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2 protocols using tryptone

1

Spectrophotometric Characterization of Metal Complexes

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Iron(III) chloride (FeCl3), copper(II) nitrate [Cu(NO3)2], cobalt(III) nitrate [Co(NO3)3], zinc acetate [Zn(CH3COO)2], ethanol (C2H5OH), 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH), and 3,5-dinitrosalicyclic acid (DNS) were purchased from Sigma-Aldrich (Singapore), sodium hydroxide (NaOH) from Merck, kanamycin from Bio Basic, and isopropyl-β-D-thiogalactopyranoside from Thermo Fisher Scientific. The growth medium used is Luria-Bertani (LB) medium containing 1% (w/v) tryptone, and 0.5% (w/v) yeast extract was obtained from Liofilchem, 1% (w/v) NaCl from Merck. The composition of the phosphate buffer solution used was Na2HPO4 1 M and NaH2PO4.H2O 1 M, phenol, and H2SO4 97% were from Merck. All the chemicals used for the experiments were of analytical grade. All the solutions were prepared by using deionized water, and the glassware was properly washed and autoclaved before conducting the experiments.
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2

Molecular Diagnostics Protocol Optimization

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Tris, tryptone, yeast extract and casein were purchased from Liofilchem (Italy). Materials required for PCR such as 10X PCR buffer, dNTP (10mM), MgCl 2 (20 mM) and Taq DNA polymerase were purchased from Sigma-Aldrich and other required chemicals from Merck (Germany). The required primers were also synthesized by Bioneer (Korea). The organic solvents were purchased from Merck.
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