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Amplicor monitor standard assay cobas ampliprep cobas taqman hiv test

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The Roche Amplicor monitor standard assay (COBAS AmpliPrep/COBAS TaqMan HIV Test) is a laboratory diagnostic tool used for the quantitative detection of HIV-1 ribonucleic acid (RNA) in human plasma.

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Lab products found in correlation

Facscalibur flow cytometer, by BD (2 mentions) Trucount tube, by BD (2 mentions)

Spelling variants of this product

COBAS AmpliPrep/COBAS TaqMan (63 citations) COBAS AmpliPrep/COBAS TaqMan assay (13 citations) Amplicor HIV Monitor Test (7 citations) COBAS AmpliPrep/TaqMan (7 citations) Standard Amplicor HIV Monitor assay (4 citations) Amplicor HIV Monitor Assay (4 citations) Standard Amplicor HIV Monitor (3 citations) Cobas Amplicor HIV Monitor Test (2 citations) Cobas TaqMan/Cobas Ampliprep HIV-test (2 citations) COBAS ® AMPLICOR Monitor test (2 citations)

2 protocols using amplicor monitor standard assay cobas ampliprep cobas taqman hiv test

1

Quantifying CD4+ T Cells and HIV RNA

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The total count of CD4+ T cells in whole blood was determined using the FACSCalibur flow cytometer (BD Biosciences, NJ, USA). The standard lyse/no-wash procedure was used with anti-CD4-fluorescein isothiocyanate (FITC)/CD8-phycoerythrin (PE)/CD3-peridinin chlorophyll protein (PerCP) as staining reagents and TruCount tubes (BD Biosciences, NJ, USA). TruCount control beads were used for quality control. The Roche Amplicor monitor standard assay (COBAS AmpliPrep/COBAS TaqMan HIV Test; Roche, Switzerland) was used to detect HIV RNA in plasma. The values were calculated according to the manufacturer’s reference standards. The limit of detection was set at 20 copies/mL.
Ding H., Zhang X., Zhu Z., Wang S., Xiong Y., Shang H, & Liang G. (2022). Membrane Protein OTOF Is a Type I Interferon-Induced Entry Inhibitor of HIV-1 in Macrophages. mBio, 13(4), e01738-22.
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2

CD4+ T-cell Enumeration and HIV Viral Load

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CD4+ T-cell counts were measured from whole blood and performed using the FACSCalibur Flow Cytometer (BD Biosciences, NJ, USA). The standard lyse/no-wash procedure was used with anti-CD4-FITC/CD8-PE/CD3-PerCP as staining reagents and TruCOUNT tubes (BD Biosciences, NJ, USA). TruCOUNT Control Beads were used for quality control. A Roche Amplicor Monitor Standard Assay (COBAS AmpliPrep/COBAS Taqman HIV Test; Roche, Switzerland) was used to detect plasma HIV RNA. The values were calculated according to the manufacturer's reference standards. The limit of detection was set at 20 copies/mL.
Zhao L., Liu M., Ouyang J., Zhu Z., Geng W., Dong J., Xiong Y., Wang S., Zhang X., Qiao Y., Ding H., Sun H., Liang G., Shang H, & Han X. (2018). The Per-1 Short Isoform Inhibits de novo HIV-1 Transcription in Resting CD4+ T-cells. Current HIV Research, 16(6), 384-395.
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