Rabbit anti bbc3

Antibodies used for flow cytometry were PE‐CD235a (GPA), APC‐CD235a (GPA), PE‐CD34, FITC‐CD36, Percp‐Cy5.5‐7AAD and propidium iodide (PI) (BD Pharmingen); PE‐Cy7‐IL‐3R (CD123) and APC‐α4 integrin (Miltenyi Biotec); PE‐Cy7‐Annexin V (eBioscience); and human band 3 generated in our laboratory and labeled with FITC. Antibodies used for Western blotting were rabbit anti‐U2AF1 from Abcam; rabbit anti‐p53, rabbit anti‐p21, rabbit anti‐BBC3, rabbit anti‐BAX and rabbit anti‐Bcl‐2 from Cell Signalling Technology; anti‐actin antibody from Sigma; HRP‐conjugated mouse anti‐goat IgG from Invitrogen, and HRP‐conjugated goat anti‐rabbit IgG from Thermo Fisher.

The antibodies used for flow cytometry were as follows: mouse monoclonal antibody against human band 3 generated in our laboratory and labeled with FITC or APC as described previously [29 (link)]. Commercial antibodies used for flow cytometry were as follows: PE-CD235a (GPA), PE-CD34, FITC-CD36, and 7AAD (BD Pharmingen, USA); APC-α4 integrin (Miltenyi Biotec, USA); and PE-Cyanine7-IL-3R (CD123) and PE-Cyanine7-Annexin V (eBioscience, USA). The antibodies used for western blotting were as follows: rabbit anti-SF3B1 was from Abcam (USA); rabbit anti-MKRN1 antibody was from BETHYL (USA); rabbit anti-p53, rabbit anti-p21, rabbit anti-BBC3, and rabbit anti-BAX were from Cell Signaling (USA); monoclonal anti-actin antibody was purchased from Sigma (USA); HRP-conjugated goat anti-rabbit IgG was from Thermofisher (USA); and HRP-conjugated mouse anti-goat IgG was from Invitrogen (USA). SYTO-16 green fluorescent was from Invitrogen (USA).
CD34⁺ cell culture, fluorescence-activated cell sorting of erythroblasts, flow cytometry analysis, colony forming assay, cytospin preparation, western blotting analysis, and statistical analysis were performed as previously described [29 (link), 30 ].