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Echomri 3 in 1 v2

Manufactured by Echo Medical Systems
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The EchoMRI™ 3-in-1 v2.1 is a multi-modal imaging system that combines Magnetic Resonance Imaging (MRI), Computed Tomography (CT), and Positron Emission Tomography (PET) capabilities in a single integrated platform. It is designed to provide comprehensive diagnostic imaging solutions for healthcare facilities.

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EchoMRI 3-in-1 EchoMRI 3-1 The 3-in-1 EchoMRI

5 protocols using echomri 3 in 1 v2

1

Comprehensive Body Composition and Metabolism Analysis

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Body composition (total fat and lean mass), locomotor activity, and indirect calorimetry measurements were performed at the UAB Animal Physiology Core. Total fat and lean mass were assessed in unanesthetized mice using a noninvasive quantitative magnetic resonance imaging system (EchoMRI™ 3-in-1 v2.1; Echo Medical Systems, Houston, TX, USA), as previously reported [28 (link)]. Percent body fat and lean mass were calculated as [fat mass (or lean mass)/body weight] × 100.
Resting energy expenditure (REE), activity, and food intake were quantified using an eight-cage CaloSys indirect calorimetry system (TSE Systems, Inc., Chesterfield, MO, USA). To acclimate to the new environment, mice were individually kept in metabolic cages with ad libitum access to food and water for 48 h before measurements. Subsequently, O2 consumption, CO2 production, food intake, and spontaneous locomotor activity were continuously measured for 24 h. REE was determined as the average of the three lowest 18 min-intervals, with at least 1 h in between intervals, as previously reported [29 (link)]. Locomotor activity was monitored by a multidimensional infrared light beam system surrounding each cage.
De Luca M., Vecchie’ D., Athmanathan B., Gopalkrishna S., Valcin J.A., Swain T.M., Sertie R., Wekesa K., Rowe G.C., Bailey S.M, & Nagareddy P.R. (2019). Genetic Deletion of Syndecan-4 Alters Body Composition, Metabolic Phenotypes, and the Function of Metabolic Tissues in Female Mice Fed A High-Fat Diet. Nutrients, 11(11), 2810.
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2

Long-Term Obesity Phenotype Study

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Body weight was measured weekly for the first month and every four weeks thereafter until 43 weeks of age (unless identified, all week numerals indicate mice age). Food was changed biweekly and measured every two of four weeks' interval. Food intake was obtained by subtracting remaining food, including any spilled food in cages, from a weighed aliquot for a period of two weeks. Energy intake was calculated on the basis of 4.73kcal/g for the HFD and 3.85kcal/g for the LFD (caloric values obtained from Research Diets, Inc.). Body composition (fat and fat-free mass) was determined in vivo using quantitative magnetic resonance (QMR) (EchoMRI™ 3-in-1 v2.1; Echo Medical Systems, Houston, TX) at 8, 23 and 43 weeks of age as described (14 (link)). Caloric content of the change in body mass was computed at 1.8 kcal/g for fat-free tissue and 8 kcal/g for fat tissue (15 (link)).
Moribund mice, including mice with ulcerative dermatitis or similar skin lesions (n=29) or those unable to eat/drink (one female), were euthanized in accordance with university and IACUC policies. One male mouse died spontaneously during the study. A total of 23 female and 8 male mice were dead or removed prior to 10 months of age; thus, 554 mice were included in the data analysis (255 HFD females, 269 HFD males, 15 LFD females and 15 LFD males).
Yang Y., Smith DL J.r., Keating K.D., Allison D.B, & Nagy T.R. (2014). Variations in body weight, food intake and body composition after long-term high-fat diet feeding in C57BL/6J Mice. Obesity (Silver Spring, Md.), 22(10), 2147-2155.
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3

Long-Term Obesity Phenotype Study

Cited 1 time
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Body weight was measured weekly for the first month and every four weeks thereafter until 43 weeks of age (unless identified, all week numerals indicate mice age). Food was changed biweekly and measured every two of four weeks' interval. Food intake was obtained by subtracting remaining food, including any spilled food in cages, from a weighed aliquot for a period of two weeks. Energy intake was calculated on the basis of 4.73kcal/g for the HFD and 3.85kcal/g for the LFD (caloric values obtained from Research Diets, Inc.). Body composition (fat and fat-free mass) was determined in vivo using quantitative magnetic resonance (QMR) (EchoMRI™ 3-in-1 v2.1; Echo Medical Systems, Houston, TX) at 8, 23 and 43 weeks of age as described (14 (link)). Caloric content of the change in body mass was computed at 1.8 kcal/g for fat-free tissue and 8 kcal/g for fat tissue (15 (link)).
Moribund mice, including mice with ulcerative dermatitis or similar skin lesions (n=29) or those unable to eat/drink (one female), were euthanized in accordance with university and IACUC policies. One male mouse died spontaneously during the study. A total of 23 female and 8 male mice were dead or removed prior to 10 months of age; thus, 554 mice were included in the data analysis (255 HFD females, 269 HFD males, 15 LFD females and 15 LFD males).
Yang Y., Smith DL J.r., Keating K.D., Allison D.B, & Nagy T.R. (2014). Variations in body weight, food intake and body composition after long-term high-fat diet feeding in C57BL/6J Mice. Obesity (Silver Spring, Md.), 22(10), 2147-2155.
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4

Longitudinal Body Composition Analysis

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From 47 weeks of age, body composition (fat and fat‐free mass) was determined in vivo in all groups by quantitative magnetic resonance (EchoMRI 3‐in‐1, V2.1; Echo Medical Systems, Houston, Texas) at peak and trough points of the WC group (~3‐4 month intervals). The specific week of measurement differed between male and female mice to allow the time needed to reach the targeted weight or to stagger measurements to accommodate the number of animals.
Smith DL J.r., Yang Y., Nagy T.R., Patki A., Vasselli J.R., Zhang Y., Dickinson S.L, & Allison D.B. (2018). Weight Cycling Increases Longevity Compared with Sustained Obesity in Mice. Obesity (Silver Spring, Md.), 26(11), 1733-1739.
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5

Body Composition Analysis via EchoMRI

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Body composition was assessed through EchoMRI Analysis (EchoMRI 3-in-1 v2.1; Echo Medical Systems, Houston, TX), that accurately measures lean, fat, free water and total water masses in live animals. Mice were scanned 12 hours prior to being euthanized. Animals did not receive anesthesia nor special preparation before measurement. Scanning took up to 5 minutes per mouse.
Calore F., Londhe P., Fadda P., Nigita G., Casadei L., Marceca G.P., Fassan M., Lovat F., Gasparini P., Rizzotto L., Zanesi N., Jackson D., Mehta S., Nana-Sinkam P., Sampath D., Pollock R.E., Guttridge D.C, & Croce C.M. (2018). The TLR7/8/9 antagonist IMO-8503 inhibits cancer-induced cachexia. Cancer research, 78(23), 6680-6690.
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