Anti lamin a c sc 7292

Western blot was performed as previously described (Jian et al., 2011) using the following antibodies: anti-K17 (ab53707; Abcam), anti-FASN (ab128856; Abcam), anti-SREBP-1 (PA1-337; Invitrogen), anti-PPARγ (81B8; Cell Signaling Technology), anti-β-tubulin (10068-1-AP; Proteintech), anti-β-actin (66009-1-Ig, Proteintech), anti-GAPDH (60004--1-Ig; Proteintech), and anti-lamin-A/C (sc-7292; Santa Cruz Biotechnology). Band intensities were quantified using Image Lab version 5.2.1 (Bio-Rad). Relative band intensities were normalized to that of the loading control.

Nuclear and cytoplasmic fractions were collected using the ProteoExtract Subcellular Proteome Extraction Kit (Calbiochem, San Diego, CA, USA) and analyzed by immunoblotting assay using the following antibodies: anti-NF-κB p65 (#8242), anti-phospho-JNK (#9251), anti-JNK (#9252), anti-phospho-p38MAPK (#9211), anti-p38MAPK (#9212), phospho-ERK1/2 (#9101), anti-ERK1/2 (#9102), anti-phospho-Akt (#9271), anti-Akt (#9272), anti-XIAP (#2042), and anti-Survivin (#2803) (Cell Signaling Technology, Beverly, MA, USA); anti-Bcl-xL (sc-8392), anti-Bcl-2 (sc-7382), anti-Bax (sc-7480), anti-Bim (sc-374358), anti-caspase 3 (sc-7272), and anti-Lamin A/C (sc-7292) (Santa Cruz Biotechnologies, CA, USA); and anti-β-actin (A2228, Sigma, St Louis, MO, USA). The amount of detected protein was measured via densitometry using a CS analyzer (ATTO, Tokyo, Japan), and detected proteins were standardized to corresponding proteins.