The phalloidin, JC-1 and DCFH-DA probes were bought from Life Technology (St. Louis, MO, USA). PX-12, DAPI and isoprenaline hydrochloride (Iso) were purchased from Sigma-Aldrich (USA). Rhodamine-conjugated secondary antibodies were obtained from Jackson ImmunoResearch Laboratories (West Grove, PA, USA). STVNa was obtained from Chemical Development Laboratories of Key Biological Pharmaceutical Company (Dongguan, China). Trizol was obtained from Generay (Shanghai, China). Antibodies against Drp1, Trx1, Prdx2 and GAPDH were acquired from Cell Signaling Technology (Beverly, MA, USA). HDAC4 was obtained from Abcam (Cambridge, MA, UK). The Fis1 antibody and HRP-conjugated secondary antibodies were acquired from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Antibodies against drp1
Manufactured by Cell Signaling Technology
Sourced in United States
Antibodies against Drp1 are laboratory reagents used to detect and study the Dynamin-related protein 1 (Drp1), a key regulator of mitochondrial fission. These antibodies can be used in various experimental techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to visualize and quantify the expression levels of Drp1 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Hrp conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions)
Rhodamine conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Px 12, by Merck Group (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Hdac4, by Abcam (1 mentions)
Prdx2, by Cell Signaling Technology (1 mentions)
Isoprenaline hydrochloride iso, by Merck Group (1 mentions)
Trizol, by Generay (1 mentions)
2 protocols using antibodies against drp1
1
Mitochondrial Dynamics Regulation Protocol
2
AZT Impacts Mitochondrial Dynamics
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mitochondria were isolated from cells treated with 100 µM AZT or left untreated for 48 h. The effect of AZT on Drp1 and Opa1 expression was analyzed by western blotting using antibodies against Drp1(1: 1000), Mff (1: 1000), and phosphorylated (p-)Drp1 (1: 1000) (all from Cell Signaling Technology) and against Opa1 (1: 2000; BD Biosciences). Equal protein loading was confirmed using a rabbit antibody against voltage-dependent anion channel (1:1000; Sigma-Aldrich).
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