A sample of 5 × 104 cultured erythroid cells was washed with PBS containing 0.1% BSA (PBSA) and suspended in 100 µL of PBSA containing anti-CD71 APC (dilution 1:50) (BD Pharmingen, San Jose, CA, USA) and anti-CD235a PE-Cy7 (dilution 1:50) (BD Pharmingen, San Jose, CA, USA). The cells were incubated in the dark with the antibodies for 20 min, washed with PBSA, and then analyzed on the Cytoflex LX Flow Cytometer (Beckmann Coulter, Indianapolis, IN, USA). The flow cytometry results were analyzed using FlowJo version 10.0.2 (Treestar, Ashland, OR, USA).
Anti cd71 apc
Manufactured by BD
Sourced in United States
Anti-CD71 APC is a fluorescently-labeled antibody that binds to the CD71 (transferrin receptor) protein expressed on the surface of various cell types. It can be used in flow cytometry applications to identify and analyze cells expressing CD71.
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Lab products found in correlation
2 protocols using anti cd71 apc
1
Erythroid Cell Immunophenotyping by Flow Cytometry
2
Quantifying Fetal Hemoglobin in Transduced HSPCs
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The frequency of F-cells in transduced HSPCs undergoing erythroid differentiation was quantified as previously described.8 (link) Briefly, cells were fixed in 0.05% glutaraldehyde for 10 min, permeabilized with 0.1% Triton X-100 (Life Technologies) for 5 min, and stained with an anti-HbF APC antibody (Invitrogen) for 30 min. Cells were subsequently washed, acquired on an Accuri C6 flow cytometer (BD Biosciences), and analyzed using FlowJo software (v.10.8.1, BD Biosciences).
To assess the impact of increased BACH2 expression on erythroid differentiation, transduced HSPCs undergoing erythroid differentiation were stained with a combination of anti-CD71 APC and CD235a PE (all from BD Biosciences) for 10 min. Stained cells were acquired on an Accuri C6 flow cytometer and analyzed using FlowJo software.
To assess the impact of increased BACH2 expression on erythroid differentiation, transduced HSPCs undergoing erythroid differentiation were stained with a combination of anti-CD71 APC and CD235a PE (all from BD Biosciences) for 10 min. Stained cells were acquired on an Accuri C6 flow cytometer and analyzed using FlowJo software.
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