Octopamine

Manufactured by Thermo Fisher Scientific

Octopamine is a laboratory reagent used in various analytical and biochemical applications. It is a biogenic amine that serves as a neurotransmitter or neuromodulator in some invertebrate organisms. Octopamine is commonly utilized in research settings to study the physiological and behavioral effects of this compound in biological systems.

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Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (3 mentions) Crisprmax, by Thermo Fisher Scientific (2 mentions) Messengermax, by Thermo Fisher Scientific (1 mentions) Opti mem 1 reduced serum medium, by Thermo Fisher Scientific (1 mentions) Rnaimax, by Thermo Fisher Scientific (1 mentions)

2 protocols using octopamine

Transfection of reporter mRNAs in plant-parasitic nematodes

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Capped and polyadenylated mRNAs encoding eGFP or Firefly luciferase were obtained from Ozbiosciences (codon table, UTR sequence, and ribosomal binding site are not disclosed by the supplier). To aid transfection of reporter mRNAs, three lipofection agents were individually used according to the manufacturer’s instructions. Approximately 15,000–20,000 J2 H. schachtii were soaked for 24 h for eGFP and mCherry: in 500 ng of mRNA, 3% lipofectamine RNAIMAX, MessengerMAX, or CRISPRMAX (Invitrogen), 100 mM octopamine (Thermo-Fisher), in a total volume of 50 µl (adjusted with Opti-MEM^™ I Reduced Serum Medium [Invitrogen]). For luciferase assays, 16 µg of mRNA, 12% lipofectamine RNAIMAX, and 100 mM octopamine (Thermo Fisher), were used in a total volume of 30 µl (adjusted with nuclease free M9 buffer).

Kranse O., Beasley H., Adams S., Pires-daSilva A., Bell C., Lilley C.J., Urwin P.E., Bird D., Miska E., Smant G., Gheysen G., Jones J., Viney M., Abad P., Maier T.R., Baum T.J., Siddique S., Williamson V., Akay A, & Eves-van den Akker S. (2021). Toward genetic modification of plant-parasitic nematodes: delivery of macromolecules to adults and expression of exogenous mRNA in second stage juveniles. G3: Genes|Genomes|Genetics, 11(2), jkaa058.

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Capped mRNA Transfection in H. schachtii

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Capped and polyadenylated mRNAs encoding eGFP or Firefly luciferase were obtained from Ozbiosciences (Codon table not disclosed). To aid transfection of reporter mRNAs, two lipofection agents were used according to the manufacturer's instructions. Approximately 15,000 -20,000 J2 H. schachtii were soaked for 24 hours in 12 µg of mRNA, 12 % lipofectamine RNAIMAX or CRISPRMAX (Invitrogen), 100 mM octopamine (Thermo-Fisher), in a total volume of 30 µL (adjusted with nuclease-free M9 buffer). The negative control substituted the mRNA with an equal volume of M9 buffer.

Kranse O.P., Beasley H., Adams S., Pires‐daSilva A., Bell C., Lilley C.J., Urwin P.E., Bird D., Miska E.A., Smant G., Gheysen G., Jones J.T., Viney M., Abad P., Maier T.R., Baum T.J., Siddique S., Williamson V.M., Akay A., & Akker S.E.d. (2020). Towards genetic modification of plant-parasitic nematodes: delivery of macromolecules to adults and expression of exogenous mRNA in second stage juveniles.

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