Indirect immunofluorescence was performed as described previously. Anti-Cx26 mAb clone CX-12H10 (Thermo Fisher Scientific) and anti-p120 Catenin pAb (Sigma-Aldrich, St. Louis, MO, USA), anti-GM130 pAb (Sigma-Aldrich) were diluted at 1:150, 1:200, and 1:3500, respectively. After fixation with acetone, cells are incubated with the diluted primary antibodies. Specific signals were revealed by anti-mouse IgG-Alexa 488 (Thermo Fisher Scientific) and anti-rabbit IgG-Alexa 568 (Thermo Fisher Scientific). Nuclei were stained with DAPI (KPL, Gaithersburg, MD, USA) at a concentration of 0.5 µg/mL.
Anti cx26 mab clone cx 12h10
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-Cx26 mAb clone CX-12H10 is a monoclonal antibody that specifically binds to the connexin 26 (Cx26) protein. Connexin 26 is a gap junction protein that plays a role in cell-to-cell communication. This antibody can be used for the detection and study of Cx26 in various applications.
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Lab products found in correlation
2 protocols using anti cx26 mab clone cx 12h10
1
Immunofluorescence Staining of Cx26, p120, and GM130
2
IHC of Connexin 26 in FFPE Samples
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Formalin fixed paraffin-embedded sections on slide glass were deparaffinised, then immersed in 3% hydrogen peroxide/methanol at room temperature for 15 min. The slides were incubated with anti-Cx26 mAb clone CX-12H10 (Thermo Fisher Scientific) in a humidified chamber at 4 °C overnight. Specific signals were visualised by employing HRP-labelled polymer method as follows, the slides were reacted with EnVision+ system-HRP for mouse (Agilent, Santa Clara, CA, USA) at room temperature for 30 min and finally 3,3′-diaminobenzidine was oxidized for signal detection.
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