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Arf4 11673 1 ap

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ARF4 (11673-1-AP) is an antibody that specifically targets the ARF4 (ADP-ribosylation factor 4) protein. ARF4 is a member of the ADP-ribosylation factor (ARF) family of small GTPases, which play important roles in regulating intracellular vesicular trafficking. This antibody can be used to detect and study the expression and localization of ARF4 in various biological samples.

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Lab products found in correlation

Chemidoc system, by Bio-Rad (2 mentions) Cleaved caspase 3 9664, by Cell Signaling Technology (2 mentions) Parp 9542, by Cell Signaling Technology (2 mentions) Hnrnp e1, by Abnova (2 mentions) Hsp90 sc 13119, by Santa Cruz Biotechnology (2 mentions) Ab24359, by Abcam (2 mentions) E cadherin 3195, by Abcam (2 mentions) Ab102470, by Abcam (2 mentions) Bbf2h7 creb3l2 mabe1018, by Merck Group (2 mentions)

2 protocols using arf4 11673 1 ap

1

Comprehensive Protein Expression Analysis

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Cells were lysed in RIPA buffer and protein concentrations were determined prior to Laemmli sample buffer addition and heat denaturation at 95°C for 5 min. Samples were resolved on SDS–PAGE gels and transferred to PVDF membrane. Blots were probed with antibodies specific to COPB1 (ab24359) and USO1 (ab102470) from Abcam, E-cadherin (3195), DYKDDDDK Tag (14793), Chop (2895), cleaved caspase-3 (9664) and PARP (9542) from Cell Signaling Technology, LZIP/CREB3 (sc515434), GAPDH (sc32233) and Hsp90 (sc13119) from Santa Cruz Biotechnology, hnRNP E1 (M01; Abnova), ARF4 (11673-1-AP; Proteintech), PERK (100-401-962; Rockland), BBF2H7/CREB3L2 (MABE1018: Millipore) and V5 (R96025: ThermoFisher). Chemiluminescence was detected by CCD camera (BioRad ChemiDoc system).
Howley B.V., Link L.A., Grelet S., El-Sabban M, & Howe P.H. (2017). A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer. Oncogene, 37(10), 1308-1325.
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2

Comprehensive Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in RIPA buffer and protein concentrations were determined prior to Laemmli sample buffer addition and heat denaturation at 95°C for 5 min. Samples were resolved on SDS–PAGE gels and transferred to PVDF membrane. Blots were probed with antibodies specific to COPB1 (ab24359) and USO1 (ab102470) from Abcam, E-cadherin (3195), DYKDDDDK Tag (14793), Chop (2895), cleaved caspase-3 (9664) and PARP (9542) from Cell Signaling Technology, LZIP/CREB3 (sc515434), GAPDH (sc32233) and Hsp90 (sc13119) from Santa Cruz Biotechnology, hnRNP E1 (M01; Abnova), ARF4 (11673-1-AP; Proteintech), PERK (100-401-962; Rockland), BBF2H7/CREB3L2 (MABE1018: Millipore) and V5 (R96025: ThermoFisher). Chemiluminescence was detected by CCD camera (BioRad ChemiDoc system).
Howley B.V., Link L.A., Grelet S., El-Sabban M, & Howe P.H. (2017). A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer. Oncogene, 37(10), 1308-1325.
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