Human igg fc chimeric proteins

Manufactured by R&D Systems

Human IgG Fc chimeric proteins are recombinant fusion proteins that consist of the Fc region of human IgG antibodies linked to a specific protein or peptide. These proteins are commonly used in research applications to study protein-protein interactions, receptor-ligand binding, and other biological processes.

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Lab products found in correlation

P selectin, by R&D Systems (2 mentions) Anti human igg fc pe, by Thermo Fisher Scientific (2 mentions) Recombinant mouse il 15, by Thermo Fisher Scientific (1 mentions) Streptavidin fitc, by BioLegend (1 mentions) E selectin, by R&D Systems (1 mentions) Peanut agglutinin, by Vector Laboratories (1 mentions) Mal 2, by Vector Laboratories (1 mentions)

Spelling variants of this product

P- or E-selectin human IgG Fc chimeric proteins (2 citations) Recombinant rat JAG1/human Fc IgG chimeric protein (2 citations)

2 protocols using human igg fc chimeric proteins

Selectin Ligand Synthesis Assay

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To detect synthesis of P- and E-selectin ligands, WT and CD62L^-/- memory P14 CD8⁺ T cells from spleens were stimulated for 3 days with 250 ng/ml of recombinant mouse IL-15 (Peprotech). Recombinant E-selectin (5 μg/ml) and P-selectin (1.5 μg/ml) human IgG Fc chimeric proteins (R & D Systems) were incubated with cells for 30 minutes in 1% FBS/DPBS containing Ca²⁺ and Mg²⁺ (Gibco) at room temperature. Binding of selectins was detected using anti-human IgG-Fc PE (eBioscience). Cells were then stained with fluorescent antibodies as described in Flow Cytometry and Antibodies.

Osborn J.F., Hobbs S.J., Mooster J.L., Khan T.N., Kilgore A.M., Harbour J.C, & Nolz J.C. (2019). Central memory CD8+ T cells become CD69+ tissue-residents during viral skin infection independent of CD62L-mediated lymph node surveillance. PLoS Pathogens, 15(3), e1007633.

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Lectin and Selectin Binding Assay

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Fluorescein or biotin-conjugated Jacalin, Peanut Agglutinin (PNA), and MAL II (VectorLabs) were incubated with cells for 30 minutes in 1% FBS/PBS at room temperature. Binding of biotin-conjugated lectins was detected with FITC-Streptavidin (BioLegend). E-selectin (5 μg/ml) and P-selectin (1.5 μg/ml) human IgG Fc chimeric proteins (R & D Systems) were incubated with cells for 30 minutes in 1% FBS/DPBS containing Ca²⁺ and Mg²⁺ (Gibco) at room temperature. Binding of selectins was detected using anti-human IgG-Fc PE (eBioscience). Cells were then stained with fluorescent antibodies as described in Flow Cytometry and Antibodies.

Osborn J.F., Mooster J.L., Hobbs S.J., Munks M.W., Barry C., Harty J.T., Hill A.B, & Nolz J.C. (2017). Enzymatic Synthesis of Core 2 O-Glycans Governs the Tissue-Trafficking Potential of Memory CD8+ T Cells. Science immunology, 2(16), eaan6049.

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