-C2A, HT-29, and COLO-320DM were cultured in RPMI 1640 medium (Gibco-Invitrogen) with 10% FBS. For the palmitate treatments, cells were treated with 50 μM conjugated palmitate (Sigma-Aldrich) with 10% (w/v) fatty acid free BSA (Sigma-Aldrich) in the DMEM medium. Cell lines used in silico analysis, in vitro, and in vivo experiments, as listed in Table S2.

Song J., Park S., Oh J., Kim D., Ryu J.H., Park W.C., Baek I.J., Cheng X., Lu X, & Jin E.J. (2020). NUDT7 Loss Promotes KrasG12D CRC Development. Cancers, 12(3), 576.

+ Open protocol

+ Expand

Colorectal Cell Line Maintenance and Transfection

Check if the same lab product or an alternative is used in the 5 most similar protocols

CCD-841 CoN (CRL-1790, RRID:CVCL_2871), HCT116 (CCL-247, RRID:CVCL_0291), SW480 (CCL-228, RRID:CVCL_0546), COLO 320DM (CCL-220, RRID:CVCL_0219), Caco-2 (HTB-37, RRID:CVCL_0025), and HUVEC (CRL-1730, RRID:CVCL_2959) were purchased from American Type Culture Collection (Manassas) and maintained at 37°C and 5% CO₂. CCD-841 CoN were cultured in Eagle’s minimum essential medium (Gibco, 61100061) supplemented with 10% FBS, HCT116 was cultured in McCoy’s 5A (Sigma-Aldrich, M4892) supplemented with 10% FBS, SW480 and COLO 320DM were cultured in RPMI 1640 (Gibco, 31800022) supplemented with 10% FBS, Caco-2 was cultured in Eagle’s minimum essential medium supplemented with 20% FBS, and HUVEC were cultured in medium 199 (Gibco, 31100-035) supplemented with heparin (25 U/ml; Sigma-Aldrich, H3149), endothelial cell growth supplement (30 μg/ml; Millipore, 02-102), and 10% FBS. Transient transfections were done using Lipofectamine 2000 (Invitrogen, 11668019), following the manufacturer’s protocol, and cultured for 1 day with or without ARP100 or RVX-208 treatment before samples were processed. For EMT marker analysis, cells were collected after 5 days of the transfection with or without ARP100 or RVX-208 treatment. For the generation of stable clones, transfected HCT116 cells were cultured for several weeks in media containing hygromycin B (100 μg/ml; Roche, 31282-04-9) selection.

Hsu P.L., Chien C.W., Tang Y.A., Lin B.W., Lin S.C., Lin Y.S., Chen S.Y., Sun H.S, & Tsai S.J. (2023). Targeting BRD3 eradicates nuclear TYRO3-induced colorectal cancer metastasis. Science Advances, 9(15), eade3422.

+ Open protocol

+ Expand

Authenticated Colorectal Cancer Cell Lines Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Authenticated CRC lines were obtained from ECACC: HT29 (ECACC 91072201), Colo205 (ECACC 87061208), Colo320DM (ECACC 87061205), SW480 (ECACC 87092801), and SW620 (ECACC 87051203). Authenticated primary human colonocytes were obtained from ATCC: CCD-18Co (ATCC CRL-1459) and CCD-841 CoN (ATCC CRL-1790). All lines were regularly monitored for mycoplasma contamination every 2 months by PCR^{40 (link)}, and only mycoplasma-free cells were used for studies. All cells were used for no more than 15 passages. All cells were grown at 37 °C in HEPA-filtered humidified air in 5% CO₂, in media supplemented with 10% heat-inactivated FBS (Gibco, Paisley, Scotland, UK, Cat. no. 10099-141), L-Glu (2 mM, Lonza, Burton on Trent, UK, Cat. no. 17-606E) and penicillin/streptomycin (100 U/ml, Gibco, Cat. no. DE17-602E). Colo205 and Colo320DM cells were grown in RPMI medium (Gibco, Paisley, Scotland, UK, Cat. no. 31870), HT29 were grown in McCoy’s medium (Lonza, Burton on Trent, UK, Cat. no. 12-688F), SW480 and SW620 were grown in L15 medium (Lonza, Burton on Trent, UK, Cat. no. BE12-700F), and HCT-116 (17) were grown in DMEM (Gibco, Paisley, Scotland, UK, Cat. no. 21885-025). CCD-18Co and CCD-841 primary cultures were grown in EMEM (Sigma-Aldrich, Saint Louis, MO, USA, Cat. no. M2279).

Stramucci L., Pranteda A., Stravato A., Amoreo C.A., Pennetti A., Diodoro M.G., Bartolazzi A., Milella M, & Bossi G. (2019). MKK3 sustains cell proliferation and survival through p38DELTA MAPK activation in colorectal cancer. Cell Death & Disease, 10(11), 842.

+ Open protocol

+ Expand

Cell Culture Conditions for Various Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

NIH3T3 mouse embryonic fibroblasts were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The human colorectal cancer cell lines SW48 and COLO320DM were purchased from Cobioer Biosciences (Nanjing, China). HEK293T cells and HEK293F cells were kindly provided by Professor Dr. Linqi Zhang (Tsinghua University, China). NIH3T3 cells, HEK293T cells, and SW48 cells were cultured in DMEM (Gibco, Grand Island, NY, USA) supplemented with 10% (v/v) fetal bovine serum (Gibco, MA, USA) and 1% (v/v) penicillin/streptomycin (Solarbio, Beijing, China) at 37 °C in a humidified atmosphere containing 5% CO₂. COLO320DM cells were cultured in RPMI-1640 medium (Gibco, Grand Island, NY, USA) supplemented with 10% (v/v) fetal bovine serum (Gibco, MA, USA) and 1% (v/v) penicillin/streptomycin (Solarbio, Beijing, China) at 37 °C in a humidified atmosphere containing 5% CO₂. HEK293F cells were cultured in SMM 293-TI medium (SinoBiological, China) supplemented with 0.5% (v/v) fetal bovine serum and 1% (v/v) penicillin/streptomycin at 37 °C under 5% CO₂ in a New Brunswick S41i shaking incubator (Eppendorf, 120 rpm).

Zhuang X., Wang Z., Fan J., Bai X., Xu Y., Chou J.J., Hou T., Chen S, & Pan L. (2022). Structure-guided and phage-assisted evolution of a therapeutic anti-EGFR antibody to reverse acquired resistance. Nature Communications, 13, 4431.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!