Tissues were fixed in 10% neutral formalin overnight at room temperature and dehydrated to make wax blocks. Paraffin sections (3 µm) were deparaffinized, dehydrated and subsequently subjected to antigen retrieval using citrate buffer. Deparaffinized sections were blocked with 10% goat serum (Fuzhou Maixin Biotech Co., Ltd.) for 15 min at room temperature, and incubated with 3% H2O2 for 15 min at room temperature to inhibit endogenous peroxidase activity and non-specific antigens. Sections were incubated with anti-p38 MAPK (1:250; ProteinTech Group, Inc.; cat. no. 66234-1-Ig) at 4°C for 16 h and HRP-conjugated secondary antibody (1:200; ProteinTech Group, Inc.; cat. no. SA00001-1) for 30 min at room temperature. DAB substrate was applied to assess signal detection and observed under a light microscope (×400 magnification). Staining intensity was assessed, as previously described (27 (link)).
Anti p38 mapk
Manufactured by Proteintech
Sourced in China
Anti-p38 MAPK is a laboratory reagent used for the detection and quantification of p38 MAPK, a member of the mitogen-activated protein kinase (MAPK) family. It is a specific antibody that binds to p38 MAPK, enabling its identification and measurement in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti erk1 2, by Proteintech (2 mentions)
Hrp conjugated secondary antibody, by Proteintech (1 mentions)
Anti p jnk sc 6254, by Santa Cruz Biotechnology (1 mentions)
Anti p p38 sc 7973, by Santa Cruz Biotechnology (1 mentions)
Anti ub sc 8017, by Santa Cruz Biotechnology (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti cleaved parp, by Cell Signaling Technology (1 mentions)
Anti p62 sqstm1, by Proteintech (1 mentions)
Anti p ampk thr172, by Cell Signaling Technology (1 mentions)
Anti β actin, by Proteintech (1 mentions)
Anti ha tag, by Cell Signaling Technology (1 mentions)
Anti p erk1 2, by Cell Signaling Technology (1 mentions)
Anti akt, by Proteintech (1 mentions)
Anti jnk, by Proteintech (1 mentions)
Anti flag tag, by Proteintech (1 mentions)
Anti nrf2, by Proteintech (1 mentions)
Anti lc3, by Proteintech (1 mentions)
Anti mtor, by Proteintech (1 mentions)
Anti keap1, by Proteintech (1 mentions)
Anti phospho akt ser473, by Proteintech (1 mentions)
4 protocols using anti p38 mapk
1
Immunohistochemical Analysis of p38 MAPK
2
Western Blot Analysis of Cellular Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total protein separation and western blotting were performed as described previously [16 (link)]. Western blot analysis was performed using anti-cleaved PARP (#5625), anti-cleaved caspase-3 (#9661), anti-p-AMPK (Thr172) (#50081), anti-p-ERK1/2 (#4370), and anti-HA-Tag (#3724) antibodies purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-LC3 (14600–1-AP), anti-P62/SQSTM1 (18420–1-AP), anti-phospho-Akt (Ser-473) (66444–1-Ig), anti-Akt (10176–2-AP), anti-mTOR (20657–1-AP), anti-P38 MAPK (14064–1-AP), anti-JNK (51151–1-AP), anti-ERK1/2 (16443–1-AP), anti-AMPK (10929–2-AP), anti-Nrf2 (16396–1-AP), anti-Keap1 (10503–2-AP), anti-FLAG-tag (66008–2-AP), anti-MYC-tag (60003–2-AP), and anti-β-actin (66009–1-Ig) antibodies were obtained from Proteintech Group Co., Ltd. (Wuhan, China). An anti-phospho-mTOR (Ser-2448) (ab109268) antibody was purchased from Abcam (Cambridge, MA, USA). Anti-p-p38 (sc-7973), anti-p-JNK (sc-6254), and anti-Ub (sc-8017) antibodies were obtained from Santa Cruz Biotechnology., Inc. (Santa Cruz, CA, USA).
3
Western Blot Antibody Validation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Western blotting assay were performed as described previously (Zang et al. 2022 (link)). The antibodies used were as follows: anti-MCCC2 (12117-1-AP, Proteintech, China); anti-ECHDC2 (bs-13049R, Bioss, China); anti-GLUT1 (21829-1-AP, Proteintech, China); anti-PKM2 (15822-1-AP, Proteintech, China); anti-SDHA (14865-1-AP, Proteintech, China); anti-G6PD (ab993, Abcam, UK); anti-c-Myc (10828-1-AP, Proteintech, China); anti-p-AKT (66444-1-lg, Proteintech, China); anti-P38 MAPK (14064-1-AP, Proteintech, China); anti-ubiquitin (10201-2-AP, Proteintech, China); anti-NEDD4 (21698-1-AP, Proteintech, China); anti-ITCH (20920-1-AP, Proteintech, China); anti-CBL (25818-1-AP, Proteintech, China); anti-GAPDH (10494-1-AP, Proteintech, China).
4
Protein Expression Analysis via SDS-PAGE
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SDS polyacrylamide gel electrophoresis tests were performed as the previous study [25 (link)]. Protein expression levels were detected by ECL luminescence imaging. The primary antibodies used in this experiment included anti-PRDM9(PA541161;Invitrogen); anti-FBLN5(Catalog # 3095-FB; R&D system); anti-p16 (Cat No. 10883-1-AP;proteintech), anti-p53(Cat No. 60283-2-Ig; proteintech), anti-phospho-p38 MAPK(Cat No. 28796-1-AP ;Proteintech), anti-p38MAPK(Cat No. 14064-1-AP ;Proteintech), anti-phospho-Erk1/2(Cat No: 80031-1-RR;Proteintech), anti-Erk1/2(CatNo. 11257-1-AP; Proteintech); anti-phospho-JNK (CatNo. 4668; Cell Signaling Technology), anti‐JNK (Cat No. 9258; Cell Signaling Technology) and anti-GAPDH (Cat No. 60004-1-1g; Proteintech)
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!