Mvx10 dissecting microscope

All specimens studied were adults. Genitalia extractions and preservation largely followed the methodology described by Smith (1979) . Specimens were examined using Wild M5A and Olympus SZ61 stereoscopes. Measurements were made using an ocular scale in an Olympus WHSZ10X-H/22 eyepiece on an Olympus SZ61 stereoscopic microscope. Microphotography employed an Olympus SzX12 dissecting microscope equipped with an MTI 3CCD camera and an Olympus MVX10 dissecting microscope equipped with an Olympus DP70 camera. Image montage employed Olympus cellSens software. Images were processed with Adobe Photoshop 2020 (21.1.2). Descriptive terminology largely follows Konstantinov (1998) .
Specimens are deposited in the Monte L. Bean Life Science Museum, Brigham Young University (Provo, Utah, U.S.A.; BYUC), Museo de Zoología de la Pontificia Universidad Católica del Ecuador (Quito, Ecuador; QCAZ), and Travancore Insect Collection, Kerala Agricultural University (Vellayani, Kerala, India; TIC). Label data from the specimens are presented verbatim; a backwards slash (\) indicates a new line on a label.

For fluorescence imaging (hsp-6p::gfp), worms were anesthetized with 25 mM levamisole, and photographs were taken using an Olympus MVX10 dissecting microscope with a DP80 camera. To quantify fluorescent intensity, the entire intestine regions were outlined and quantified using ImageJ software. The fluorescence intensity was then normalized to the body area.
For quantifying BCF-1::GFP, the worms were mounted on 2% agarose pads with 25 mM levamisole and imaged using an Olympus MVX10 microscope with a DP80 camera.

Whole-mount in situ hybridization was performed as previously described [48 (link),49 (link)]. Linearized pGEMTeasy templates containing adamts9 or adamts15a cDNA inserts amplified as described above were column purified and 1 μg of template used for in vitro transcription of digoxygenin-labelled sense or antisense RNA probes. Embryos were imaged on an Olympus, MVX10 dissecting microscope.