Heat inactivated fetal bovine serum

Manufactured by PAN Biotech

Sourced in Germany, Belgium

Heat-inactivated fetal bovine serum is a cell culture supplement derived from the blood of fetal bovines. It is processed by heat-inactivation to inactivate any potential biological contaminants.

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Lab products found in correlation

4 protocols using heat inactivated fetal bovine serum

Spodoptera frugiperda Cell Lines for Baculovirus Expression

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Example 7

The Spodoptera frugiperda Sf21 or SP cell lines were cultured in 6-well tissue culture plates (1×10⁶cells/well) in TNM-FH insect medium (Pan Biotech™, Germany) containing 10% heat-inactivated fetal bovine serum (Pan Biotech™, Germany) at 27° C. AcMNPV recombinant baculoviruses were obtained by the “Bac-to-Bac” Baculovirus Expression System (Invitrogen™, USA). The different TB(+) transfer vectors containing the recombinant DNA regulatory elements were generated using the pFastBac™-DUAL plasmid (Invitrogen™). These transfer vectors were used to transfect Sf21 cells with Cellfectin® (Invitrogen™, USA). The resulting recombinant baculoviruses from the infection of Sf21 cells were then passaged twice in cells and titered by the plaque assay method. The obtained gene constructs of the TB (+) baculovirus expression cassettes are schematically shown in FIGS. 4, 7 and 10, showing the combinations of genetic regulatory elements involved in the genes expression (polhAc-ie-01/hr1p6.9p10, SEQ ID NO.: 17, plus the sequence of the gene coding for the desired protein, e.g., SEQ ID NOs: 26, 30, 32 and 33). The different expression cassettes were used to generate the recombinant baculoviruses used in the examples shown in FIGS. 6, 8, 9, 11, 12, 13, and 14.

US11278014B2. Expression of recombinant proteins in Trichoplusia ni pupae (2022-03-22). Alternative Gene Expression, S.L. [ES]. Inventors: José Ángel Martínez Escribano [ES], Carmen Alvarado Fradua [ES], Edel Reytor Saavedra [ES], Miguel Cid Fernandez [ES].

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Porcine Cell Culture and Porcine Deltacoronavirus Isolation

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LLC-PK1 cells, a porcine kidney cell line, were purchased from ATCC and subsequently cultured in Dulbecco’s modified Eagle’s medium (Gibco) containing 10% heat-inactivated fetal bovine serum (PAN-biotech) and a combination of penicillin and streptomycin (Solarbio), in a humidified incubator with an atmosphere of 5% CO₂ at 37 °C. PDCoV strain CH/JXJGS01/2016 (GenBank accession number KY293677.1) was isolated in our laboratory in 2016 from a newborn piglet with diarrhea. Mouse mAb against β-actin and hemagglutinin (HA) were bought from Medical and Biological Laboratories (Japan). Rabbit anti-B23 antibody was purchased from Proteintech (CHI, USA). Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (H + L) was bought from antGene (China). FITC-conjugated goat anti-mouse IgG(H + L) and Alexa Fluor® 594 donkey anti-rabbit IgG(H + L) were bought from and TransGen Biotech (China). All enzymes used for the cloning procedures were purchased from Takara (Dalian, China). Freund’s complete/incomplete adjuvants, polyethylene glycol 1450 and HT/HAT medium were purchased from Sigma-Aldrich (MO, USA).

Ding Z., Luo S., Gong W., Wang L., Ding N., Chen J., Chen J., Wang T., Ye Y., Song D., Kong L., Zhang J, & Tang Y. (2020). Subcellular localization of the porcine deltacoronavirus nucleocapsid protein. Virus Genes, 56(6), 687-695.

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Primary Culture of Salivary Gland Epithelial Cells

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Primary cultures of SGECs from pSS and controls were established from MSGBs as previously described [26] (link). After 2-3 weeks of culture, cells at 70-80% conuence were dissociated with 0.125% trypsin-EDTA. Then, cells were suspended in basal epithelial medium, seeded at 80 000 cells/cm 2 to a 6-well plate coated with collagen type I (Institut de Biotechnologies, Reims, France), and incubated at 37°C and 5% CO 2 in a humidied atmosphere. The basal epithelial medium was changed at day 1 to remove non-adherent SGECs. When SGECs reached 70% confluency, we added the B cells (isolated as described above). The coculture lasted 5 days in 2 mL RPMI-1640 (Lonza, Verviers, Belgium) supplemented with 10% heat-inactivated fetal bovine serum (PAN biotech, Aidenbach, Germany) and 100 Units/mL penicillin and 100 Units/mL streptomycin (Lonza, Verviers, Belgium). After 5 days, B lymphocytes were harvested, SGECs were washed twice with PBS, and then harvested and frozen (-80°C).

Rivière E., Chivasso C., Pascaud J., Bechara R., Ly B., Delporte C., Mariette X, & Nocturne G. (2023). Hyperosmolar environment and salivary gland epithelial cells increase extra-cellular matrix remodeling and lymphocytic infiltration in Sjögren's syndrome. Clinical and experimental immunology, 212(1).

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Molecular Mechanisms of Cardiomyoblast Regulation

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H9C2 cardiomyoblast cell line, cell culture medium, retinoic acid, Tween 20, sodium oleate and other reagents were purchased from Sigma Aldrich. Antibodies raised against phospho-AKT pSer473, Phospho-IRS-1 pSer307, NF-kB2, and IL-1 DMEM/Glutamax medium; BCA Protein Assay Kit; RIPA buffer; phosphatase inhibitor cocktail; Trizol reagent; High Capacity Reverse Transcriptions Kit, TaqMan Advanced miRNA cDNA Synthesis Kit, miRNAs Advanced Taqman assays, lipofectamine, and RNAimax were purchased from Thermo Fisher Scientific, Inc. Antibodies raised against IRS-1, AKT, and -Actin anti-rabbit IgG HRP-linked antibodies were purchased from Cell Signaling Technology, Inc.
Heat-inactivated fetal bovine serum was purchased from PAN Biotech. I-Taq Universal SybrGreen Master Mix, protease inhibitor cocktail (BioRad), rno-pre-miR-193b and control pre-miR were purchased from Exiquon. PVDF membranes were obtained from Amersham. Immobilon Chemiluminescent Western HRP Substrate was obtained from Millipore. High Pure miRNA Isolation Kit was purchased from Roche Diagnostics, and Magnetic Luminex Screening Assay was purchased from R&D Systems. Caspase-1 antibody was obtained from Santa Cruz Biotechnology. ETS-1 antibody was obtained from Bethyl laboratories. NLRP3 antibody was obtained from Novus Biologicals. TXNIP antibody was obtained from Abgent.

Siddeek B., Li N., Mauduit C., Chehade H., Rigal E., Tolsa J.F., Armengaud J.B., Yzydorczyk C., Benahmed M., Vergely C, & Simeoni U. (2018). Transient postnatal over nutrition induces long-term alterations in cardiac NLRP3-inflammasome pathway. Nutrition, metabolism, and cardiovascular diseases : NMCD, 28(9).

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