To detect senescence-associated SA-β-galactosidase (SA-β-gal), HK-2 cells were stained using the SA-β-galactosidase (SA-β-gal) staining kit (Beyotime Biotechnology, Shanghai, China) according to the manufacturer’s instructions. Briefly, HK-2 cells with different treatments were fixed for 10 min, washed three times with PBS, and stained overnight at 37 °C. The next day, the cells were photographed and analyzed under a fluorescence microscope after washing with PBS three times.
Sa β galactosidase sa β gal staining kit
Manufactured by Beyotime
Sourced in China
The SA-β-galactosidase (SA-β-gal) staining kit is a laboratory tool used to detect and visualize senescence-associated β-galactosidase activity in cells. The kit provides the necessary reagents and protocols to perform this staining assay.
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Lab products found in correlation
Revertaid first strand cdna synthesis kit, by Thermo Fisher Scientific (2 mentions)
Immobilon p pvdf membrane, by Merck Group (1 mentions)
Goat anti rabbit igg ab6721, by Abcam (1 mentions)
Facs lysing solution, by BD (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Phenylmethylsulfonyl fluoride, by Merck Group (1 mentions)
2 protocols using sa β galactosidase sa β gal staining kit
1
Senescence Detection in HK-2 Cells
2
Senescence Marker Expression Analysis
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Anti-mouse CD45.1-FITC (clone A20) and anti-mouse CD45.2-PE (clone 104) antibodies were purchased from Biolegend (San Diego, CA, USA); anti-rabbit p16 (ab211542), anti-rabbit p21 (ab109199), anti- rabbit GADPH (ab181602) and goat anti-rabbit IgG (ab6721) antibodies were purchased from Abcam (Cambridge, MA, USA). FACSTM lysing solution was purchased from BD Biosciences (San Jose, CA, USA). An SA-β-galactosidase (SA-β-gal) staining kit was purchased from Beyotime Biotechnology (Nanjing, China). TRIzol was purchased from Life Technologies (Grand Island, NY, USA). A Revert Aid First Strand cDNA Synthesis Kit was purchased from Thermo Scientific (Waltham, MA, USA). RIPA buffer was purchased from BOSTER Biological Technology co.ltd (Wuhan, China). Phenylmethylsulfonyl fluoride was purchased from Sigma (St Louis, MO, USA). PVDF Immobilon-P membranes were purchased from Millipore (Billerica, MA, USA).
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