The CCK8 assays were performed as instructed by the manufacturer to assess cell viability. Briefly, the cells were seeded in 96-well plates (approximately 3000 cells/well). GNPs and GAL-PEG-GNPs were diluted to various concentrations in 1× PBS (pH 7.4), then added into the wells. After 24 h incubation, 20 μL CCK8 (keygentec company, Nanjing, Jiangsu, China) was added to each well for 4 h. Optical density (OD) was measured at 490 nm using a Microplate reader (BioRad, DG3022, USA) and the proliferation index was calculated as experimental OD value/control OD value. Estimate value of 50 % inhibition concentration (IC50).
Dg3022
Manufactured by Bio-Rad
Sourced in United States
The DG3022 is a digital gradient maker produced by Bio-Rad. It is designed to create linear gradients for use in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dg3022
1
Cell Viability Assay with GNPs
2
Cytotoxicity Evaluation of C225, GNPs, and C225-GNPs
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Cell Counting Kit-8 (CCK8; Nanjing KeyGen Biotech Co., Ltd.) assays were used to determine the cytotoxicity of C225, GNPs and C225-GNPs. The assays were performed according to the manufacturer's protocol to assess cell viability. The cells were cultured at a density of 3×103 cells per well in flat-bottomed 96-well plates. The C225, GNPs and C225-GNPs were diluted to various concentrations in 1X PBS (Ph 7.4), and then added into the wells and incubated for 24 h at 37°C, followed by exposure to 10 µl CCK8, which was added to each well for 2 h. The absorbance was measured at 490 nm using a microplate reader (DG3022; Bio-Rad Laboratories, Inc., Hercules, CA, USA) and the 50% inhibition concentration (IC50) value was estimated.
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