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Rabbit anti egfr

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Rabbit anti-EGFR is a laboratory reagent used for the detection and quantification of epidermal growth factor receptor (EGFR) in various biological samples. It is a polyclonal antibody raised in rabbits and specifically binds to the EGFR protein.

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Lab products found in correlation

Mouse anti transferrin receptor, by Thermo Fisher Scientific (2 mentions) Duolink in situ red starter kit goat rabbit, by Merck Group (2 mentions) Rabbit anti akt, by Cell Signaling Technology (1 mentions) Rabbit anti erk1 2, by Cell Signaling Technology (1 mentions) Rabbit anti p erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions) Rabbit anti pegfr tyr1068, by Cell Signaling Technology (1 mentions) Rabbit anti p akt ser473, by Cell Signaling Technology (1 mentions) Rabbit anti rab7, by Cell Signaling Technology (1 mentions) Rabbit anti cathepsin d, by Abcam (1 mentions) Mouse anti eea1, by Santa Cruz Biotechnology (1 mentions) Rabbit anti ha, by Cell Signaling Technology (1 mentions) Mouse anti ha ha 11 clone 16b12, by Fortrea (1 mentions)

Close spelling variants of this product

Rabbit polyclonal anti-EGFR antibody (2 citations) Anti-EGFR (21 citations) Rabbit anti- (2 citations)

4 protocols using rabbit anti egfr

1

Comprehensive Immunoblot Antibody Panel

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Mouse anti-HA HA.11 clone 16B12 (#MMS-101P, Covance, 1:1000). Goat anti-BCA2 (#SAB2500854, Sigma Aldrich, 1:500). Rabbit anti-EGFR (#HPA018530, Sigma, 1:1000). Mouse anti-Transferrin Receptor (#13-6800, Life Technologies, 1:500). Mouse anti-c-Met (#3127, Cell Signalling, 1:500). Rabbit anti-pEGFR Tyr1068 (#3777, Cell Signalling, 1,000). Rabbit anti-AKT (#9272, Cell Signalling, 1:1,000). Rabbit anti-pAKT Ser473 (#9271, Cell Signalling, 1:500). Rabbit anti-ERK 1/2 (#9102, Cell Signalling, 1:500). Rabbit anti-pERK 1/2 Thr202/Tyr204 (#9101, Cell Signalling, 1:500). Mouse HRP conjugated anti-β-tubulin (#ab21058, Abcam, 1:50,000). All secondary antibodies for Western blotting were from Pierce and were used at a dilution of 1:2000.
Wymant J.M., Hiscox S., Westwell A.D., Urbé S., Clague M.J, & Jones A.T. (2016). The Role of BCA2 in the Endocytic Trafficking of EGFR and Significance as a Prognostic Biomarker in Cancer. Journal of Cancer, 7(15), 2388-2407.
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2

Proximity Ligation Assay for EGFR Interaction

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Sections from fresh-frozen brains were fixed in 4% paraformaldehyde for 15 min, permeabilized and blocked with TritonX-100 (0.3%) and 5% Horse Serum/PBS for 1 h. Sections were incubated with XPA (Xoma Laboratories) and rabbit anti-EGFR (Sigma, Ab-1070) at 4 °C overnight. PLA was performed using a Duolink® In Situ Red Starter Kit Goat/Rabbit (Sigma-Aldrich) according to manufacturer’s instructions. To assess signal specificity, only one primary antibody was used.
Duquenne M., Folgueira C., Bourouh C., Millet M., Silva A., Clasadonte J., Imbernon M., Fernandois D., Martinez-Corral I., Kusumakshi S., Caron E., Rasika S., Deliglia E., Jouy N., Oishi A., Mazzone M., Trinquet E., Tavernier J., Kim Y.B., Ory S., Jockers R., Schwaninger M., Boehm U., Nogueiras R., Annicotte J.S., Gasman S., Dam J, & Prévot V. (2021). Leptin brain entry via a tanycytic LepR:EGFR shuttle controls lipid metabolism and pancreas function. Nature metabolism, 3(8), 1071-1090.
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3

Proximity Ligation Assay for EGFR Interaction

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Sections from fresh-frozen brains were fixed in 4% paraformaldehyde for 15 min, permeabilized and blocked with TritonX-100 (0.3%) and 5% Horse Serum/PBS for 1 h. Sections were incubated with XPA (Xoma Laboratories) and rabbit anti-EGFR (Sigma, Ab-1070) at 4 °C overnight. PLA was performed using a Duolink® In Situ Red Starter Kit Goat/Rabbit (Sigma-Aldrich) according to manufacturer’s instructions. To assess signal specificity, only one primary antibody was used.
Duquenne M., Folgueira C., Bourouh C., Millet M., Silva A., Clasadonte J., Imbernon M., Fernandois D., Martinez-Corral I., Kusumakshi S., Caron E., Rasika S., Deliglia E., Jouy N., Oishi A., Mazzone M., Trinquet E., Tavernier J., Kim Y.B., Ory S., Jockers R., Schwaninger M., Boehm U., Nogueiras R., Annicotte J.S., Gasman S., Dam J, & Prévot V. (2021). Leptin brain entry via a tanycytic LepR:EGFR shuttle controls lipid metabolism and pancreas function. Nature metabolism, 3(8), 1071-1090.
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4

Immunofluorescence Staining of Organelles

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Mouse anti-HA HA.11 clone 16B12 (#MMS-101P, Covance, 1:1000). Rabbit anti-EGFR
(#HPA018530, Sigma, 1:100). Mouse anti-EEA1 (#SC-6415, Santa-Cruz, 1:200). Rabbit anti-LAMP2 (#H4B4, DSHB, 1:200). Rabbit anti-Rab7 (#9367, Cell Signalling, 1:100). Rabbit anti-HA (#3724, Cell Signalling, 1:1600). Rabbit anti-cathepsin D (#ab72915, Abcam, 1:100). Mouse anti-transferrin receptor (#13-6800, Life Technologies, 1:300). All secondary antibodies for IF were from Life Technologies and were used at a dilution of 1:400.
Wymant J.M., Hiscox S., Westwell A.D., Urbé S., Clague M.J, & Jones A.T. (2016). The Role of BCA2 in the Endocytic Trafficking of EGFR and Significance as a Prognostic Biomarker in Cancer. Journal of Cancer, 7(15), 2388-2407.
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