Blood samples were obtained from the inferior vena cava and were centrifugated at 3,000 rpm for 5 min to obtain plasma. Plasma levels of triglyceride (TG), total cholesterol (TC), alanine transaminase (ALT), aspartate transaminase (AST), amylase, creatinine, and urea nitrogen were analyzed by Triglyceride kit, Total cholesterol kit (Kehua, Shanghai, China), AST kit, ALT kit, amylase kit, creatinine kit, and urea nitrogen kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) respectively according to the manufacturer’s instruction.
Amylase kit
Manufactured by Nanjing Jiancheng
Sourced in China
The Amylase Kit is a laboratory product designed to measure the activity of the enzyme amylase in biological samples. Amylase is an enzyme that catalyzes the breakdown of starch into simpler sugars. The kit provides the necessary reagents and protocols to quantify the amylase levels.
Automatically generated - may contain errors
3 protocols using amylase kit
1
Plasma Metabolite Analysis Protocol
2
Comprehensive Analysis of Bioactive Compounds
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The kits for estimating SOD, amylase kit and coomassie brilliant blue kit were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Rutin (153-18-4) and gallic acid (149-91-7) standards were purchased from Chengdu DeSiTe Biological Technology Co., Ltd. (Chengdu, China). Glacial acetic acid standard (64-19-7) was purchased from Shanghai Acmec Biochemical Co., Ltd. (Shanghai, China). Twelve kinds of organic acid standards, namely, lactic acid (50-21-5), gallic acid (149-91-7), fumaric acid (110-17-8), sorbic acid (110-44-1), ascorbic acid (50-81-7), succinic acid (110-15-6), oxalic acid (144-62-7), shikimic acid (138-59-0), L-malic acid (97-67-6), citric acid (77-92-9), L-tartaric acid (87-69-4) and γ-aminobutyric acid (56-12-2) and foline-phenol were purchased from Beijing Solabao Science and Technology Co., Ltd. (Beijing, China). DPPH free radical was purchased from Tokyo Chemical Industry (Tokyo, Japan). All other chemicals and reagents used in this study were of analytical grade.
3
Serum Amylase Quantification Protocol
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Serum amylase levels were detected using the Amylase Kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacture’s instruction.
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