Western blotting was performed as described previously.47 (link) For animal samples, a total of 0.2 g peritoneum tissue for each animal was used to extract protein. Blots were incubated overnight at 4°C with polyclonal antibodies to periostin (Abcam), E-cadherin (BD Bioscience), α-SMA (Abcam), fibronectin (Dako), Snail (Abcam), GAPDH (Abcam), or β-actin (Sigma Chemical). The membranes were washed three times for 10 min in 1 × PBS with 0.1% Tween-20 and incubated in buffer A containing a 1:1,000 dilution of horseradish peroxidase-linked goat anti-rabbit or anti-mouse IgG (Santa Cruz Biotechnology). TINA image software (Raytest) was used to measure the band densities, and the changes in the optical densities of bands from the treated groups relative to untreated cells or tissues were used for analysis.
Horseradish peroxidase linked goat anti rabbit or anti mouse igg
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Horseradish peroxidase-linked goat anti-rabbit or anti-mouse IgG is a secondary antibody conjugate used in Western blotting, ELISA, and immunohistochemistry applications. It consists of goat-derived antibodies specific to rabbit or mouse immunoglobulin G (IgG), which are covalently linked to the enzyme horseradish peroxidase. This conjugate can be used to detect and visualize target proteins that have been recognized by primary antibodies raised in rabbit or mouse.
Automatically generated - may contain errors
Lab products found in correlation
Fibronectin, by Agilent Technologies (2 mentions)
Periostin, by Abcam (2 mentions)
Tina image software, by Elysia raytest (2 mentions)
β actin, by Merck Group (2 mentions)
α sma, by Abcam (1 mentions)
Snail, by Abcam (1 mentions)
Gapdh, by Abcam (1 mentions)
E cadherin, by BD (1 mentions)
Type 1 collagen, by Southern Biotech (1 mentions)
2 protocols using horseradish peroxidase linked goat anti rabbit or anti mouse igg
1
Protein Expression Analysis by Western Blotting
2
Quantitative Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Western blotting was performed as described previously46 (link). Blots were incubated overnight at 4 °C with polyclonal antibodies to periostin (Abcam, Cambridge, UK), fibronectin (Dako, Glostrup, Denmark), type I collagen (Southern Biotech, Birmingham, AL, USA), or β-actin (Sigma Chemical Co., Perth, Australia). The membranes were washed three times for 10 min in 1 × PBS with 0.1% Tween-20 and incubated in buffer A containing a 1:1000 dilution of horseradish peroxidase-linked goat anti-rabbit or anti-mouse IgG (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA). TINA image software (Raytest, Straubenhardt, Germany) was used to measure the band densities, and the changes in the optical densities of bands from the treated groups relative to control cells or tissues were used for analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!