Log In Sign up
The largest database of trusted experimental protocols

Ab15311 is a laboratory antibody product manufactured by Cell Signaling Technology. It is a primary antibody used in various immunoassay techniques.

Automatically generated - may contain errors

Lab products found in correlation

Ab110334, by Abcam (1 mentions) Protease and phosphatase inhibitor, by Merck Group (1 mentions) Ab115730, by Abcam (1 mentions) Hexokinase 1, by Cell Signaling Technology (1 mentions) Hexokinase 2, by Cell Signaling Technology (1 mentions) D9g3e, by Cell Signaling Technology (1 mentions) Odyssey imaging system, by LI COR (1 mentions) Glut3, by Abcam (1 mentions) Hif 1α, by Novus Biologicals (1 mentions) Glut1, by Merck Group (1 mentions)

2 protocols using ab15311

1

Phosphorylated PDH-E1α and Glucose Transporters

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in a low detergent buffer (1% Triton, 0.1% SDS) for one hour with protease and phosphatase inhibitors (Sigma-Aldrich). Nitrocellulose membranes were hybridized with anti-phospho S232-PDH-E1α (Millipore AP1063), total PDH-E1α antibodies (Abcam ab110334), Glut1 rabbit monoclonal (abcam, ab115730), Glut3 rabbit polyclonal (abcam, ab15311), actin (Cell Signaling, 4970S), cMyc (Cell Signaling, 179) or Hif1α (Cayman Chemicals 10006421).
Caro-Maldonado A., Wang R., Nichols A.G., Kuraoka M., Milasta S., Sun L.D., Gavin A.L., Abel E.D., Kelsoe G., Green D.R, & Rathmell J.C. (2014). Metabolic Reprogramming is Required for Antibody Production That is Suppressed in Anergic but Exaggerated in Chronically BAFF-Exposed B cells. Journal of immunology (Baltimore, Md. : 1950), 192(8), 3626-3636.
+ Open protocol
+ Expand
2

Investigating Cellular Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lysates from H460, A549, H1975, and HCC827 cells were prepared 24 h after treatment with DMSO, Milciclib (1 or 10 μM), THZ1 (10 μM), or LDC4297 (10 μM); 16 h after treatment with 1× PBS or Deferoxamine (100 μM); or 72 h after transfection with shRNA or an overexpression plasmid. Uncropped immunoblots are presented in Supplementary Fig. 29.
The following antibodies were used: HIF-1α (Novus Biologicals; NB100-105; 1:1000 dilution), GLUT1 (Millipore Sigma; 07-1401; 1:500 dilution), GLUT3 (Abcam; ab15311; 1:1000 dilution), Hexokinase 1 (Cell Signaling; C35C4; 1:1000 dilution), Hexokinase 2 (Cell Signaling; C64G5; 1:1000 dilution), CDK2 (Cell Signaling; 78B2; 1:1000 dilution), CDK4 (Cell Signaling; D9G3E; 1:1000 dilution), CDK7 (Cell Signaling; MO1; 1:1000 dilution), TRKA (Cell Signaling; 12G8; 1:1000 dilution), PIK3CA (Cell Signaling; C73F8; 1:1000 dilution), PTEN (Cell Signaling; 9559; 1:1000 dilution), Phospho-T170 CDK7 (Abcam; ab155976; 1:1000 dilution), Rpb1 NTD (Cell Signaling; D8L4Y; 1:1000 dilution), Phospho-S5 Rpb1 (Cell Signaling; D9N5I; 1:1000 dilution), PKCι (BD Biosciences; Clone 23; 1:1000 dilution), TXNIP (Cell Signaling; D5F3E; 1:1000 dilution), and Beta Actin (Imgenex; IMG-5142; 1:1000 dilution). Immunoblots were analyzed using an Odyssey Imaging System (LI-COR).
Ghezzi C., Wong A., Chen B.Y., Ribalet B., Damoiseaux R, & Clark P.M. (2019). A high-throughput screen identifies that CDK7 activates glucose consumption in lung cancer cells. Nature Communications, 10, 5444.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!