Anti fadd 556402

KillerTRAIL (human recombinant) was purchased from Alexis Biochemicals. The nine most highly selective molecules targeting cFLIP were obtained from NCI–DSCB (National Cancer Institute-Drug Synthesis and Chemistry Branch, Rockville, MD, USA). For Western blotting (WB) experiments, we used the following antibodies: anti-FLIP antibodies DAVE2 and NF6 (Adipogen, San Diego, CA, USA), caspase-3 (8G10; OZYME), PARP (Asp214, 19F4; OZYME, Saint-Cyr-l’École, France), caspase-8 (1C12; OZYME), anti-MBP (New England Biolabs, Ipswich, MA, USA) and anti-His (C-ter) (Invitrogen, Carlsbad, CA, USA). Anti-His (ab81663) was used to immunoprecipitate the DISC complex, and the following antibodies were used for WB analysis: anti-Flip (DAVE II, Adipogen), anti-FADD (556402, BD, San Jose CA, USA), anti-casp8 (5F7, EnzoLife, Farmingdale, NY, USA), anti-DR4 (1139, ProSci, Poway, CA, USA) and anti-DR5 (3696, Cell Signaling, Danvers, MA, USA). Anti-rabbit and anti-mouse HRP-linked secondary antibodies (Santa Cruz Biotechnology, Dallas, TX, USA) and β-actin (Sigma Aldrich, St. Louis, MO, USA) were also used.

The MEK inhibitor U0126 was purchased from LC Laboratories (Woburn, MA). The JNK inhibitor SP600125 was purchased from Enzo Life Sciences, Inc./Biomol (Farmingdale, NY). Rabbit polyclonal anti-DR5 antibody was purchased from ProSci Inc. (Poway, CA). Mouse monoclonal anti-DR4 antibody (B-N28) was purchased from Diaclone (Stamford, CT). Protein-A/G plus-agarose, rabbit polyclonal anti-TRAF2 (sc-7187) and Fra-1 antibodies were purchased from Santa Cruz Biotechnology, Inc (Santa Cruz, CA). Rabbit polyclonal antibody against HA tag was purchased from Abgent (San Diego, CA). Mouse monoclonal anti-MMP1 antibody was purchased from NeoMarkers, Inc. (Union City, CA). Antibodies against actin and Flag-tag and anti-Flag M2 affinity gel were purchased from Sigma-Aldrich (St. Louis, MO). Mouse monoclonal anti-TRAF2 (#558890) and anti-FADD (#556402) antibodies were purchased from BD Biosciences (San Jose, CA). Mouse monoclonal anti-caspase-8 (#9746), rabbit monoclonal anti-caspase-8 (#4790), rabbit polyclonal ant-FADD (#2782) and all other antibodies were all purchased from Cell Signaling Technology, Inc. (Beverly, MA). Anti-HA matrix agarose was purchased from Thermo Scientific/Pierce (Rockford, IL).