Axio observer z1 inverted lsm 880 nlo laser scanning confocal microscope

Manufactured by Zeiss

The Axio Observer Z1 Inverted LSM 880 NLO laser scanning confocal microscope is a high-performance imaging system designed for advanced biological and materials research. It features a laser scanning confocal architecture and is optimized for non-linear optical (NLO) imaging techniques, such as multi-photon excitation. The system provides high spatial and temporal resolution, enabling detailed analysis of complex samples.

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3 protocols using axio observer z1 inverted lsm 880 nlo laser scanning confocal microscope

High-Resolution Confocal Imaging of Insect Tissues

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Images were acquired with a Zeiss Axio Observer Z1 Inverted LSM 880 NLO laser scanning confocal microscope (Zeiss) with either 25x/0.8 NA (whole brains) or 40x/1.4 NA (antennal lobes, antennae) immersion-corrected objective at a resolution of 1024 × 1024 or 2048 × 2048 (brains) or 3024 × 1024 (antennae) pixels. Confocal images were processed in ImageJ (NIH).

Basrur N.S., De Obaldia M.E., Morita T., Herre M., von Heynitz R.K., Tsitohay Y.N, & Vosshall L.B. (2020). Fruitless mutant male mosquitoes gain attraction to human odor. eLife, 9, e63982.

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Confocal Microscopy Imaging Protocol

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Images were acquired with a Zeiss Axio Observer Z1 Inverted LSM 880 NLO laser scanning confocal microscope (Zeiss) with a 25x/0.8 NA immersion-corrected objective at a resolution of 2048 x 2048 or 1024 x 1024 pixels. When necessary, tiled images were stitched with 10% overlap. Confocal images were processed in ImageJ (NIH).

Jové V., Gong Z., Hol F.J., Zhao Z., Sorrells T.R., Carroll T.S., Prakash M., McBride C.S, & Vosshall L.B. (2020). Sensory Discrimination of Blood and Floral Nectar by Aedes aegypti Mosquitoes. Neuron, 108(6), 1163-1180.e12.

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Laser Scanning Confocal Microscopy Protocol

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Images were acquired with a Zeiss Axio Observer Z1 Inverted LSM 880 NLO laser scanning confocal microscope (Zeiss) with either 25x/0.8 NA (whole brains) or 40x/1.4 NA (antennal lobes, antennae) immersion-corrected objective at a resolution of 1024 x 1024 or 2048 x 2048 (brains) or 3024 x 1024 (antennae) pixels. Confocal images were processed in ImageJ (NIH).

Basrur N.S., Obaldia M.E.D., Morita T., Herre M., Heynitz R.K.v., Tsitohay Y.N., & Vosshall L.B. (2020). fruitless mutant male mosquitoes gain attraction to human odor.

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