The expression levels of E-cadherin, VCAM-1, ICAM-1, and CK8/18 were examined by immunohistochemistry in the implanted and metastatic tumor tissues from nude mice and in the surgical specimens used for implantation. Sections used for staining were obtained from the surgical specimens, the implanted and metastatic tumor tissues, and the tissues that contain metastatic tumors. Reagents used for staining were SP-9000 Histostain™-plus Kits, 3-3′-Diaminobenzidine tetrahydrochloride (DAB) Kits, primary mouse monoclonal antibodies against E-cadherin (1:200 dilution), ICAM-1 (1:500 dilution), and primary rabbit polyclonal antibody against VCAM-1 (1:500 dilution) (Beijing Zhongshan Golden Bridge Biotechnology Co Ltd., Beijing, China). The IHC staining slides were independently assessed by two pathologists, and any difference in the decision outcome was resolved by consensus. Staining intensity was assessed as negative, weak, moderate, or strong. The light microscope and image acquisition software were the same as above.
Sp 9000 histostain plus kits
Manufactured by Zhongshan Golden Bridge Biotechnology
Sourced in China
The SP-9000 Histostain™-plus Kits are a suite of immunohistochemistry reagents designed for the detection and visualization of target antigens in tissue samples. The kits provide a reliable and efficient means of performing immunohistochemical staining procedures.
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Lab products found in correlation
2 protocols using sp 9000 histostain plus kits
1
Immunohistochemical Analysis of Cell Adhesion Markers
2
Breast Cancer Microvessel Density and MicroRNA
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Seventy-seven breast cancer tissue samples were obtained from the Department of Pathology of Qilu Hospital of Shandong University from 2011 to 2014. To quantify the microvessel density (MVD), the SP-9000 Histostain™-Plus kits (Zhongshan Goldenbridge Biotechnology Co.) were used to detect CD31 expression following standard steps as previously described (26, 27) .
MicroRNA array analysis. Total RNA was isolated using TRIzol by the manufacturer's protocol. A microarray with 873 miRNA probes was designed in accordance with Sanger miRbase release 12.0. RNA labeling and hybridization were performed as previously described (28) . After hybridization, microarrays were investigated by the LuxScan 10K Microarray Scanner (CapitalBio, Beijing, China), and the images were analyzed by GenePix Pro 6.0 software (Axon Instruments, Foster City, CA, USA). The data are available in the Gene Expression Omnibus (GEO).
Statistical analysis. Statistical software SPSS 18.0 was used. The data are shown as mean ± SD. The difference in statistics was analyzed through the Student's t-test and regarded as statistically significant for P-values <0.05.
MicroRNA array analysis. Total RNA was isolated using TRIzol by the manufacturer's protocol. A microarray with 873 miRNA probes was designed in accordance with Sanger miRbase release 12.0. RNA labeling and hybridization were performed as previously described (28) . After hybridization, microarrays were investigated by the LuxScan 10K Microarray Scanner (CapitalBio, Beijing, China), and the images were analyzed by GenePix Pro 6.0 software (Axon Instruments, Foster City, CA, USA). The data are available in the Gene Expression Omnibus (GEO).
Statistical analysis. Statistical software SPSS 18.0 was used. The data are shown as mean ± SD. The difference in statistics was analyzed through the Student's t-test and regarded as statistically significant for P-values <0.05.
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