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Low viscosity sodium alginate

Manufactured by Merck Group
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Low viscosity sodium alginate is a polysaccharide derived from brown seaweed. It has a low viscosity, making it suitable for various industrial and laboratory applications.

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Lab products found in correlation

Brookfield viscosity 4 12 cps, by Merck Group (3 mentions) Cholic acid, by Merck Group (2 mentions) Probucol, by Merck Group (2 mentions) Alloxan, by Merck Group (1 mentions) 20 70 dichlorofluorescin diacetate dcfh da, by Merck Group (1 mentions) High molecular weight chitosan, by Merck Group (1 mentions) Gliclazide, by Merck Group (1 mentions) Calcium chloride dehydrate, by Scharlab (1 mentions) D mannitol, by Merck Group (1 mentions) Glacial acetic acid, by Merck Group (1 mentions) Cacl2, by Merck Group (1 mentions) 4 arm peg acrylate, by Laysan Bio (1 mentions) Dextran sulfate, by Merck Group (1 mentions) Trifluoroacetic acid 99, by Merck Group (1 mentions) Nile red, by Merck Group (1 mentions) Sodium citrate dehydrate, by Merck Group (1 mentions) Actrapid, by Novo Nordisk (1 mentions) Lutrol f68, by BASF (1 mentions) Low molecular weight chitosan, by Thermo Fisher Scientific (1 mentions) Ursodeoxycholic acid, by Merck Group (1 mentions)

14 protocols using low viscosity sodium alginate

1

Antioxidant Activity Evaluation Protocol

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PB (99.89%), sodium alginate low-viscosity (99%), LCA (≥95%), and alloxan (>98%) were obtained from Sigma-Aldrich CO. (St. Louis, MO, USA). Calcium chloride dihydrates (CaCl2·2H2O, 98%) were purchased from Scharlab S.L (Barcelona, Spain). Ultrasonic gel was purchased from the Australian Medical Association (Perth, WA, Australia). 20,70-Dichlorofluorescin diacetate (DCFH-DA) was purchased from Sigma-Aldrich Corporation CO., (St. Louis, MO, USA) and 2,2′-Azobis-2-methyl-propanimidamide, dihydrochloride (AAPH) purchased from Sapphire Bioscience (Redfern, NSW, Australia).
Wagle S.R., Kovacevic B., Ionescu C.M., Walker D., Jones M., Carey L., Takechi R., Mikov M., Mooranian A, & Al-Salami H. (2021). Pharmacological and Biological Study of Microencapsulated Probucol-Secondary Bile Acid in a Diseased Mouse Model. Pharmaceutics, 13(8), 1223.
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2

Alginate-based Hydrogel Synthesis

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PB (99.89%) was purchased from Medisca (Las Vegas, NV, USA), sodium alginate low viscosity (99%) and ULCA (≥95%) were purchased from Sigma-Aldrich CO., (St Louis, MO, USA). Calcium chloride dehydrates (CaCl2.2H2O, 98%) were purchased from Scharlab S.L (Sentmenat, Spain).
Wagle S.R., Kovacevic B., Walker D., Ionescu C.M., Jones M., Stojanovic G., Kojic S., Mooranian A, & Al-Salami H. (2020). Pharmacological and Advanced Cell Respiration Effects, Enhanced by Toxic Human-Bile Nano-Pharmaceuticals of Probucol Cell-Targeting Formulations. Pharmaceutics, 12(8), 708.
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3

Encapsulation of Yeast Cells

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Starting material dry yeast (Saccharomyces cerevisae) was purchased from a nearby authentic bakery (Polo Enterprises, Lucknow). Sodium alginate low viscosity (a viscosity of 2 % solution: 100-300 cP) was purchased from Sigma Aldrich. Rhodamine B (Rd B) was purchased from sigma Aldrich. All other analytical chemicals and solvents used in the study were HPLC grade purchased from Merck (Mumbai, India). All aqueous solutions were prepared from triple distilled water (TDW).
Upadhyay T.K., Fatima N., Sharma D., Saravanakumar V, & Sharma R. (2017). Preparation and characterization of beta-glucan particles containing a payload of nanoembedded rifabutin for enhanced targeted delivery to macrophages. EXCLI Journal, 16, 210-228.
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4

Alginate-Chitosan Hydrogel Formulation

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Low viscosity sodium alginate (CAS Number: 9005-38-3); Brookfield viscosity 4–12 cps (1% in H2O at 25 °C) was purchased from Sigma Aldrich (USA). High molecular weight chitosan (CAS Number: 9012-76-4, molecular weight: 310–375 kDa) was obtained from Sigma Aldrich (St. Louis, USA). A commercially available product calcium chloride, CaCl2 was used as a calcium donating substance (Kemika, Zagreb, Croatia). All other chemicals were of analytical grade and used as received without further purification. Derivatives of dehydroamino acids were synthesized in the laboratory of professor Alexander Prosyanik (Dnipro, Ukraine).
Vlahoviček-Kahlina K., Jurić S., Marijan M., Mutaliyeva B., Khalus S.V., Prosyanik A.V, & Vinceković M. (2021). Synthesis, Characterization, and Encapsulation of Novel Plant Growth Regulators (PGRs) in Biopolymer Matrices. International Journal of Molecular Sciences, 22(4), 1847.
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5

Gliclazide-Alginate Microcapsule Formulation

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Gliclazide (99.92 %), low-viscosity sodium alginate (99 %) and cholic acid (98 %) were purchased from Sigma Chemical Co, USA. Calcium chloride dehydrate (98 %) was obtained from Scharlab S.L, Australia. All solvents and reagents were supplied by Merck (Australia) and were of HPLC grade and used without further purification.
Mooranian A., Negrulj R., Mathavan S., Martinez J., Sciarretta J., Chen-Tan N., Mukkur T., Mikov M., Lalic-Popovic M., Stojančević M., Golocorbin-Kon S, & Al-Salami H. (2014). Stability and Release Kinetics of an Advanced Gliclazide-Cholic Acid Formulation: The Use of Artificial-Cell Microencapsulation in Slow Release Targeted Oral Delivery of Antidiabetics. Journal of Pharmaceutical Innovation, 9(2), 150-157.
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6

Microfluidic Synthesis of Fluorescent Extracellular Vesicles

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The poly(dimethylsiloxane) (PDMS) flow-focusing device and the microchannel were fabricated with SYLGARD™ 184 Silicone Elastomer Base (Krayden, Denver, CO) and SYLGARD™ 184 Silicone Elastomer Curing Agent (Krayden, Denver, CO). The dispersed phase contained low viscosity sodium alginate (Sigma-Aldrich, St. Louis, MO) and 50 nm CaCO3 nanoparticles (US Research Nanomaterials, Inc., Houston, TX) dissolved in D-mannitol (Sigma-Aldrich, St. Louis, MO). The continuous phase contained 3M™ Novec™ 7500 Engineered Fluid (Thermo Fisher Scientific, Waltham, MA) and Pico-Surf™ 1 (Sphere Fluidics Limited, Cambridge, UK). The microdroplets were crosslinked with glacial acetic acid (Sigma-Aldrich, St. Louis, MO), demulsified with 1H,1H,2H,2H-perfluoro-1-octanol (PFO) (Sigma-Aldrich, St. Louis, MO), and were further crosslinked with CaCl2 (Sigma-Aldrich, St. Louis, MO). Fluorescent EVμBRs were generated by coating the microgels with 30 – 50 kDa poly-L-lysine hydrobromide FITC (Sigma-Aldrich, St. Louis, MO). Poly(ethylene glycol) (PEG) traps were fabricated with the PRIMO optical module (Alvéole, Paris, FR), 10 kDa 4-arm-PEG-acrylate (Laysan Bio, Inc., Arab, AL), and PLPP (Alvéole, Paris, FR).
Rima X.Y., Zhang J., Nguyen L.T., Rajasuriyar A., Yoon M.J., Chiang C.L., Walters N., Kwak K.J., Lee L.J, & Reátegui E. (2022). Microfluidic harvesting of breast cancer tumor spheroid-derived extracellular vesicles from immobilized microgels for single-vesicle analysis. Lab on a chip, 22(13), 2502-2518.
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7

Alginate-Based Microparticles for Insulin Encapsulation

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Low-viscosity sodium alginate (viscosity of 1% solution at 25°C, 4–12 cP), dextran sulfate, sorbitan monooleate (Span 80®), phosphotungstic acid, trifluoroacetic acid 99%, and acetonitrile (LiChrosolv) high-performance liquid chromatography (HPLC) grade were all obtained from Sigma-Aldrich Co. (St Louis, MO, USA); calcium carbonate (Setacarb; Omya, Orgon, France), paraffin oil (Scharlau, Barcelona, Spain), insulin 100 IU/mL (Actrapid®; Novo Nordisk A/S, Bagsværd, Denmark), poloxamer 188 (Lutrol®F68; BASF, Ludwigshafen, Germany), sodium citrate dehydrate (Sigma-Aldrich Co., St Louis, MO, USA), and Nile red (Sigma-Aldrich Co.) were also obtained.
Lopes M.A., Abrahim-Vieira B., Oliveira C., Fonte P., Souza A.M., Lira T., Sequeira J.A., Rodrigues C.R., Cabral L.M., Sarmento B., Seiça R., Veiga F, & Ribeiro A.J. (2015). Probing insulin bioactivity in oral nanoparticles produced by ultrasonication-assisted emulsification/internal gelation. International Journal of Nanomedicine, 10, 5865-5880.
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8

Alginate-Chitosan Nanoparticle Synthesis

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Low viscosity sodium alginate (CAS Number: 9005-38-3; Brookfield viscosity 4–12 cps (1% in H2O at 25 °C) was purchased from Sigma Aldrich (USA). Low molecular weight chitosan (CAS RN: 9012-76-4, molecular weight: 100,000–300,000 Da) was obtained from Acros Organic (USA). A commercially available product calcium chloride, CaCl2 was used as a calcium donating substance (Kemika Croatia). All other chemicals were of analytical grade and used as-received without further purification. Trans-diaqua-trans-bis [1-hydroxy-1,2-di (methoxycarbonyl) ethenato] copper was synthesized in the laboratory of Professor Alexander Prosyanik (Ukraine).
Kudasova D., Mutaliyeva B., Vlahoviček-Kahlina K., Jurić S., Marijan M., Khalus S.V., Prosyanik A.V., Šegota S., Španić N, & Vinceković M. (2021). Encapsulation of Synthesized Plant Growth Regulator Based on Copper(II) Complex in Chitosan/Alginate Microcapsules. International Journal of Molecular Sciences, 22(5), 2663.
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9

Preparation of Probucol-UDCA Formulation

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Probucol, metformin, low viscosity sodium alginate, ursodeoxycholic acid and sodium alginate were purchased from Sigma Chemical Co, USA, while calcium chloride from Scharlab S.L, Australia. The reagents were purchased from Merck (Australia) and were used without modifications. Stock of PB (20 mg/mL) and UDCA (4 mg/mL) were prepared by vortexing the powders with 10% gel3 ,14 ,27 –29 (link). Preparations were mixed for 7 hours, and used within two day of preparation.
Mooranian A., Raj Wagle S., Kovacevic B., Takechi R., Mamo J., Lam V., Watts G.F., Mikov M., Golocorbin-Kon S., Stojanovic G., Al-Sallami H, & Al-Salami H. (2020). Bile acid bio-nanoencapsulation improved drug targeted-delivery and pharmacological effects via cellular flux: 6-months diabetes preclinical study. Scientific Reports, 10, 106.
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10

Preparation and Characterization of Polysaccharides

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Low viscosity sodium alginate and heparin were obtained from Sigma-Aldrich. Chondroitin sulfate and poly-GalA were obtained from Alfa Aesar. Hyaluronic acid, potassium salt from human umbilical cord, and heparin sulfate were obtained from MP biomedicals and sagent pharmaceuticals, respectively. Poly-GlcA was prepared from Avicel PH-105 NF (FMC Biopolymer) and its structure confirmed by 1H NMR as described previously [5 (link)]. Poly-ManA, poly-GulA, and poly-MG were prepared via partial acid hydrolysis of sodium alginate, and structure was confirmed by 1H NMR as described previously [5 (link)]. All prepared polysaccharide samples were dialyzed against 4 L of ddH2O for 40 h at 4 °C with one buffer exchange, lyophilized, and stored as powders at 4 °C until needed.
MacDonald L.C., Weiler E.B, & Berger B.W. (2016). Engineering broad-spectrum digestion of polyuronides from an exolytic polysaccharide lyase. Biotechnology for Biofuels, 9, 43.
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