Total RNA was isolated from Gingival MSCs using Trizol reagents (Invitrogen, USA) and reverse transcribed into cDNA using PrimeScript™RT reagent Kit with gDNA Eraser (Takara, Dalian, China). The real-time PCR reactions were performed using the SYBR Premix Ex Taq™ II (Takara, Dalian, China) and IcycleriQ Multi-color Real-time PCR Detection System. The primers were synthesized by Sangon Biotech: Sp1 (5′-TGAGACAGCAGGTGGAGAAG-3′, 5′-GGCTCTTCCCTCACTGTCTT-3′); tert (5′-TGCTGGACACTCAGACTTTGGA-3′, 5′-TTCAACCGCAAGACCGACA-3′); GAPDH (5′-TGAAGCAGGCATCTGAGGG-3′, 5′-CGAAGGTGGAAGAGTGGGAG-3′). Primers for miR-21 and U6 are purchased from RiboBio (Guangzhou, China).
Primers for mir 21 and u6
Manufactured by RiboBio
Sourced in China
Primers for miR-21 and U6 are oligonucleotide sequences designed for the detection and quantification of specific RNA molecules, miR-21 and U6, using various molecular biology techniques such as Real-Time PCR and Northern Blotting. These primers provide a tool for researchers to study the expression patterns and functions of these RNA targets.
Automatically generated - may contain errors
Lab products found in correlation
Icycleriq multi color real time pcr detection system, by Takara Bio (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Sybr premix ex taq 2, by Takara Bio (1 mentions)
Primescript rt reagent kit with gdna eraser, by Takara Bio (1 mentions)
Abi prism 7900 ht detection system, by Thermo Fisher Scientific (1 mentions)
Sybr premix dimereraser kit, by Takara Bio (1 mentions)
2 protocols using primers for mir 21 and u6
1
Quantifying Gingival MSC Gene Expression
2
Quantifying Exosomal miRNA Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was isolated from the exosomal pellets, the exosome-depleted supernatant, and whole serum using isothiocyanate-phenol/chloroform extraction procedures. miR-21 expression was examined as previously described [13 ]. Real-time quantitative RT-PCR (qRT-PCR) was performed using SYBR Premix DimerEraser kit (TaKaRa, Shiga, Japan) on an ABI Prism 7900HT Detection System (Applied Biosystems, Foster City, CA). U6 snRNA was used as an internal control. The primers for miR-21 and U6 were purchased from RiboBio (Guangzhou, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!